LOCUS       sequence-377126-       10437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector-mediated delivery of artificial exons for protein 
            tagging in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Bialkowska A, Zhang XY, Reiser J
  TITLE     Improved tagging strategy for protein identification in mammalian 
            cells.
  JOURNAL   BMC Genomics. 2005 Sep 4;6:113. doi: 10.1186/1471-2164-6-113.
  PUBMED    16138932
REFERENCE   2  (bases 1 to 10437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2005-09-4"; volume: "6:113. doi"; pages: " 
            10.1186/1471-2164-6-113"
COMMENT     Sequence Label: pNL-5.1
FEATURES             Location/Qualifiers
     source          1..10437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     12..31
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(250..354)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     422..440
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(478..500)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     600..619
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     LTR             2312..2569
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      3196..3331
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    3609..3842
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4027..4071
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     complement(4325..4345)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4330..4659
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4510..4645
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4694..5488
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4694..5488
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4748..4767)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5358..5377
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    5786..5819
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5831..6226
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    6236..6269
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(6272..6274,6275..6985)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6272..6274,6275..6985)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6314..6335)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6575..6594)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6922..6943
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             7076..7256
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8896..8913)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9067..9655)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9147..9166)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9826..10437,1..180,181..249))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
       61 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      121 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      181 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      241 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      301 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      361 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
      421 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
      481 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
      541 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
      601 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
      661 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
      721 agggcgatcg gtgcgggcct cttcgctatt acgccagggg aggcagagat tgcagtaagc
      781 tgagatcgca gcactgcact ccagcctggg cgacagagta agactctgtc tcaaaaataa
      841 aataaataaa tcaatcagat attccaatct tttcctttat ttatttattt attttctatt
      901 ttggaaacac agtccttcct tattccagaa ttacacatat attctatttt tctttatatg
      961 ctccagtttt ttttagacct tcacctgaaa tgtgtgtata caaaatctag gccagtccag
     1021 cagagcctaa aggtaaaaaa taaaataata aaaaataaat aaaatctagc tcactccttc
     1081 acatcaaaat ggagatacag ctgttagcat taaataccaa ataacccatc ttgtcctcaa
     1141 taattttaag cgcctctctc caccacatct aactcctgtc aaaggcatgt gccccttccg
     1201 ggcgctctgc tgtgctgcca accaactggc atgtggactc tgcagggtcc ctaactgcca
     1261 agccccacag tgtgccctga ggctgcccct tccttctagc ggctgccccc actcggcttt
     1321 gctttcccta gtttcagtta cttgcgttca gccaaggtct gaaactaggt gcgcacagag
     1381 cggtaagact gcgagagaaa gagaccagct ttacaggggg tttatcacag tgcaccctga
     1441 cagtcgtcag cctcacaggg ggtttatcac attgcaccct gacagtcgtc agcctcacag
     1501 ggggtttatc acagtgcacc cttacaatca ttccatttga ttcacaattt ttttagtctc
     1561 tactgtgcct aacttgtaag ttaaatttga tcagaggtgt gttcccagag gggaaaacag
     1621 tatatacagg gttcagtact atcgcatttc aggcctccac ctgggtcttg gaatgtgtcc
     1681 cccgaggggt gatgactacc tcagttggat ctccacaggt cacagtgaca caagataacc
     1741 aagacacctc ccaaggctac cacaatgggc cgccctccac gtgcacatgg ccggaggaac
     1801 tgccatgtcg gaggtgcaag cacacctgcg catcagagtc cttggtgtgg agggagggac
     1861 cagcgcagct tccagccatc cacctgatga acagaaccta gggaaagccc cagttctact
     1921 tacaccagga aaggctggaa gggctaattt ggtcccaaaa aagacaagag atccttgatc
     1981 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     2041 ggatcagata tccactgacc tttggatggt gcttcaagtt agtaccagtt gaaccagagc
     2101 aagtagaaga ggccaatgaa ggagagaaca acagcttgtt acaccctatg agccagcatg
     2161 ggatggagga cccggaggga gaagtattag tgtggaagtt tgacagcctc ctagcatttc
     2221 gtcacatggc ccgagagctg catccggagt actacaaaga ctgctgacat cgagctttct
     2281 acaagggact ttccgctggg gactttccag ggaggtgtgg cctgggcggg actggggagt
     2341 ggcgagccct cagatgctac atataagcag ctgctttttg cctgtactgg gtctctctgg
     2401 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     2461 caataaagct tgccttgagt gctcaaagta gtgtgtgccc gtctgttgtg tgactctggt
     2521 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     2581 acagggactt gaaagcgaaa gtaaagccag aggagatctc tcgacgcagg actcggcttg
     2641 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     2701 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cggtattaag cgggggagaa
     2761 ttagataaat gggaaaaaat tcggttaagg ccagggggaa agaaacaata taaactaaaa
     2821 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg ccttttagag
     2881 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     2941 gaagaactta gatcattata taatacaata gcagtcctct attgtgtgca tcaaaggata
     3001 gatgtaaaag acaccaagga agccttagat aagatagagg aagagcaaaa caaaagtaag
     3061 aaaaaggcac agcaagcagc agctgacaca ggaaacaaca gccaggtcag ccaaaattac
     3121 cctatagtgc agaacctcca ggggcaaatg gtacatcagg ccatatcacc tagaacttta
     3181 aatatgactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3241 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3301 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaatgcat aaaagaaaag
     3361 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     3421 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     3481 acagcagaga tccacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     3541 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     3601 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     3661 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     3721 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     3781 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     3841 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     3901 agttggagta ataaatctct ggaacagatt tggaataaca tgacctggat ggagtgggac
     3961 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     4021 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     4081 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     4141 gtaggtttaa gaatagtttt tgctgtactt tctgtagtga atagagttag gcagggatat
     4201 tcaccattat cgtttcagac ccacctccca atcccgaggg gacccgacag gcccgaagga
     4261 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     4321 atgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     4381 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     4441 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4501 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4561 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     4621 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
     4681 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     4741 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     4801 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     4861 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     4921 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     4981 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     5041 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     5101 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     5161 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     5221 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     5281 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     5341 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     5401 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     5461 ctatcgcctt cttgacgagt tcttctgaag atcgatccct cgattagtta ttaccggact
     5521 cagatctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaacaa
     5581 tgctgcttgt gcctggctag aagcacaaga ggaggaagag gtgggttttc cagtcacacc
     5641 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     5701 agaaaagggg ggactggaag ggctaattca ctcccaaaga agacaagatc tcagatccct
     5761 aattcctctc ttctcctctc tccagataac ttcgtataat gtatgctata cgaagttatg
     5821 ggaattcctg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc
     5881 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag
     5941 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga
     6001 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc
     6061 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct
     6121 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt
     6181 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaatgac tgcagataac
     6241 ttcgtataat gtatgctata cgaagttatg ggtgagcaag ggcgaggagc tgttcaccgg
     6301 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     6361 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     6421 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     6481 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     6541 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     6601 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     6661 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     6721 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     6781 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     6841 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     6901 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     6961 tctcggcatg gacgagctgt acaagaaggt aagtagatct cgctcgacgg taccgcgggc
     7021 ccgggatcca ccggatctag atgctacata taagcagctg ctttttgcct gtactgggtc
     7081 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7141 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7201 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcaccca
     7261 ggaggtagag gttgcagtga gccaagatcg cgccactgca ttccagcctg ggcaagaaaa
     7321 caagactgtc taaaataata ataataagtt aagggtatta aatatattta tacatggagg
     7381 tcataaaaat atatatattt gggctgggcg cagtggctca cacctgcgcc cggccctttg
     7441 ggaggccgag gcaggtggat cacctgagtt tgggagttcc agaccagcct gaccaacatg
     7501 gagaaacccc ttctctgtgt atttttagta gattttattt tatgtgtatt ttattcacag
     7561 gtatttctgg aaaactgaaa ctgtttttcc tctactctga taccacaaga atcatcagca
     7621 cagaggaaga cttctgtgat caaatgtggt gggagaggga ggttttcacc agcacatgag
     7681 cagtcagttc tgccgcagac tcggcgggtg tccttcggtt cagttccaac accgcctgcc
     7741 tggagagagg tcagaccaca gggtgagggc tcagtcccca agacataaac acccaagaca
     7801 taaacaccca acaggtccac cccgcctgct gcccaggcag agccgattca ccaagacggg
     7861 aattaggata gagaaagagt aagtcacaca gagccggctg tgcgggagaa cggagttcta
     7921 ttatgactca aatcagtctc cccaagcatt cggggatcag agtttttaag gataacttag
     7981 tgtgtagggg gccagtgagt tggagatgaa agcgtaggga gtcgaaggtg tccttttgcg
     8041 ccgagtcagt tcctgggtgg gggccacaag atcggatgag ccagtttatc aatccggggg
     8101 tgccagctga tccatggagt gcagggtctg caaaatatct caagcactga ttgatcttag
     8161 gttttacaat agtgatgtta ccccaggaac aatttgggga aggtcagaat cttgtagcct
     8221 gtagctgcat gactcctaaa ccataatttc ttttttgttt tttttttttt atttttgaga
     8281 cagggtctca ctctgtcacc taggctggag tgcagtggtg caatcacagc tcactgcagc
     8341 ctcaacgtcg taagctcaag cgatcctccc acctcagcct gcctggtagc tgagactaca
     8401 agcgacgccc cagttaattt ttgtattttt ggtagaggca gcgttttgcc gtgtggccct
     8461 ggctggtctc gaactcctgg gctcaagtga tccagcctca gcctcccaaa gtgctgggac
     8521 aaccggggcc agtcactgca cctggcccta aaccataatt tctaatcttt tggctaattt
     8581 gttagtccta caaaggcagt ctagtcccca ggcaaaaagg gggtttgttt cgggaaaggg
     8641 ctgttactgt ctttgtttca aactataaac taagttcctc ctaaacttag ttcggcctac
     8701 acccaggaat gaacaaggag agcttggagg ttagaagcac gatggaattg gttaggtcag
     8761 atctctttca ctgtctgagt tataattttg caatggtggt tcaaagactg cccgcttctg
     8821 acaccagtcg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8881 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8941 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9001 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9061 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9121 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9181 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9241 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9301 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9361 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9421 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9481 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9541 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9601 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9661 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9721 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9781 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9841 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9901 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9961 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    10021 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    10081 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10141 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10201 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10261 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10321 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10381 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggc
//
