LOCUS       sequence-377166-        6379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses membrane-bound form of human SCF in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Zhang XY, Kutner RH, Bialkowska A, Marino MP, Klimstra WB, Reiser J
  TITLE     Cell-specific targeting of lentiviral vectors mediated by fusion 
            proteins derived from Sindbis virus, vesicular stomatitis virus, or 
            avian sarcoma/leukosis virus.
  JOURNAL   Retrovirology. 2010 Jan 25;7:3. doi: 10.1186/1742-4690-7-3.
  PUBMED    20100344
REFERENCE   2  (bases 1 to 6379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Retrovirology."; date: "2010-01-25"; volume: "7:3. doi"; pages: " 
            10.1186/1742-4690-7-3"
COMMENT     Sequence Label: pUB6-HuMGF
FEATURES             Location/Qualifiers
     source          1..6379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(73..864,865..933))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     696..715
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(934..1038)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1087..2298
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2135..2154
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3313..3354
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3364..3381
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3404..3421)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3410..3634
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3680..4108
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3767..3786)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3977..3998
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4117..4137)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4122..4451
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4302..4437
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4364..4383
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4499..4546
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4565..4963
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5121..5242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5158..5177)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5212..5231
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5291..5307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5291..5307)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5304..5326)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5315..5331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5339..5345,5346..5363,5364..5369))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5384..5405
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5522..5539)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5693..6281)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5773..5792)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
       61 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
      121 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
      181 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
      241 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
      301 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
      361 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
      421 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
      481 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
      541 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
      601 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
      661 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
      721 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
      781 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
      841 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
      901 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
      961 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     1021 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     1081 agatctggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc ctcacggcga
     1141 gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca
     1201 ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca
     1261 ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag
     1321 gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac
     1381 gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg cagccgggat
     1441 ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt agcgggctgc
     1501 tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag cgtgtggaga
     1561 gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg ggggttgggg
     1621 ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg
     1681 ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc aaggtcttga
     1741 ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc accatctggg
     1801 gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg ttgcgggggc
     1861 ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt
     1921 cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca cctgccggta
     1981 ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc
     2041 ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg tcagtttctt
     2101 tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg aactatgcgc
     2161 tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt
     2221 gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt ctggccgttt
     2281 ttggcttttt tgttagacga agcttgatat cgaattcctg cagcccgggc tggatcgcag
     2341 cgctgccttt ccttatgaag aagacacaaa cttggattct cacttgcatt tatcttcagc
     2401 tgctcctatt taatcctctc gtcaaaactg aagggatctg caggaatcgt gtgactaata
     2461 atgtaaaaga cgtcactaaa ttggtggcaa atcttccaaa agactacatg ataaccctca
     2521 aatatgtccc cgggatggat gttttgccaa gtcattgttg gataagcgag atggtagtac
     2581 aattgtcaga cagcttgact gatcttctgg acaagttttc aaatatttct gaaggcttga
     2641 gtaattattc catcatagac aaacttgtga atatagtgga tgaccttgtg gagtgcgtga
     2701 aagaaaactc atctaaggat ctaaaaaaat cattcaagag cccagaaccc aggctcttta
     2761 ctcctgaaga attctttaga atttttaata gatccattga tgccttcaag gactttgtag
     2821 tggcatctga aactagtgat tgtgtggttt cttcaacatt aagtcctgag aaagattcca
     2881 gagtcagtgt cacaaaacca tttatgttac cccctgttgc agccagctcc cttaggaatg
     2941 acagcagtag cagtaatagg aaggccaaaa atccccctgg agactccagc ctacactggg
     3001 cagccatggc attgccagca ttgttttctc ttataattgg ctttgctttt ggagccttat
     3061 actggaagaa gagacagcca agtcttacaa gggcagttga aaatatacaa attaatgaag
     3121 aggataatga gataagtatg ttgcaagaga aagagagaga gtttcaagaa gtgtaattgt
     3181 ggcttgtatc aacactgtta ctttcgtaca ttggctggta acattggggg atccactagt
     3241 ccagtgtggt ggaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc
     3301 ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
     3361 ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct
     3421 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     3481 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     3541 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     3601 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     3661 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     3721 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     3781 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     3841 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     3901 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3961 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4021 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     4081 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     4141 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4201 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4261 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     4321 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4381 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4441 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     4501 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     4561 aaccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
     4621 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
     4681 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
     4741 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
     4801 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
     4861 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
     4921 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagcacttc gtggccgagg
     4981 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     5041 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     5101 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     5161 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5221 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     5281 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     5341 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     5401 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     5461 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     5521 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5581 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     5641 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5701 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5761 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5821 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5881 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5941 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6001 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6061 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6121 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6181 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     6241 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6301 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     6361 tatcaaaaag gatcttcac
//
