LOCUS       sequence-377089-         417 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the de novo luciferase, LuxSit-i.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Yeh AH, Norn C, Kipnis Y, Tischer D, Pellock SJ, Evans D, Ma P, Lee 
            GR, Zhang JZ, Anishchenko I, Coventry B, Cao L, Dauparas J, Halabiya
            S, DeWitt M, Carter L, Houk KN, Baker D
  TITLE     De novo design of luciferases using deep learning.
  JOURNAL   Nature. 2023 Feb;614(7949):774-780. doi: 10.1038/s41586-023-05696-3.
            Epub 2023 Feb 22.
  PUBMED    36813896
REFERENCE   2  (bases 1 to 417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-05696-3"; journalName: "Nature"; date: 
            "2023-02"; volume: "614"; issue: "7949"; pages: "774-780"
COMMENT     Sequence Label: pET29_LuxSit_i
FEATURES             Location/Qualifiers
     source          1..417
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..27
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..60
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggtagcc atcaccacca tcaccatggt agcggtagcg agaacttgta cttccaagga
       61 atgagcgaag aacagattcg tcagtttctg cgtcgttttt atgaagcgct ggatagcggc
      121 gatgcggata ccgctgcgag cctgtttcat ccgggcgtga caattcatct gtgggatggc
      181 gttaccttta ccagccgtga agaatttcgt gaatggtttg aacgtctgtt tagcaccagt
      241 aaagatgcgc agcgtgaaat taagagcctg gaagtacgtg gcgataccgt ggaagtgcat
      301 gtgcagttgc acgcgaccca taatggccag aaacataccg tagatttgac ccatcattgg
      361 cattttcgtg gcaatcgtgt gaccgaagtt cgtgtgcata tcaatccgac cggctaa
//
