LOCUS       sequence-377208-        8989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8989)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 8989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: DMGDH
FEATURES             Location/Qualifiers
     source          1..8989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1196..1215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1293..1317
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3946..3970)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4095..4136
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4591..4608)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4597..4821
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4954..4973)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5105..5152
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5160..6182
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5160..6182
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6312..6433
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6349..6368)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6403..6422
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6482..6498)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6482..6498)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6495..6517)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6506..6522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6530..6536,6537..6554,6555..6560))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6575..6596
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6713..6730)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6884..7472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6964..6983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7643..8434,8435..8503))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8266..8285
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8504..8608)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8683..8702)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac
     1261 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcagaagggc
     1321 cgtcaaggcc caccatgctc cgtcccggcg cgcagctgct gcggggcctc ctgctgcgga
     1381 gctgcccgct gcagggctcc cccgggcgcc cgcgctctgt ctgcggccgg gaaggagagg
     1441 aaaaaccacc cttatctgca gaaacacaat ggaaagacag agcagaaaca gtgataattg
     1501 gaggtggctg tgttggtgtg agtctggctt atcacctggc caaagcaggg atgaaagatg
     1561 tggtcctgct ggagaaatca gagctcacgg ctggatctac ctggcacgca gcaggtttaa
     1621 caacttactt tcatcctgga ataaacttga agaaaataca ttatgatagc atcaaacttt
     1681 atgagaaact ggaagaagaa actggtcagg tggtgggatt ccatcagcca ggtagtatca
     1741 gacttgctac cacccctgta agggtagatg aatttaaata tcaaatgact cggactggct
     1801 ggcatgcaac agaacagtat ctcattgaac ctgaaaaaat tcaagagatg ttccctttac
     1861 tcaacatgaa taaggtttta gctggattgt ataatcctgg agatggtcac attgatcctt
     1921 attctctaac tatggcactg gctgctgggg ctaggaaatg tggtgccctt ttaaaatatc
     1981 ctgcaccagt aacttctctg aaagccaggt cagatggaac atgggacgtt gaaacaccac
     2041 aggggtctat gagagcaaat agaattgtga atgctgcagg attttgggct cgtgaagtag
     2101 gtaaaatgat tggactagaa catcctctca ttccggttca acatcaatat gttgttacat
     2161 cgactatatc tgaagtgaaa gctttgaaac gagaactgcc tgtgctccgt gacctggaag
     2221 gatcatatta tctccgacag gaaagggatg ggcttttgtt tggtccatat gaaagtcaag
     2281 agaaaatgaa agttcaggac tcctgggtca ccaatggagt tcctccaggt tttggaaagg
     2341 aactctttga gtctgatcta gatcgaatca tggaacacat caaagctgcc atggaaatgg
     2401 ttcctgtctt gaaaaaggct gacatcatca atgttgtcaa tggtcctatc acgtattctc
     2461 ctgacattct gcctatggtg gggccccatc agggggtcag aaactactgg gtggctatag
     2521 gctttggata tggcataatc cacgctggtg gggtagggaa atatctcagt gactggatcc
     2581 tgcatggaga acctcctttt gatctgatag aattggatcc taatcgctat ggcaaatgga
     2641 caacaaccca gtacactgag gccaaagcaa gagaatcata tggattcaac aatattgttg
     2701 gttatcctaa agaagaacgg tttgctggga ggccgactca acgagtcagt gggctctatc
     2761 aaaggctgga gtctaagtgt tccatggggt tccatgctgg ctgggagcag ccgcactggt
     2821 tctacaaacc aggccaggac actcagtaca ggccaagttt tcgccgcaca aactggtttg
     2881 agcctgtggg ctcggagtat aaacaggtta tgcaaagagt agcggtaact gacctatcac
     2941 catttggcaa gtttaacatc aaaggccaag attccattag actactggac catctctttg
     3001 caaatgtcat tccaaaggtg ggttttacaa atataagtca catgttaaca cccaagggtc
     3061 gagtgtatgc tgagctgact gtttctcacc aatctcctgg ggagtttctt ttaattactg
     3121 gctctggatc agaacttcat gatcttagat ggattgaaga agaagcagtc aaaggtggat
     3181 atgatgttga aattaaaaac ataactgatg agcttggagt tcttggagtt gctgggccac
     3241 aggcaagaaa ggtccttcag aaactgacct ctgaagatct tagtgatgat gttttcaagt
     3301 ttcttcaaac caagtcctta aaggtttcca acattcctgt cactgctatt aggatatctt
     3361 atactggtga gctgggttgg gagctgtatc acagaagaga agattctgtg gcgctgtatg
     3421 acgctatcat gaatgcaggc caggaggagg gaatcgacaa ttttggaacc tatgccatga
     3481 atgccttacg cctggagaaa gccttcagag cctgggggtt agagatgaac tgtgatacaa
     3541 atcctttgga agctggactg gaatattttg tgaagttaaa taagccagca gacttcatag
     3601 gaaagcaagc actgaaacag attaaagcca aggggctgaa acgaagactg gtctgcctca
     3661 ccttggcaac ggatgatgtt gatccagagg gaaatgaaag catctggtac aatggcaagg
     3721 tggttggcaa cacgacatct ggaagctata gctacagcat ccagaagagt ctggctttcg
     3781 catatgtccc tgtacaacta agtgaagtgg gacagcaagt ggaagttgaa ctattaggca
     3841 aaaattaccc agcagtcatc atacaagaac ctttggtatt gaccgaacca accagaaacc
     3901 ggcttcagaa aaaaggtgga aaggacaaaa cttcaggcct catgggccca gctttcttgt
     3961 acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg
     4021 aaaccagcgg aggcggcgga agcgggggag gaggcagcgg cggaggcggc agcgggggcg
     4081 gcggaagcgc taccggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
     4141 ccggtatggc ttctaacttt actcagttcg ttctcgtcga caatggcgga actggcgacg
     4201 tgactgtcgc cccaagcaac ttcgctaacg gggtcgctga atggatcagc tctaactcgc
     4261 gatcacaggc ttacaaagta acctgtagcg ttcgtcagag ctctgcgcag aatcgcaaat
     4321 acaccatcaa agtcgaggtg cctaaagtgg caacccagac tgttggtggt gaagagcttc
     4381 ctgtagccgg atggagatct tacttaaata tggaactaac cattccaatt ttcgccacga
     4441 attccgactg cgagcttatt gttaaggcaa tgcaaggtct cctaaaagat ggaaacccga
     4501 ttccctcggc catcgcggcc aactccggca tctacggagg tggaggtgga tctcgtacgg
     4561 gttagtaatg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     4621 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     4681 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     4741 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     4801 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     4861 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4921 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4981 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5041 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5101 cctagaagtt cctattccga agttcctatt ctctagaaag tataggaact tccttggcca
     5161 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     5221 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     5281 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     5341 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     5401 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     5461 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     5521 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     5581 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     5641 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     5701 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     5761 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     5821 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     5881 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     5941 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     6001 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     6061 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     6121 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
     6181 agcacgtact acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     6241 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     6301 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6361 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6421 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     6481 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6541 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6601 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6661 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6721 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6781 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6841 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6901 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6961 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7021 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7081 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7141 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7201 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7261 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7321 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7381 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7441 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7501 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7561 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7621 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7681 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7741 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7801 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7861 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7921 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7981 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8041 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8101 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8161 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8221 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8281 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8341 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8401 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8461 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8521 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8581 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     8641 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     8701 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     8761 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     8821 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     8881 attattgact aggcttttgc aaaaagcttt gcaaagatgg ataaagtttt aaacagagag
     8941 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
