LOCUS       sequence-377071-        8677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Caspase conditional upon RNA transcript.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Jiang K, Koob J, Chen XD, Krajeski RN, Zhang Y, Volf V, Zhou W, 
            Sgrizzi SR, Villiger L, Gootenberg JS, Chen F, Abudayyeh OO
  TITLE     Programmable eukaryotic protein synthesis with RNA sensors by 
            harnessing ADAR.
  JOURNAL   Nat Biotechnol. 2022 Oct 27. pii: 10.1038/s41587-022-01534-5. doi: 
            10.1038/s41587-022-01534-5.
  PUBMED    36302988
REFERENCE   2  (bases 1 to 8677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Oct 27. pii: 10.1038/s41587-022-01534-5. doi: 
            10.1038/s41587-022-01534-5."
COMMENT     Sequence Label: pDY1462_SERPINA1_CCA32_Caspase
FEATURES             Location/Qualifiers
     source          1..8677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    109..130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(145..150,151..168,169..175)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    183..199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     188..210
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     207..223
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     207..223
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    237..285
                     /note="synthetic polyadenylation signal"
     CDS             complement(286..1947)
                     /codon_start=1
                     /product="secreted Cypridina luciferase"
                     /label=CLuc
                     /note="contains 30 codon substitutions for efficient 
                     translation in mammalian cells"
                     /translation="MKTLILAVALVYCATVHCQDCPYEPDPPNTVPTSCEAKEGECIDS
                     SCGTCTRDILSDGLCENKPGKTCCRMCQYVIECRVEAAGWFRTFYGKRFQFQEPGTYVL
                     GQGTKGGDWKVSITLENLDGTKGAVLTKTRLEVAGDIIDIAQATENPITVNGGADPIIA
                     NPYTIGEVTIAVVEMPGFNITVIEFFKLIVIDILGGRSVRIAPDTANKGMISGLCGDLK
                     MMEDTDFTSDPEQLAIQPKINQEFDGCPLYGNPDDVAYCKGLLEPYKDSCRNPINFYYY
                     TISCAFARCMGGDERASHVLLDYRETCAAPETRGTCVLSGHTFYDTFDKARYQFQGPCK
                     EILMAADCFWNTWDVKVSHRNVDSYTEVEKVRIRKQSTVVELIVDGKQILVGGEAVSVP
                     YSSQNTSIYWQDGDILTTAILPEALVVKFNFKQLLVVHIRDPFDGKTCGICGNYNQDFS
                     DDSFDAEGACDLTPNPPGCTEEQKPEAERLCNSLFAGQSDLDQKCNVCHKPDRVERCMY
                     EYCLRGQQGFCDHAWEFKKECYIKHGDTLEVPDECK"
     primer_bind     complement(1972..1991)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1973..1991)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2036..2239)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2065..2085)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2240..2619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2733..3911
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2964..3902
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3859..3879
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3924..3933
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3930..4637
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4075..4093)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4343..4362
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4908..4964
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4980..5300
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             complement(5652..5663)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             6177..6203
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6213..6261
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(6284..6301)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6284..6300)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6293..6315)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6509..6528)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6628..6650
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6688..6706)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6774..6878
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6879..6947,6948..7739)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSGGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7097..7116)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7910..8498
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8399..8418
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8652..8669
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
       61 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      121 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      181 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccacaca
      241 caaaaaacca acacacagat gtaatgaaaa taaagatatt ttattttatt tgcattcatc
      301 tggtacttct agggtgtctc catgctttat gtagcattct ttcttgaact cccatgcgtg
      361 gtcacagaaa ccctgttgtc ccctcaggca atactcgtac atgcatcgtt cgacacggtc
      421 aggcttgtgg cacacgttac atttctgatc aagatcactt tgaccggcga agagactatt
      481 gcagagtcgt tcagcttcag gtttctgttc ttcggtgcat cccggtgggt tgggggtcag
      541 atcacaggct ccttcagcat caaaagaatc atcactgaaa tcctggttgt agttaccgca
      601 aataccgcaa gtcttaccat cgaatggatc tctaatatgt acgacgagca gttgcttgaa
      661 gttgaacttg accaccagag cttcaggtag gatggctgta gtcagtatgt caccatcttg
      721 ccagtagatg gaagtgttct gagagctgta cgggacggac acggcttctc ctccaaccag
      781 aatctgtttt ccatcaacaa tgagttctac tacagtcgat tgtttcctga ttcgtacttt
      841 ctctacttca gtgtaagagt caacattcct gtgtgaaacc ttcacatccc aagtgttcca
      901 gaaacagtcg gcggccataa gaatctcctt gcagggaccc tggaattggt atcttgcttt
      961 gtcaaatgta tcgtagaaag tatgtccaga caaaacgcag gttcctctag tttcgggagc
     1021 agcgcacgtc tccctgtagt caagcagcac gtgtgaggct cgctcgtctc cacccataca
     1081 gcgggcgaag gcgcaggaga tggtgtagta gtagaagttg atggggttgc ggcagctgtc
     1141 cttgtacggc tccagaagac ctttgcagta tgcaacgtca tcaggatttc catagagtgg
     1201 acaaccgtca aactcctggt tgatcttagg ctgaatagcg agttgttctg gatctgaagt
     1261 gaagtctgta tcttccatca ttttaagatc tccacagagg ccagagatca ttcctttgtt
     1321 tgctgtgtct ggggcgattc ttacagatct tcctccgagg atgtcgatca cgatcagttt
     1381 gaagaactca atgacggtga tgttgaagcc tggcatctca acaacagcga tggtgacctc
     1441 gccgatggtg tacgggttgg cgatgatagg gtcagctcca ccgtttacag tgatgggatt
     1501 ctcagtagct tgagcgatgt caatgatgtc tccagccact tccagtcttg tcttggtcag
     1561 cacagccccc ttggttccat ccaggttctc cagggtgatg gacaccttcc agtcgccgcc
     1621 cttggttcct tgacccaaca cgtatgtacc aggttcctgg aactggaatc tctttccata
     1681 gaatgttcta aaccatcctg cggcctctac tctgcattca attacatact gacacattcg
     1741 acaacatgtt tttcctggtt tattttcaca cagtccatct gatagtatgt ctctcgtgca
     1801 ggtgccacag ctgctatcaa tacattctcc ttctttagct tcacaggaag ttggaactgt
     1861 gtttggtgga tcaggttcgt aaggacagtc ctggcaatga acagtggcgc agtagactaa
     1921 tgcaacggca agaattaagg tcttcatggt ggcggtacca agcttgcgtc tccctatagt
     1981 gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc cagagagctc
     2041 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
     2101 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
     2161 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
     2221 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
     2281 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     2341 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
     2401 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
     2461 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
     2521 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
     2581 taattgatta ctattaataa ctagtcaata atcaatgtca acgcgtatat ctggcccgta
     2641 catcgaagct gatgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat
     2701 ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc
     2761 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
     2821 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     2881 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     2941 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
     3001 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
     3061 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
     3121 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa
     3181 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     3241 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     3301 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     3361 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     3421 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     3481 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     3541 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     3601 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     3661 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     3721 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga
     3781 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     3841 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc
     3901 aggtgtcgtg acgtacggga tccgccacca tggtgagcaa gggcgaggag gataacatgg
     3961 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     4021 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     4081 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     4141 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     4201 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     4261 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     4321 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     4381 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     4441 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     4501 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     4561 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     4621 tggacgagct gtacaagtct agagagaggt cagccccatt gctgaagacc acgggtggag
     4681 gatcacccca cccggatgcc cagttgaccc aggacgctct taagggtgga ggaacacccc
     4741 accctcatag gttccagtac tggacagttt aggtaaatgt aagctggcag accttcacag
     4801 aagcaccatc agggcttctg tcattttcca ggaacttggt gaggatagtg cgtggagcat
     4861 cagcccacgc aggtgagttc attttccagg tgctgtgggg aagcggagct actaacttca
     4921 gcctgctgaa gcaggctggc gacgtggagg agaaccctgg acctgtaggt agggtcgagg
     4981 gagtgcaggt ggagactatc tccccaggag acgggcgcac cttccccaag cgcggccaga
     5041 cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaagttgat tcctcccggg
     5101 acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga ggctgggaag
     5161 aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct ccagattatg
     5221 cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc gtcttcgatg
     5281 tggagcttct aaaactggaa tctggcggtg gatccggagt cgacggattt ggtgatgtcg
     5341 gtgctcttga gagtttgagg ggaaatgcag atttggctta catcctgagc atggagccct
     5401 gtggccactg cctcattatc aacaatgtga acttctgccg tgagtccggg ctccgcaccc
     5461 gcactggctc caacatcgac tgtgagaagt tgcggcgtcg cttctcctcg ctgcatttca
     5521 tggtggaggt gaagggcgac ctgactgcca agaaaatggt gctggctttg ctggagctgg
     5581 cgcggcagga ccacggtgct ctggactgct gcgtggtggt cattctctct cacggctgtc
     5641 aggccagcca cctgcagttc ccaggggctg tctacggcac agatggatgc cctgtgtcgg
     5701 tcgagaagat tgtgaacatc ttcaatggga ccagctgccc cagcctggga gggaagccca
     5761 agctcttttt catccaggcc tgtggtgggg agcagaaaga ccatgggttt gaggtggcct
     5821 ccacttcccc tgaagacgag tcccctggca gtaaccccga gccagatgcc accccgttcc
     5881 aggaaggttt gaggaccttc gaccagctgg acgccatatc tagtttgccc acacccagtg
     5941 acatctttgt gtcctactct actttcccag gttttgtttc ctggagggac cccaagagtg
     6001 gctcctggta cgttgaaacc ctggacgaca tctttgagca gtgggctcac tctgaagacc
     6061 tgcagtccct cctgcttagg gtcgctaatg ctgtttcggt gaaagggatt tataaacaga
     6121 tgcctggttg ctttaatttc ctccggaaaa aacttttctt taaaacatca gtcgactatc
     6181 cgtacgacgt accagactac gcactcgact aaaataaaat atctttattt tcattacatc
     6241 tgtgtgttgg ttttttgtgt gcccgggtac cgagctcgaa ttcactggcc gtcgttttac
     6301 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     6361 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     6421 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     6481 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     6541 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     6601 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     6661 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     6721 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     6781 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6841 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6901 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6961 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7021 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7081 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7141 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7201 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7261 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7321 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7381 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7441 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7501 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7561 ttattgctga taaatctgga gccggtgagc gtgggtctgg cggtatcatt gcagcactgg
     7621 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7681 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7741 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7801 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7861 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7921 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7981 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8041 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8101 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8161 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8221 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8281 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8341 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8401 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8461 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8521 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8581 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8641 cgaccgagcg cagcgagtca gtgagcgagg aagcgga
//
