LOCUS       sequence-377148-       15269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dCas9 fused to the SunTag scaffold.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15269)
  AUTHORS   Danziger O, Patel RS, DeGrace EJ, Rosen MR, Rosenberg BR
  TITLE     Inducible CRISPR activation screen for interferon-stimulated genes 
            identifies OAS1 as a SARS-CoV-2 restriction factor.
  JOURNAL   PLoS Pathog. 2022 Apr 14;18(4):e1010464. doi: 
            10.1371/journal.ppat.1010464. eCollection 2022 Apr.
  PUBMED    35421191
REFERENCE   2  (bases 1 to 15269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2022-04-14"; volume: "18(4):e1010464. doi"; pages: 
            " 10.1371/journal.ppat.1010464. eCollection 2022 Apr"
COMMENT     Sequence Label: pHR-PGK- dCas9-SunTag-P2A-HygR
FEATURES             Location/Qualifiers
     source          1..15269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             66..299
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(324..342)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(325..342)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(632..651)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(865..884)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     984..1006
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1044..1062)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1130..1234
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1235..1303,1304..2095)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1453..1472)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2266..2854
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2755..2774
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3008..3025
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3095..3115)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3100..3429
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3280..3415
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4563..4628
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4758..4778
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5203..5337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5240..5259)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5294..5313
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5505..6138
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6185..6310
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6807..7040
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7225..7269
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7554..7671
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7731..8230
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7766..7789)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8163..8182
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8286..8306
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      8355..8364
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8361..8387,8388..8390,8391..10877,10878..10880,
                     10881..12464)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8361..12464
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             12468..12494
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             12474..12500
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             12513..12533
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12540..12560
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12624..12680
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12696..12752
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12768..12824
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12840..12896
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12912..12968
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12984..13040
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13056..13112
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13128..13184
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13200..13256
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13272..13328
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             13410..13430
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13440..13496
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             13512..14531
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             13512..14531
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    14637..15225
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(14690..14710)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15108..15119)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(15228..15244)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15229..15245)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
       61 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
      121 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
      181 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
      241 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
      301 catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
      361 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
      421 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
      481 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
      541 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
      601 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
      661 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
      721 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
      781 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
      841 gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
      901 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
      961 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     1021 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     1081 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     1141 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1201 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     1261 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1321 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1381 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1441 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     1501 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1561 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     1621 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     1681 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1741 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1801 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1861 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1921 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     1981 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     2041 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     2101 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2161 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     2221 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2281 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2341 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2401 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2461 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2521 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2581 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2641 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2701 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2761 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2821 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2881 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2941 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3001 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     3061 ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga
     3121 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3181 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3241 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3301 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3361 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3421 ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta
     3481 tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt
     3541 tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc
     3601 cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat
     3661 gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg
     3721 cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt
     3781 attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt
     3841 aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg
     3901 gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc
     3961 atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa
     4021 gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt
     4081 cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt
     4141 acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga
     4201 aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa
     4261 tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta
     4321 tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga
     4381 cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac
     4441 ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg
     4501 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     4561 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     4621 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
     4681 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
     4741 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
     4801 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
     4861 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
     4921 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
     4981 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
     5041 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
     5101 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     5161 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     5221 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5281 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
     5341 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
     5401 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
     5461 taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca
     5521 ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     5581 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg
     5641 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac
     5701 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga
     5761 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta
     5821 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg
     5881 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
     5941 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6001 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6061 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6121 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     6181 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     6241 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     6301 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     6361 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     6421 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     6481 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     6541 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     6601 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg
     6661 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     6721 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     6781 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     6841 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     6901 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     6961 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     7021 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     7081 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     7141 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     7201 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     7261 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     7321 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     7381 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     7441 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     7501 cgattagtga acggatctcg acggtatcgc caaatggcag tattcatcca caattttaaa
     7561 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     7621 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     7681 tacagggaca gcagagatcc agtttggatc gataagcttg atatcgaatt gggtagggga
     7741 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     7801 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     7861 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     7921 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     7981 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     8041 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     8101 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     8161 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     8221 gggcctttcg aattctcacg cgtcaagtgg agcaaggcag gtggacagtg acgcgcgcca
     8281 ccatgcccaa gaagaagcgc aaggtgggac gcgtctgcag gatatcaagc ttgcggtacc
     8341 gcgggcccgg gatcgccacc atggacaaga agtacagcat cggcctggcc atcggcacca
     8401 actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg
     8461 tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggcgcc ctgctgttcg
     8521 acagcggaga aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca
     8581 gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg
     8641 acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg
     8701 agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca
     8761 ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga
     8821 tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc
     8881 tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc
     8941 agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg
     9001 ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga
     9061 agaatggcct gttcggcaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga
     9121 gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg
     9181 acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca
     9241 agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca
     9301 aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc
     9361 tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc
     9421 agagcaagaa cggctacgcc ggctacatcg atggcggagc cagccaggaa gagttctaca
     9481 agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga
     9541 acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga
     9601 tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga
     9661 aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc
     9721 ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca
     9781 ccccctggaa cttcgaggaa gtggtggaca agggcgccag cgcccagagc ttcatcgagc
     9841 ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc
     9901 tgtacgagta cttcaccgtg tacaacgagc tgaccaaagt gaaatacgtg accgagggaa
     9961 tgagaaagcc cgccttcctg agcggcgagc agaaaaaagc catcgtggac ctgctgttca
    10021 agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt
    10081 gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat
    10141 accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg
    10201 acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg
    10261 aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc
    10321 ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc
    10381 agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca
    10441 tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt
    10501 ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta
    10561 agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc
    10621 acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac
    10681 agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc
    10741 agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt
    10801 actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt
    10861 ccgactacga tgtggacgct atcgtgcctc agagctttct gaaggacgac tccatcgata
    10921 acaaagtgct gactcggagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag
    10981 aggtcgtgaa gaagatgaag aactactggc gccagctgct gaatgccaag ctgattaccc
    11041 agaggaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg
    11101 ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga
    11161 tcctggactc ccggatgaac actaagtacg acgagaacga caaactgatc cgggaagtga
    11221 aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca
    11281 aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg
    11341 gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca
    11401 aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg
    11461 ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca
    11521 acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaacaggc gagatcgtgt
    11581 gggataaggg ccgggacttt gccaccgtgc ggaaagtgct gtctatgccc caagtgaata
    11641 tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga
    11701 ggaacagcga caagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct
    11761 tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt
    11821 ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct
    11881 tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc
    11941 tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc
    12001 tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga
    12061 acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc
    12121 agaaacagct gtttgtggaa cagcacaaac actacctgga cgagatcatc gagcagatca
    12181 gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaggtg ctgagcgcct
    12241 acaacaagca cagagacaag cctatcagag agcaggccga gaatatcatc cacctgttta
    12301 ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga
    12361 agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg
    12421 gcctgtacga gacacggatc gacctgtctc agctgggagg cgacgcctat ccctatgacg
    12481 tgcccgatta tgccagcctg ggcagcggct cccccaagaa aaaacgcaag gtggaagatc
    12541 ctaagaaaaa gcggaaagtg gacggcattg gtagtgggag caacggcagc agcggatcca
    12601 acggtccgac tgacgccgcg gaagaagaac ttttgagcaa gaattatcat cttgagaacg
    12661 aagtggctcg tcttaagaaa ggttctggca gtggagaaga actgctttca aagaattacc
    12721 acctggaaaa tgaggtagct agactgaaaa aggggagcgg aagtggggag gagttgctga
    12781 gcaaaaatta tcatttggag aacgaagtag cacgactaaa gaaagggtcc ggatcgggtg
    12841 aggagttact ctcgaaaaat tatcatctcg aaaacgaagt ggctcggcta aaaaagggca
    12901 gtggttctgg agaagagcta ttatctaaaa actaccacct cgaaaatgag gtggcacgct
    12961 taaaaaaggg aagtggcagt ggtgaagagc tactatccaa gaattatcat cttgagaacg
    13021 aggtagcgcg tttgaagaag ggttccggct caggagagga actgctctcg aagaactatc
    13081 atcttgaaaa tgaggtcgct cgattaaaaa agggatcggg cagtggtgag gaactacttt
    13141 caaagaatta ccacctcgaa aacgaagtag ctcgattaaa gaaaggttca gggtcgggtg
    13201 aagaattact gagtaaaaat tatcatctgg aaaatgaggt agcgagacta aaaaagggga
    13261 gtggttctgg cgaggaattg ctatcgaaaa attatcatct tgagaacgaa gttgctaggc
    13321 tcaaaaaggg ctcaggctca ggcaccgcgg taaacatagg tggtggaacc ggtccgatgg
    13381 atctacagcg gccgcaaggt ggaggtggac ccaagaagaa gcgcaaggtg ggaagcggag
    13441 ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctatgg
    13501 atagatccgg aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
    13561 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
    13621 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
    13681 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
    13741 acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
    13801 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
    13861 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
    13921 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
    13981 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
    14041 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
    14101 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
    14161 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
    14221 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
    14281 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
    14341 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
    14401 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
    14461 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
    14521 caaaggaata acttgacttg cggccgcaac tcccacctgc aacatgcgtg actgactgag
    14581 gccgcgactc tagagtcgac ctgcaggcat gcaagcttga tatcaagctt atcgataatc
    14641 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
    14701 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
    14761 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
    14821 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
    14881 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
    14941 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
    15001 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
    15061 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
    15121 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
    15181 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
    15241 ctcgagggaa ttaattcgag ctcggtacc
//
