LOCUS       sequence-377147-       12035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of AAV2 Rep78-III in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12035)
  AUTHORS   Li P, Marino MP, Zou J, Argaw T, Morreale MT, Iaffaldano BJ, Reiser 
            J
  TITLE     Efficiency and Specificity of Targeted Integration Mediated by the 
            Adeno-Associated Virus Serotype 2 Rep 78 Protein.
  JOURNAL   Hum Gene Ther Methods. 2018 Jun;29(3):135-145. doi: 
            10.1089/hgtb.2018.052.
  PUBMED    29860898
REFERENCE   2  (bases 1 to 12035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hgtb.2018";
            journalName: "Hum Gene Ther Methods"; date: "2018-06"; volume: "29";
            issue: "3"; pages: "135-145"
COMMENT     Sequence Label: pNL(CMV)Rep78-III/CAG/WPRE-Delta-U3
FEATURES             Location/Qualifiers
     source          1..12035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     42..61
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     LTR             1754..2011
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      2638..2773
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    3051..3284
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3469..3513
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        3783..4084
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          4390..5406
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5330..5352
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5414..5433
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    7400..7988
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7453..7473)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7871..7882)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8063..8296
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9936..9953)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10107..10695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10187..10206)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10866..11657,11658..11726))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11489..11508
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11727..11831)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11899..11917
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11955..11977)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
       61 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
      121 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
      181 ctcttcgcta ttacgccagg ggaggcagag attgcagtaa gctgagatcg cagcactgca
      241 ctccagcctg ggcgacagag taagactctg tctcaaaaat aaaataaata aatcaatcag
      301 atattccaat cttttccttt atttatttat ttattttcta ttttggaaac acagtccttc
      361 cttattccag aattacacat atattctatt tttctttata tgctccagtt ttttttagac
      421 cttcacctga aatgtgtgta tacaaaatct aggccagtcc agcagagcct aaaggtaaaa
      481 aataaaataa taaaaaataa ataaaatcta gctcactcct tcacatcaaa atggagatac
      541 agctgttagc attaaatacc aaataaccca tcttgtcctc aataatttta agcgcctctc
      601 tccaccacat ctaactcctg tcaaaggcat gtgccccttc cgggcgctct gctgtgctgc
      661 caaccaactg gcatgtggac tctgcagggt ccctaactgc caagccccac agtgtgccct
      721 gaggctgccc cttccttcta gcggctgccc ccactcggct ttgctttccc tagtttcagt
      781 tacttgcgtt cagccaaggt ctgaaactag gtgcgcacag agcggtaaga ctgcgagaga
      841 aagagaccag ctttacaggg ggtttatcac agtgcaccct gacagtcgtc agcctcacag
      901 ggggtttatc acattgcacc ctgacagtcg tcagcctcac agggggttta tcacagtgca
      961 cccttacaat cattccattt gattcacaat ttttttagtc tctactgtgc ctaacttgta
     1021 agttaaattt gatcagaggt gtgttcccag aggggaaaac agtatataca gggttcagta
     1081 ctatcgcatt tcaggcctcc acctgggtct tggaatgtgt cccccgaggg gtgatgacta
     1141 cctcagttgg atctccacag gtcacagtga cacaagataa ccaagacacc tcccaaggct
     1201 accacaatgg gccgccctcc acgtgcacat ggccggagga actgccatgt cggaggtgca
     1261 agcacacctg cgcatcagag tccttggtgt ggagggaggg accagcgcag cttccagcca
     1321 tccacctgat gaacagaacc tagggaaagc cccagttcta cttacaccag gaaaggctgg
     1381 aagggctaat ttggtcccaa aaaagacaag agatccttga tctgtggatc taccacacac
     1441 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     1501 cctttggatg gtgcttcaag ttagtaccag ttgaaccaga gcaagtagaa gaggccaatg
     1561 aaggagagaa caacagcttg ttacacccta tgagccagca tgggatggag gacccggagg
     1621 gagaagtatt agtgtggaag tttgacagcc tcctagcatt tcgtcacatg gcccgagagc
     1681 tgcatccgga gtactacaaa gactgctgac atcgagcttt ctacaaggga ctttccgctg
     1741 gggactttcc agggaggtgt ggcctgggcg ggactgggga gtggcgagcc ctcagatgct
     1801 acatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     1861 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     1921 gtgctcaaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     1981 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     2041 aagtaaagcc agaggagatc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     2101 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     2161 agagagatgg gtgcgagagc gtcggtatta agcgggggag aattagataa atgggaaaaa
     2221 attcggttaa ggccaggggg aaagaaacaa tataaactaa aacatatagt atgggcaagc
     2281 agggagctag aacgattcgc agttaatcct ggccttttag agacatcaga aggctgtaga
     2341 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     2401 tataatacaa tagcagtcct ctattgtgtg catcaaagga tagatgtaaa agacaccaag
     2461 gaagccttag ataagataga ggaagagcaa aacaaaagta agaaaaaggc acagcaagca
     2521 gcagctgaca caggaaacaa cagccaggtc agccaaaatt accctatagt gcagaacctc
     2581 caggggcaaa tggtacatca ggccatatca cctagaactt taaatatgac tccgcccatc
     2641 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     2701 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     2761 ttttttggag gcctaggctt ttgcaaatgc ataaaagaaa aggggggatt ggggggtaca
     2821 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     2881 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacaat
     2941 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3001 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3061 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3121 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3181 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3241 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3301 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3361 ctggaacaga tttggaataa catgacctgg atggagtggg acagagaaat taacaattac
     3421 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3481 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3541 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3601 tttgctgtac tttctgtagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3661 acccacctcc caatcccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3721 gagagagaca gagacagatc cattcgatta gtgaacggat ctagttaact agatctagag
     3781 tccgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     3841 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     3901 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     3961 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     4021 tacatgacct tatgggactt tcctacttgg cagtacatct gtattagtca tcgctattac
     4081 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
     4141 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
     4201 gggggggggg gggggggggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc
     4261 gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     4321 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     4381 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     4441 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     4501 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
     4561 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
     4621 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
     4681 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     4741 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     4801 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     4861 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     4921 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     4981 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
     5041 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     5101 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     5161 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     5221 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     5281 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     5341 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     5401 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc
     5461 ctcgaagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccatg ccggggtttt
     5521 acgagattgt gattaaggtc cccagcgacc ttgacgagca tctgcccggc atttctgaca
     5581 gctttgtgaa ctgggtggcc gagaaggaat gggagttgcc gccagattct gacatggatc
     5641 tgaatctgat tgagcaggca cccctgaccg tggccgagaa gctgcagcgc gactttctga
     5701 cggaatggcg ccgtgtgagt aaggccccgg aggccctttt ctttgtgcaa tttgagaagg
     5761 gagagagcta ctttcatatg catgtgttgg tggagactac gggagtgaag agtatggtgc
     5821 tagggaggtt tctgtcgcaa ataagagaga agctgatcca gcggatatac cgtggcattg
     5881 agcccaccct tcccaattgg tttgccgtga ccaaaactcg taacggagca ggggggggaa
     5941 ataaagtcgt cgacgagtgc tatattccga actacctctt gcccaagacg cagcccgaat
     6001 tgcagtgggc ctggaccaac atggagcaat acctgtcagc gtgcctcaac ttgaccgaaa
     6061 gaaagagact cgtggcccag cacctgaccc atgtctcaca gacccaggaa cagaataagg
     6121 aaaaccaaaa cccaaatagc gacgcccccg tgatacggag caagacctca gccaggtaca
     6181 tggagctggt cgggtggctc gtggacaagg ggattacctc ggagaagcag tggatccagg
     6241 aggaccaggc ctcatacatc tccttcaatg cggcctccaa ctcgcggtcc caaatcaagg
     6301 ctgccttgga caatgcggga aagattatga gcctgactaa aaccgccccc gactacctgg
     6361 tgggccagca gcccgtggag gacatttcca gcaatcggat ttataaaatt ttggaactaa
     6421 acgggtacga tccccaatat gcggcttccg tctttctggg atgggccacg aaaaagttcg
     6481 gcaagaggaa caccatctgg ctgtttgggc ctgcaactac cgggaagacc aacatcgcgg
     6541 aggccatagc ccacactgtg cccttttacg gatgcgtgaa ttggactaac gaaaatttcc
     6601 cctttaatga ttgcgtggac aagatggtta tttggtggga ggaaggaaag atgactgcga
     6661 aagtggtgga atccgctaag gctatcttgg gggggtcgaa agttcgggtc gaccagaagt
     6721 gcaaatcgtc cgcgcagatt gaccccaccc ccgtgattgt gacgtcaaat actaatatgt
     6781 gtgcggtcat cgatggcaat agcaccactt tcgaacatca gcaacccctc caggatcgta
     6841 tgtttaagtt cgagttgact cggcggctgg accacgattt cggcaaagtg acgaaacagg
     6901 aggtgaagga cttctttaga tgggccaagg accacgtggt ggaggtcgag cacgagtttt
     6961 atgtgaagaa ggggggggcc aaaaagcgcc ctgcaccttc cgacgccgac atttccgagc
     7021 caaagagagt gcgtgagagt gtggcccaac cctccaccag tgatgccgag gcctccatta
     7081 attatgccga ccgctatcag aataaatgtt ctcgtcacgt gggcatgaat ctgatgctgt
     7141 ttccctgcag acaatgcgag agaatgaatc agaattcaaa tatctgcttc actcacggac
     7201 agaaagactg tttagagtgc tttcccgtgt cagaatctca acccgtttct gtcgtcaaaa
     7261 aggcgtatca gaaactgtgc tacattcatc atatcatggg aaaggtgcca gacgcttgca
     7321 ctgcctgcga tctggtcaat gtggatttgg atgactgcat ctttgaacaa taagcggccg
     7381 catcgataag cttgtcgaga atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7441 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7501 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7561 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7621 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7681 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7741 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     7801 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7861 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7921 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7981 ctccccgcct gggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     8041 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaaagaag acaagatctg
     8101 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8161 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8221 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8281 tggaaaatct ctagcaccca ggaggtagag gttgcagtga gccaagatcg cgccactgca
     8341 ttccagcctg ggcaagaaaa caagactgtc taaaataata ataataagtt aagggtatta
     8401 aatatattta tacatggagg tcataaaaat atatatattt gggctgggcg cagtggctca
     8461 cacctgcgcc cggccctttg ggaggccgag gcaggtggat cacctgagtt tgggagttcc
     8521 agaccagcct gaccaacatg gagaaacccc ttctctgtgt atttttagta gattttattt
     8581 tatgtgtatt ttattcacag gtatttctgg aaaactgaaa ctgtttttcc tctactctga
     8641 taccacaaga atcatcagca cagaggaaga cttctgtgat caaatgtggt gggagaggga
     8701 ggttttcacc agcacatgag cagtcagttc tgccgcagac tcggcgggtg tccttcggtt
     8761 cagttccaac accgcctgcc tggagagagg tcagaccaca gggtgagggc tcagtcccca
     8821 agacataaac acccaagaca taaacaccca acaggtccac cccgcctgct gcccaggcag
     8881 agccgattca ccaagacggg aattaggata gagaaagagt aagtcacaca gagccggctg
     8941 tgcgggagaa cggagttcta ttatgactca aatcagtctc cccaagcatt cggggatcag
     9001 agtttttaag gataacttag tgtgtagggg gccagtgagt tggagatgaa agcgtaggga
     9061 gtcgaaggtg tccttttgcg ccgagtcagt tcctgggtgg gggccacaag atcggatgag
     9121 ccagtttatc aatccggggg tgccagctga tccatggagt gcagggtctg caaaatatct
     9181 caagcactga ttgatcttag gttttacaat agtgatgtta ccccaggaac aatttgggga
     9241 aggtcagaat cttgtagcct gtagctgcat gactcctaaa ccataatttc ttttttgttt
     9301 tttttttttt atttttgaga cagggtctca ctctgtcacc taggctggag tgcagtggtg
     9361 caatcacagc tcactgcagc ctcaacgtcg taagctcaag cgatcctccc acctcagcct
     9421 gcctggtagc tgagactaca agcgacgccc cagttaattt ttgtattttt ggtagaggca
     9481 gcgttttgcc gtgtggccct ggctggtctc gaactcctgg gctcaagtga tccagcctca
     9541 gcctcccaaa gtgctgggac aaccggggcc agtcactgca cctggcccta aaccataatt
     9601 tctaatcttt tggctaattt gttagtccta caaaggcagt ctagtcccca ggcaaaaagg
     9661 gggtttgttt cgggaaaggg ctgttactgt ctttgtttca aactataaac taagttcctc
     9721 ctaaacttag ttcggcctac acccaggaat gaacaaggag agcttggagg ttagaagcac
     9781 gatggaattg gttaggtcag atctctttca ctgtctgagt tataattttg caatggtggt
     9841 tcaaagactg cccgcttctg acaccagtcg ctgcattaat gaatcggcca acgcgcgggg
     9901 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9961 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    10021 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    10081 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    10141 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    10201 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    10261 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    10321 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    10381 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10441 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    10501 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    10561 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    10621 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10681 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    10741 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    10801 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    10861 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    10921 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    10981 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    11041 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    11101 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    11161 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    11221 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    11281 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    11341 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    11401 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    11461 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    11521 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    11581 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    11641 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    11701 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    11761 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    11821 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    11881 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
    11941 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
    12001 gcggatgccg ggagcagaca agcccgtcag ggcgc
//
