LOCUS       sequence-377098-       12131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SpCas9 expression in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12131)
  AUTHORS   Lawrenson T, Youles M, Chhetry M, Clarke M, Harwood W, Hundleby P
  TITLE     Efficient Targeted Mutagenesis in Brassica Crops Using CRISPR/Cas 
            Systems.
  JOURNAL   Methods Mol Biol. 2023;2653:253-271. doi: 
            10.1007/978-1-0716-3131-7_16.
  PUBMED    36995631
REFERENCE   2  (bases 1 to 12131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2023"; volume: "2653:253-271. doi"; pages: " 
            10.1007/978-1-0716-3131-7_16"
COMMENT     Sequence Label: pICSL11197
FEATURES             Location/Qualifiers
     source          1..12131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    81..105
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      197..828
                     /label=oriV
                     /note="incP origin of replication"
     promoter        915..1019
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1020..1088,1089..1880)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1238..1257)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2051..2639
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2540..2559
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2975..4123
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4279..4303
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5692..5712
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11445..11465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 acagagtggg gcccactgca tccaccccag tacattaaaa acgtccgcaa tgtgttatta
       61 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
      121 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtcaga
      181 tcaggatctc ctttgcgacg ctcaccgggc tggttgccct cgccgctggg ctggcggccg
      241 tctatggccc tgcaaacgcg ccagaaacgc cgtcgaagcc gtgtgcgaga caccgcggcc
      301 gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga ggggcggacg
      361 ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg caggctcgat
      421 ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg
      481 cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg tattgacact
      541 tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag gggcagagtg
      601 ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc agaaaatcca
      661 gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt taacctgctt
      721 ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc gcgtgaccgc
      781 gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa cgcgggcctc
      841 ccatcccccc aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa aatggcagcg
      901 ctggccaatt cgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      961 atccgctcat gagacaataa ccttgataaa tgcttcaata atattgaaaa aggaagagta
     1021 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     1081 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     1141 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     1201 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     1261 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     1321 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     1381 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     1441 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     1501 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1561 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1621 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1681 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggttctc
     1741 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1801 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1861 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1921 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1981 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     2041 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2101 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2161 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2221 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2281 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2341 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2401 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2461 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2521 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2581 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2641 acgccagcaa cgcggccttt ttacggttcc tggcagatcc tagatgtggc gcaacgatgc
     2701 cggcgacaag caggagcgca ccgacttctt ccgcatcaag tgttttggct ctcaggccga
     2761 ggcccacggc aagtatttgg gcaaggggtc gctggtattc gtgcagggca agattcggaa
     2821 taccaagtac gagaaggacg gccagacggt ctacgggacc gacttcattg ccgataaggt
     2881 ggattatctg gacaccaagg caccaggcgg gtcaaatcag gaataagggc acattgcccc
     2941 ggcgtgagtc ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg accggaaggc
     3001 atacaggcaa gaactgatcg acgcggggtt ttccgccgag gatgccgaaa ccatcgcaag
     3061 ccgcaccgtc atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga tggtccagca
     3121 agctacggcc aagatcgagc gcgacagcgt gcaactggct ccccctgccc tgcccgcgcc
     3181 atcggccgcc gtggagcgtt cgcgtcgtct tgaacaggag gcggcaggtt tggcgaagtc
     3241 gatgaccatc gacacgcgag gaactatgac gaccaagaag cgaaaaaccg ccggcgagga
     3301 cctggcaaaa caggtcagcg aggccaagca ggccgcgttg ctgaaacaca cgaagcagca
     3361 gatcaaggaa atgcagcttt ccttgttcga tattgcgccg tggccggaca cgatgcgagc
     3421 gatgccaaac gacacggccc gctctgccct gttcaccacg cgcaacaaga aaatcccgcg
     3481 cgaggcgctg caaaacaagg tcattttcca cgtcaacaag gacgtgaaga tcacctacac
     3541 cggcgtcgag ctgcgggccg acgatgacga actggtgtgg cagcaggtgt tggagtacgc
     3601 gaagcgcacc cctatcggcg agccgatcac cttcacgttc tacgagcttt gccaggacct
     3661 gggctggtcg atcaatggcc ggtattacac gaaggccgag gaatgcctgt cgcgcctaca
     3721 ggcgacggcg atgggcttca cgtccgaccg cgttgggcac ctggaatcgg tgtcgctgct
     3781 gcaccgcttc cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg tcctgatcga
     3841 cgaggaaatc gtcgtgctgt ttgctggcga ccactacacg aaattcatat gggagaagta
     3901 ccgcaagctg tcgccgacgg cccgacggat gttcgactat ttcagctcgc accgggagcc
     3961 gtacccgctc aagctggaaa ccttccgcct catgtgcgga tcggattcca cccgcgtgaa
     4021 gaagtggcgc gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg gcctggtgga
     4081 acacgcctgg gtcaatgatg acctggtgca ttgcaaacgc tagggccttg tggggtcagt
     4141 tccggctggg ggttcagcag ccagcgcctg atctggggaa ccctgtggtt ggcatgcaca
     4201 tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat tattctaata
     4261 aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa
     4321 ccacttcgtg cagaagacaa tagtggaggt cgagctgcag gtcaacggat caggatattc
     4381 ttgtttaaga tgttgaactc tatggaggtt tgtatgaact gatgatctag gaccggataa
     4441 gttcccttct tcatagcgaa cttattcaaa gaatgttttg tgtatcattc ttgttacatt
     4501 gttattaatg aaaaaatatt attggtcatt ggactgaaca cgagtgttaa atatggacca
     4561 ggccccaaat aagatccatt gatatatgaa ttaaataaca agaataaatc gagtcaccaa
     4621 accacttgcc ttttttaacg agacttgttc accaacttga tacaaaagtc attatcctat
     4681 gcaaatcaat aatcatacaa aaatatccaa taacactaaa aaattaaaag aaatggataa
     4741 tttcacaata tgttatacga taaagaagtt acttttccaa gaaattcact gattttataa
     4801 gcccacttgc attagataaa tggcaaaaaa aaacaaaaag gaaaagaaat aaagcacgaa
     4861 gaattctaga aaatacgaaa tacgcttcaa tgcagtggga cccacggttc aattattgcc
     4921 aattttcagc tccaccgtat atttaaaaaa taaaacgata atgctaaaaa aatataaatc
     4981 gtaacgatcg ttaaatctca acggctggat cttatgacga ccgttagaaa ttgtggttgt
     5041 cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg agtcgtgttt
     5101 atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta gccaaaaaca
     5161 actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta ttgcttcacc
     5221 gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt ctataaaaca
     5281 atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa tcttaaaaac
     5341 tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta ttctctcaaa
     5401 atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt ttagattctg
     5461 ttaatcttag atcgaacacg attttctggg tttgatcgtt agatatcatc ttaattctcg
     5521 attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg tcgaataatt
     5581 actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg tgcgatcgaa
     5641 tttgtcgatt aatctgagtt tttctgatta acaggaatgg cttctagccc accgaagaag
     5701 aagcggaagg tcagctggaa aatggacaag aagtacagca ttggacttga tattggtacg
     5761 aactcagttg ggtgggccgt tatcaccgat gaatacaagg taccttcgaa gaaatttaaa
     5821 gtgctgggca acacagatag gcacagcatt aagaagaact tgatcggagc tctgctcttt
     5881 gactctggag aaaccgcgga ggcgacaagg cttaaacgta ctgcgaggag aaggtacact
     5941 cgcaggaaga acagaatctg ttatctccaa gagatcttta gcaacgagat ggcgaaggta
     6001 aggattttta tgatatagta tgcttatgta ttttgtactg aaagcatatc ctgcttcatt
     6061 gggatattac tgaaagcatt taactacatg taaactcact tgatgatcaa taaacttgat
     6121 tttgcaggtt gacgactcgt tcttccatcg cctcgaggaa tccttcctgg tagaggaaga
     6181 taagaaacac gagcgtcacc ccatctttgg gaatattgtt gacgaagtag cctatcatga
     6241 aaagtatccg actatatacc accttcgcaa gaagctggtg gactcaaccg ataaggcaga
     6301 ccttcggctc atatacctgg ctctcgcgca catgataaag tttcgtggcc atttcttgat
     6361 cgaaggggac ctcaacccgg ataactccga tgtggataaa ctgttcattc agctcgtcca
     6421 aacctacaat cagctgttcg aggagaaccc catcaatgca tcaggtaaca ttccttagtt
     6481 acctttcttt tctttttcca tcataagttt atagattgta catgctttga gatttttctt
     6541 tgcaaacaat ctcaggtgtc gacgccaagg caatactgtc tgccagactt tcgaagtcca
     6601 gacggcttga gaatctgatc gctcaattgc caggcgagaa gaagaacggc ttgttcggga
     6661 atctgattgc actgtctctg ggcctcaccc ctaacttcaa aagcaacttt gacctcgccg
     6721 aggacgcgaa gctgcagctg tcaaaggata catacgatga tgatctggac aatctgctcg
     6781 cccaaatagg tgctcttgaa attggaactc ttcttttgtt gtctaaacct atcaatttct
     6841 ttgcggaaat ttatttgaag ctgtagagtt aaaattgagt cttttaaact tttgtaggtg
     6901 atcagtatgc cgacctgttc ttggctgcca agaatctgtc agacgctatc ttgctcagtg
     6961 acattctgcg ggtcaacacg gagataacca aagcgccact tagcgcctcc atgatcaaga
     7021 ggtacgacga gcatcaccag gatctgaccc ttctgaaggc tttggttcgc cagcaactcc
     7081 ccgagaagta caaggagatt ttctttgacc aatcgaagaa tggctacgca gggtacattg
     7141 atggaggtaa gttgttactt atgattgttt tcctctctgc tacatgtatt ttgttgttca
     7201 tttctgtaag atataagaat tgagttttcc tctgatgata ttattaggtg caagtcagga
     7261 ggaattctac aaattcatca agcctattct ggaaaagatg gacggtacag aggagctgct
     7321 cgttaaattg aaccgcgaag atttgcttcg gaagcagcgt accttcgaca atggcagcat
     7381 accgcaccag atccacctcg gtgagctgca tgctatcttg aggaggcaag aggacttcta
     7441 tccgttcctg aaagacaaca gagagaagat tgaaaagatc ctcacgttcc gcattcccta
     7501 ctatgtaggt tagtatcata tgaagaaata cctagtttca gttgatgaat gctattttct
     7561 gacctcagtt gttctctttt gagaattatt tcttttctaa tttgcctgat ttttctatta
     7621 attcattagg tccactcgca cgcgggaact cgcggtttgc gtggatgaca cgcaaatccg
     7681 aggagactat cacgccttgg aacttcgaag aggtcgtgga caagggtgcg agtgcacagt
     7741 ccttcatcga aaggatgacc aacttcgata agaatctccc aaatgagaaa gtcctgccca
     7801 agcatagtct cctgtacgaa tacttcacgg tctacaacga gctgacgaag gtgaaatatg
     7861 tgacggaggg gatgcgcaaa ccggccttcc tgtcaggtaa atcctggtcc acacttttac
     7921 gataaaaaca caagatttta aactatgaac tgatcaataa tcattcctaa aagaccacac
     7981 ttttgttttg tttctaaagt aatttttact gttataacag gtgagcagaa gaaggccatt
     8041 gtcgatctct tgttcaaaac caatcggaag gtcactgtga aacagcttaa agaggactac
     8101 tttaagaaga tcgaatgctt tgattctgtg gaaatcagcg gcgttgagga taggttcaat
     8161 gcctctcttg gcacatacca tgacctgttg aaaatcatca aggacaagga cttccttgac
     8221 aacgaggaga acgaggacat cctcgaggac atcgtgctga ctctcacgct gtttgaggac
     8281 agagaaatga tcgaggagcg ccttaagact tatgcgcatc tgttcgatga caaggtcatg
     8341 aagcagttga agaggaggag atatacaggt aagaggtcaa aaggtttccg caatgatccc
     8401 tctttttttg tttctctagt ttcaagaatt tgggtatatg actaacttct gagtgttcct
     8461 tgatgcatat ttgtgatgag acaaatgttt gttctatgtt ttaggttggg gaaggctctc
     8521 caggaagctc atcaacggca tccgcgacaa gcaatccggc aagactatac tggactttct
     8581 caaatccgac ggttttgcga atcggaactt catgcagctt attcacgatg actcactgac
     8641 cttcaaagaa gatatccaga aggcccaagt gtcaggtcag ggcgatagcc ttcacgaaca
     8701 catagccaac ctggctggat cgccagctat aaagaagggc atactgcaga cagtgaaggt
     8761 tgtggatgag ctggtgaagg taagttctgc atttggttat gctccttgca ttttaggtgt
     8821 tcgtcgcact tccatttcca tgaatagcta agattttttt tctctgcatt cattcttctt
     8881 gcctcagttc taactgtttg tggtattttt gttttaatta ttgctacagg tcatgggccg
     8941 ccataagccg gagaacatcg tcatcgagat ggcgagggaa aaccagacga ctcagaaagg
     9001 gcagaagaac tcacgggagc gcatgaagcg gatagaggaa ggcatcaagg agcttgggag
     9061 tcagattctg aaagagcacc cagtcgaaaa tactcaactc cagaacgaga agctgtacct
     9121 ctattacctc cagaatggga gagatatgta cgtcgaccaa gagctcgaca ttaacagact
     9181 ctccgactat gatgtggatc acattgtccc tcaatctttc ctgaaggacg atagtattga
     9241 caacaaggta aagcaactgt gttttaatca atttcttgtc aggatatatg gattataact
     9301 taatttttga gaaatctgta gtatttggcg tgaaatgagt ttgctttttg gtttctcccg
     9361 tgttataggt ccttacgcgc tcagacaaga accgcggaaa atccgacaat gtacccagcg
     9421 aggaggttgt gaagaagatg aagaactatt ggaggcagct tttgaatgct aagctcataa
     9481 cccaacggaa attcgacaat ctcacgaagg cagaaagggg cggactgtct gagctcgaca
     9541 aagccggctt catcaagcgc cagttggttg aaactcgtca gattacgaaa catgtggccc
     9601 agatactcga ttcgcgtatg aatacgaagt atgatgagaa tgacaaactt atcagggagg
     9661 taaaggtaaa gtttccaact ttcctttacc atatcaaact aaagttcgaa actttttatt
     9721 tgatcaactt caaggccacc cgatctttct attcctgatt aatttgtgat gaatccatat
     9781 tgacttttga tggttacgca ggtgatcacc ctcaagagca aactggttag tgacttccgg
     9841 aaggacttcc agttttacaa ggttcgcgag atcaacaact accatcatgc ccatgacgcc
     9901 tacctgaacg ccgttgttgg cactgctctc atcaagaagt atccgaaact ggagtctgag
     9961 tttgtgtacg gggattacaa ggtgtacgac gttaggaaga tgatcgcgaa gtcagaacaa
    10021 gagatcggca aggctaccgc gaaatacttc ttttactcga atatcatgaa cttcttcaag
    10081 acagagatca ctctggcgaa tggtgaaatc cggaagaggc ctctgatcga gacaaatggc
    10141 gaaacaggtc tgtctttcct atttcatatg tttaatccta ggaatttgat caattgattg
    10201 tatgtatgtc gatcccaaga ctttcttgtt cacttatatc ttaactctct ctttgctgtt
    10261 tcttgcaggt gagattgtct gggataaggg cagggatttt gcgactgtgc gtaaggttct
    10321 cagcatgccc caagtcaaca tagtcaagaa aacggaggtt caaaccggtg gtttctccaa
    10381 ggagtccatt ctccctaagc gcaactccga caaactgatt gcgaggaaga aggattggga
    10441 tccgaagaaa tacggaggct ttgatagccc taccgtggca tacagcgtac tggtagtggc
    10501 caaggtggag aagggcaaga gcaagaaact gaaaagcgtc aaggaactgc ttggaattac
    10561 cataatggaa aggtcctcgt tcgagaagaa tccgatcgac ttcctcgagg ctaaaggtaa
    10621 aatattggat gccagacgat attctttctt ttgatttgta actttttcct gtcaaggtcg
    10681 ataaatttta ttttttttgg taaaaggtcg ataatttttt tttggagcca ttatgtaatt
    10741 ttcctaatta actgaaccaa aattatacaa accaggttac aaagaggtga agaaagacct
    10801 cattatcaaa ctgcccaagt attcgctttt cgaattggaa aatggcagaa aacgcatgct
    10861 ggcatctgcc ggagaactgc agaagggcaa cgagctggca ttgcccagta agtacgtcaa
    10921 cttcctgtac ttggcctcac actatgagaa gctgaagggg tcaccagagg acaacgagca
    10981 gaagcagttg tttgtcgagc agcacaagca ctatcttgat gagatcatag agcagatcag
    11041 cgaattttcc aagcgggtca ttcttgcaga cgctaacctc gataaggtaa ggacttctca
    11101 tgaatattag tggcagatta gtgttgttaa agtctttggt tagataatcg atgcctccta
    11161 attgtccatg ttttactggt tttctacaat taaaggtgct ttccgcgtac aacaagcaca
    11221 gagataagcc gataagggaa caagcggaaa acatcatcca cctgttcaca ctgaccaatc
    11281 tgggagcccc agcagccttt aagtacttcg ataccactat cgacagaaag cgctacacat
    11341 caaccaagga agtgttggac gctaccctta ttcaccaatc tattacaggg ctctatgaga
    11401 caaggataga tctgtcgcag ttgggtggtg actctagggc tgacccaaag aagaagcgta
    11461 aagtctgagc ttgctttcgt tcgtatcatc ggtttcgaca acgttcgtca agttcaatgc
    11521 atcagtttca ttgcgcacac accagaatcc tactgagttt gagtattatg gcattgggaa
    11581 aactgttttt cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt attcggtttt
    11641 cgctatcgaa ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat atggtccttt
    11701 tgttcattct caaattaata ttatttgttt tttctcttat ttgttgtgtg ttgaatttga
    11761 aattataaga gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat cgtggcctct
    11821 aatgaccgaa gttaatatga ggagtaaaac acttgtagtt gtaccattat gcttattcac
    11881 taggcaacaa atatattttc agacctagaa aagctgcaaa tgttactgaa tacaagtatg
    11941 tcctcttgtg ttttagacat ttatgaactt tcctttatgt aattttccag aatccttgtc
    12001 agattctaat cattgcttta taattatagt tatactcatg gatttgtagt tgagtatgaa
    12061 aatatttttt aatgcatttt atgacttgcc aattgattga caacgctaag cttgaattct
    12121 tgcttgtctt c
//
