LOCUS       sequence-377074-        9728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9728)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 9728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: TERT
FEATURES             Location/Qualifiers
     source          1..9728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1196..1215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1293..1317
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(4336..4347)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(4685..4709)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4834..4875
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5330..5347)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5336..5560
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5693..5712)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5844..5891
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5899..6921
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5899..6921
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7051..7172
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7088..7107)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7142..7161
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7221..7237)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7221..7237)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7234..7256)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7245..7261
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7269..7275,7276..7293,7294..7299))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7314..7335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7452..7469)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7623..8211)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7703..7722)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8382..9173,9174..9242))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9005..9024
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9243..9347)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9422..9441)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac
     1261 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggctgcc
     1321 accatgccgc gcgctccccg ctgccgagcc gtgcgctccc tgctgcgcag ccactaccgc
     1381 gaggtgctgc cgctggccac gttcgtgcgg cgcctggggc cccagggctg gcggctggtg
     1441 cagcgcgggg acccggcggc tttccgcgcg ctggtggccc agtgcctggt gtgcgtgccc
     1501 tgggacgcac ggccgccccc cgccgccccc tccttccgcc aggtgtcctg cctgaaggag
     1561 ctggtggccc gagtgctgca gaggctgtgc gagcgcggcg cgaagaacgt gctggccttc
     1621 ggcttcgcgc tgctggacgg ggcccgcggg ggcccccccg aggccttcac caccagcgtg
     1681 cgcagctacc tgcccaacac ggtgaccgac gcactgcggg ggagcggggc gtgggggctg
     1741 ctgctgcgcc gcgtgggcga cgacgtgctg gttcacctgc tggcacgctg cgcgctcttt
     1801 gtgctggtgg ctcccagctg cgcctaccag gtgtgcgggc cgccgctgta ccagctcggc
     1861 gctgccactc aggcccggcc cccgccacac gctagtggac cccgaaggcg tctgggatgc
     1921 gaacgggcct ggaaccatag cgtcagggag gccggggtcc ccctgggcct gccagccccg
     1981 ggtgcgagga ggcgcggggg cagtgccagc cgaagtctgc cgttgcccaa gaggcccagg
     2041 cgtggcgctg cccctgagcc ggagcggacg cccgttgggc aggggtcctg ggcccacccg
     2101 ggcaggacgc gtggaccgag tgaccgtggt ttctgtgtgg tgtcacctgc cagacccgcc
     2161 gaagaagcca cctctttgga gggtgcgctc tctggcacgc gccactccca cccatccgtg
     2221 ggccgccagc accacgcggg ccccccatcc acatcgcggc caccacgtcc ctgggacacg
     2281 ccttgtcccc cggtgtacgc cgagaccaag cacttcctct actcctcagg cgacaaggag
     2341 cagctgcggc cctccttcct actcagctct ctgaggccca gcctgactgg cgctcggagg
     2401 ctcgtggaga ccatctttct gggttccagg ccctggatgc cagggactcc ccgcaggttg
     2461 ccccgcctgc cccagcgcta ctggcaaatg cggcccctgt ttctggagct gcttgggaac
     2521 cacgcgcagt gcccctacgg ggtgctcctc aagacgcact gcccgctgcg agctgcggtc
     2581 accccagcag ccggtgtctg tgcccgggag aagccccagg gctctgtggc ggcccccgag
     2641 gaggaggaca cagacccccg tcgcctggtg cagctgctcc gccagcacag cagcccctgg
     2701 caggtgtacg gcttcgtgcg ggcctgcctg cgccggctgg tgcccccagg cctctggggc
     2761 tccaggcaca acgaacgccg cttcctcagg aacaccaaga agttcatctc cctggggaag
     2821 catgccaagc tctcgctgca ggagctgacg tggaagatga gcgtgcggga ctgcgcttgg
     2881 ctgcgcagga gcccaggggt tggctgtgtt ccggccgcag agcaccgtct gcgtgaggag
     2941 atcctggcca agttcctgca ctggctgatg agtgtgtacg tcgtcgagct gctcaggtct
     3001 ttcttttatg tcacggagac cacgtttcaa aagaacaggc tctttttcta ccggaagagt
     3061 gtctggagca agttgcaaag cattggaatc agacagcact tgaagagggt gcagctgcgg
     3121 gagctgtcgg aagcagaggt caggcagcat cgggaagcca ggcccgccct gctgacgtcc
     3181 agactccgct tcatccccaa gcctgacggg ctgcggccga ttgtgaacat ggactacgtc
     3241 gtgggagcca gaacgttccg cagagaaaag agggccgagc gtctcacctc gagggtgaag
     3301 gcactgttca gcgtgctcaa ctacgagcgg gcgcggcgcc ccggcctcct gggcgcctct
     3361 gtgctgggcc tggacgatat ccacagggcc tggcgcacct tcgtgctgcg tgtgcgggcc
     3421 caggacccgc cgcctgagct gtactttgtc aaggacaggc tcacggaggt catcgccagc
     3481 atcatcaaac cccagaacac gtactgcgtg cgtcggtatg ccgtggtcca gaaggccgcc
     3541 catgggcacg tccgcaaggc cttcaagagc cacgtctcta ccttgacaga cctccagccg
     3601 tacatgcgac agttcgtggc tcacctgcag gagaccagcc cgctgaggga tgccgtcgtc
     3661 atcgagcaga gctcctccct gaatgaggcc agcagtggcc tcttcgacgt cttcctacgc
     3721 ttcatgtgcc accacgccgt gcgcatcagg ggcaagtcct acgtccagtg ccaggggatc
     3781 ccgcagggct ccatcctctc cacgctgctc tgcagcctgt gctacggcga catggagaac
     3841 aagctgtttg cggggattcg gcgggacggg ctgctcctgc gtttggtgga tgatttcttg
     3901 ttggtgacac ctcacctcac ccacgcgaaa accttcctca ggaccctggt ccgaggtgtc
     3961 cctgagtatg gctgcgtggt gaacttgcgg aagacagtgg tgaacttccc tgtagaagac
     4021 gaggccctgg gtggcacggc ttttgttcag atgccggccc acggcctatt cccctggtgc
     4081 ggcctgctgc tggatacccg gaccctggag gtgcagagcg actactccag ctatgcccgg
     4141 acctccatca gagccagtct caccttcaac cgcggcttca aggctgggag gaacatgcgt
     4201 cgcaaactct ttggggtctt gcggctgaag tgtcacagcc tgtttctgga tttgcaggtg
     4261 aacagcctcc agacggtgtg caccaacatc tacaagatcc tcctgctgca ggcgtacagg
     4321 tttcacgcat gtgtgctgca gctcccattt catcagcaag tttggaagaa ccccacattt
     4381 ttcctgcgcg tcatctctga cacggcctcc ctctgctact ccatcctgaa agccaagaac
     4441 gcagggatgt cgctgggggc caagggcgcc gccggccctc tgccctccga ggccgtgcag
     4501 tggctgtgcc accaagcatt cctgctcaag ctgactcgac accgtgtcac ctacgtgcca
     4561 ctcctggggt cactcaggac agcccagacg cagctgagtc ggaagctccc ggggacgacg
     4621 ctgactgccc tggaggccgc agccaacccg gcactgccct cagacttcaa gaccatcctg
     4681 gacgacccag ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgagt
     4741 ctagagggcc cgcggttcga aaccagcgga ggcggcggaa gcgggggagg aggcagcggc
     4801 ggaggcggca gcgggggcgg cggaagcgct accggtaagc ctatccctaa ccctctcctc
     4861 ggtctcgatt ctacgcgtac cggtatggct tctaacttta ctcagttcgt tctcgtcgac
     4921 aatggcggaa ctggcgacgt gactgtcgcc ccaagcaact tcgctaacgg ggtcgctgaa
     4981 tggatcagct ctaactcgcg atcacaggct tacaaagtaa cctgtagcgt tcgtcagagc
     5041 tctgcgcaga atcgcaaata caccatcaaa gtcgaggtgc ctaaagtggc aacccagact
     5101 gttggtggtg aagagcttcc tgtagccgga tggagatctt acttaaatat ggaactaacc
     5161 attccaattt tcgccacgaa ttccgactgc gagcttattg ttaaggcaat gcaaggtctc
     5221 ctaaaagatg gaaacccgat tccctcggcc atcgcggcca actccggcat ctacggaggt
     5281 ggaggtggat ctcgtacggg ttagtaatga gtttaaaccc gctgatcagc ctcgactgtg
     5341 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     5401 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     5461 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     5521 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     5581 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5641 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5701 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5761 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5821 tagggtgatg gttcacgtac ctagaagttc ctattccgaa gttcctattc tctagaaagt
     5881 ataggaactt ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     5941 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     6001 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     6061 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     6121 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     6181 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     6241 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     6301 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     6361 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     6421 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     6481 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     6541 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     6601 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     6661 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     6721 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     6781 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     6841 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     6901 cgtccgaggg caaaggaata gcacgtacta cgagatttcg attccaccgc cgccttctat
     6961 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     7021 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     7081 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7141 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     7201 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7261 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7321 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7381 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7441 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7501 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7561 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7621 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7681 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7741 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7801 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7861 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7921 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7981 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8041 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8101 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8161 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8221 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8281 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8341 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8401 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8461 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8521 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8581 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8641 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8701 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8761 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8821 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8881 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8941 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9001 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9061 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9121 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9181 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9241 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9301 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9361 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     9421 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     9481 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     9541 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     9601 gatatacgcg ttgacattga ttattgacta ggcttttgca aaaagctttg caaagatgga
     9661 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag
     9721 tgggaatt
//
