LOCUS       sequence-377163-        5951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses VSV-GS in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Zhang XY, Kutner RH, Bialkowska A, Marino MP, Klimstra WB, Reiser J
  TITLE     Cell-specific targeting of lentiviral vectors mediated by fusion 
            proteins derived from Sindbis virus, vesicular stomatitis virus, or 
            avian sarcoma/leukosis virus.
  JOURNAL   Retrovirology. 2010 Jan 25;7:3. doi: 10.1186/1742-4690-7-3.
  PUBMED    20100344
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Retrovirology."; date: "2010-01-25"; volume: "7:3. doi"; pages: " 
            10.1186/1742-4690-7-3"
COMMENT     Sequence Label: pUB6-VSV-GS
FEATURES             Location/Qualifiers
     source          1..5951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     152..171
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     405..422
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    539..560
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(575..580,581..598,599..605)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    613..629
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     618..640
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     637..653
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     637..653
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    702..823
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(713..732)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     767..786
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(981..1379)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(1398..1445)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1493..1822)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1507..1642
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1561..1580)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     1807..1827
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(1836..2264)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1946..1967)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2158..2177
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2310..2534
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2523..2540
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(2563..2580)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(2590..2631)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(2825..2857)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             complement(3098..3124)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3218..4429)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(3362..3381)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     promoter        4478..4582
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4583..4651,4652..5443)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4801..4820)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(5614..5951,1..251)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
       61 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      121 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      181 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      241 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      301 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      361 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      421 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      481 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      541 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      601 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
      661 acgccaagct ctagctagag gtcgacggta tacagacatg ataagataca ttgatgagtt
      721 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
      781 tattgcttta tttgtaacca ttataagctg caataaacaa gttggggtgg gcgaagaact
      841 ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga
      901 agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtagcacg tgtcagtcct
      961 gctcctcggc cacgaagtgc ttagccctcc cacacataac cagagggcag caattcacga
     1021 atcccaactg ccgtcggctg tccatcactg tccttcacta tggctttgat cccaggatgc
     1081 agatcgagaa gcacctgtcg gcaccgtccg caggggctca agatgcccct gttctcattt
     1141 ccgatcgcga cgatacaagt caggttgcca gctgccgcag cagcagcagt gcccagcacc
     1201 acgagttctg cacaaggtcc cccagtaaaa tgatatacat tgacaccagt gaagatgcgg
     1261 ccgtcgctag agagagctgc gctggcgacg ctgtagtctt cagagatggg gatgctgttg
     1321 attgtagccg ttgctctttc aatgagggtg gattcttctt gagacaaagg cttggccatg
     1381 gtttagttcc tcaccttgtc gtattatact atgccgatat actatgccga tgattaattg
     1441 tcaacacgtg ctgatcagat ccgaaaatgg atatacaagc tcccgggagc tttttgcaaa
     1501 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gcagaggcgg
     1561 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     1621 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     1681 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg
     1741 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     1801 ttccacaccc taactgacac acattccaca gaattaattc gcgttaaatt tttgttaaat
     1861 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     1921 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     1981 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     2041 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     2101 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     2161 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     2221 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtggggat accccctaga
     2281 gccccagctg gttctttccg cctcagaagc catagagccc accgcatccc cagcatgcct
     2341 gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga
     2401 atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa ggacagtggg
     2461 agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca
     2521 actagaaggc acagtcgagg ctgatcagcg ggtttaaact caatggtgat ggtgatgatg
     2581 accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac cttcgaaccg
     2641 cgggccctct agactcgagc ggccgccact gtgctggata tctgcagaat tccaccacac
     2701 tggactagtg gatccgagct ccataaaaat taaaaactca aatataattg aggcctcttt
     2761 gagcatggta tcacaagttg atttggtcca aacatgaaga atctgttgtg caggatttga
     2821 gtcacttgcc cagcctgttc atctcaatgt ctgtgtagat ctgcctcttc ttggtgtgct
     2881 tcagcttgat gcacaggtgg atgcccactc tcagcaccag aaacagccca atgatcaggc
     2941 caatgatgaa gaaaaagctg gcaatgctgc tcttccagct gctgaaccag ccctccacca
     3001 gttcaatggg gttcttgctc aggcctgtgt ccccaaagaa caggctctca tcatcaggca
     3061 gctggctggc agcatcctgg atgtgggggt gctcaaaggc atagtcaggc acatcatagg
     3121 ggtacttgca gttcacccca atgaacagga aggccaggta cagcaggcac ttcatagtgt
     3181 caaggaaaca gatcgatctc tgttggtacc aagcttcgtc taacaaaaaa gccaaaaacg
     3241 gccagaattt agcggacaat ttactagtct aacactgaaa attacatatt gacccaaatg
     3301 attacatttc aaaaggtgcc taaaaaactt cacaaaacac actcgccaac cccgagcgca
     3361 tagttcaaaa ccggagcttc agctacttaa gaagataggt acataaaacc gaccaaagaa
     3421 actgacgcct cacttatccc tcccctcacc agaggtccgg cgcctgtcga ttcaggagag
     3481 cctaccctag gcccgaaccc tgcgtcctgc gacggagaaa agcctaccgc acacctaccg
     3541 gcaggtggcc ccaccctgca ttataagcca acagaacggg tgacgtcacg acacgacgag
     3601 ggcgcgcgct cccaaaggta cgggtgcact gcccaacggc accgccataa ctgccgcccc
     3661 cgcaacagac gacaaaccga gttctccagt cagtgacaaa cttcacgtca gggtccccag
     3721 atggtgcccc agcccatctc acccgaataa gagctttccc gcattagcga aggcctcaag
     3781 accttgggtt cttgccgccc accatgcccc ccaccttgtt tcaacgacct cacagcccgc
     3841 ctcacaagcg tcttccattc aagactcggg aacagccgcc attttgctgc gctcccccca
     3901 acccccagtt cagggcaacc ttgctcgcgg acccagacta cagcccttgg cggtctctcc
     3961 acacgcttcc gtcccaccga gcggcccggc ggccacgaaa gccccggcca gcccagcagc
     4021 ccgctactca ccaagtgacg atcacagcga tccacaaaca agaaccgcga cccaaatccc
     4081 ggctgcgacg gaactagctg tgccacaccc ggcgcgtcct tatataatca tcggcgttca
     4141 ccgccccacg gagatccctc cgcagaatcg ccgagaaggg actacttttc ctcgcctgtt
     4201 ccgctctctg gaaagaaaac cagtgcccta gagtcaccca agtcccgtcc taaaatgtcc
     4261 ttctgctgat actggggttc taaggccgag tcttatgagc agcgggccgc tgtcctgagc
     4321 gtccgggcgg aaggatcagg acgctcgctg cgcccttcgt ctgacgtggc agcgctcgcc
     4381 gtgaggaggg gggcgcccgc gggaggcgcc aaaacccggc gcggaggcca gatctcccga
     4441 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4501 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4561 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4621 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4681 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4741 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4801 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4861 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4921 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4981 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5041 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5101 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     5161 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5221 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5281 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5341 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5401 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     5461 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5521 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5581 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5641 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5701 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5761 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5821 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5881 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5941 ttcgtgcaca c
//
