LOCUS       sequence-377227-       10141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 10141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: ZCCHC6
FEATURES             Location/Qualifiers
     source          1..10141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1196..1215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1293..1317
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5098..5122)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5247..5288
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5743..5760)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5749..5973
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6106..6125)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6257..6304
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6312..7334
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6312..7334
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7464..7585
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7501..7520)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7555..7574
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7634..7650)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7634..7650)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7647..7669)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7658..7674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7682..7688,7689..7706,7707..7712))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7727..7748
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7865..7882)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8036..8624)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8116..8135)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8795..9586,9587..9655))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9418..9437
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9656..9760)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9835..9854)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac
     1261 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gctggcacca
     1321 tgggagatac agcaaaacct tatttcgtga agcgcactaa agaccggggg actatggatg
     1381 atgatgactt cagaaggggt cacccccaac aagattattt aataatagat gaccatgcta
     1441 aaggccatgg cggtaaaatg gaaaagggcc ttcaaaaaaa gaagataaca ccagggaact
     1501 atgggaatac ccccagaaaa ggaccatgtg ctgtttccag caatccatat gcatttaaaa
     1561 acccaatcta cagtcaaccc gcttggatga atgacagcca caaagatcag agtaagagat
     1621 ggctgtctga tgaacatact ggtaattcag acaactggag agaattcaaa cctggaccta
     1681 gaattcctgt tataaaccga caaagaaaag actcctttca agaaaatgaa gatggttata
     1741 ggtggcaaga cacaagaggc tgcagaactg taagacgact gtttcataaa gacctaacaa
     1801 gcctagaaac cacgtcagaa atggaagcag gaagtcctga aaacaagaag cagaggtcca
     1861 gacctaggaa gccacggaag actagaaatg aggaaaatga gcaggatgga gacttggaag
     1921 gccctgtgat cgatgagtct gcactttcaa cgaaggagct gctaggctta cagcaggctg
     1981 aggagagact gaagagagac tgcattgaca ggctaaaaag gcgaccacga aactacccta
     2041 cagcaaagta cacctgcaga ctctgtgatg ttttaattga atccattgca tttgcccata
     2101 agcatatcaa ggaaaagagg cacaagaaaa acattaagga gaagcaagag gaagagttgc
     2161 tcactacgtt acccccacca acaccctccc agataaatgc agttggcatt gccattgaca
     2221 aagtggtaca ggaatttggc ttacacaatg agagcttgga acagaggcta gaaattaaac
     2281 gtatcatgga aaatgtgttc caacacaagt taccagattg ttccctaaga ttatatgggt
     2341 catcctgtag cagattgggt ttcaaaaatt cggatgtaaa cattgacatc cagtttccag
     2401 ccattattga aggattctca ttaagcaaac tagggaattt caaccttcaa gacattgaaa
     2461 aagatgttgt gatctgggaa catactgaca gtgctgcagg ggacacaggc ataacaaaag
     2521 aagaggcacc aagagaaacg ccgattaaaa ggggacaggt gtcattaata ttggatgtga
     2581 aacaccagcc ttcagtacca gttgggcagc tctgggtgga attgctgcgg ttctatgctt
     2641 tagaatttaa tttggctgat ttagtgataa gtattcgtgt caaagaattg gtatctcggg
     2701 aattgaagga ttggcccaaa aagcgcattg ccattgaaga tccctactct gttaaaagaa
     2761 atgtggcaag aaccctaaat agtcaacctg tgtttgaata tatacttcat tgtttaagga
     2821 caacatacaa gtattttgct cttccacaca aaattacaaa atccagcctt ctaaagcctc
     2881 tgaatgcaat tacatgtatt tcagaacatt ctaaagaagt aataaatcat catccagatg
     2941 tacaaacaaa agatgataag ctcaaaaact cagttttggc ccaaggtcct ggtgctacca
     3001 gttcagctgc aaatacctgt aaggtacagc cacttactct taaagagact gctgaaagtt
     3061 ttggaagccc accaaaagaa gaaatgggaa atgaacacat cagtgtccac cctgaaaact
     3121 cagactgtat ccaagcagat gttaactctg atgattacaa gggtgataaa gtataccatc
     3181 cagaaacagg aaggaaaaac gagaaagaga aagttggaag gaagggcaag catctgttga
     3241 ctgttgatca gaaacgtgga gagcatgttg tctgtggcag cacacgtaat aatgagtcag
     3301 agagcacttt ggatttagaa ggcttccaaa atcccacagc taaagagtgt gagggacttg
     3361 ccactttaga taacaaggct gatcttgatg gagaaagtac agaaggtact gaggaactag
     3421 aagactctct aaaccacttt acccactcag tacagggcca gacatcagaa atgattccct
     3481 ctgatgaaga ggaggaggac gacgaagaag aggaggagga agaagaacct aggctcacca
     3541 ttaaccaaag ggaagatgaa gatggcatgg ctaatgaaga tgagttagac aacacctaca
     3601 ctgggtcagg ggatgaggac gccctatctg aagaggatga tgagttaggc gaagctgcta
     3661 agtatgaaga cgtgaaagaa tgtggaaaac atgtagaaag agctctccta gtggaactta
     3721 ataaaataag tctcaaggaa gaaaatgtat gtgaagaaaa ttcacctgtg gatcagtctg
     3781 atttttttta tgaattcagt aaacttatct tcaccaaagg caagggattg gactgtgtca
     3841 gaactattga agaattagca agagtcctca gaaaacattc aggtctgaga aacatcttac
     3901 ctattacaac agcaaagatg ccaattgtga agttcttcca tttgagaagt ggtctggaag
     3961 tagatatcag tttgtataac acattggccc ttcataacac aaggctttta tctgcttatt
     4021 ccgccattga tcccagagtg aagtatttgt gctataccat gaaagtattt acaaagatgt
     4081 gtgatattgg tgatgcatct agaggcagct tatcatcgta tgcatatact cttatggtgc
     4141 tatattttct ccagcagagg aatccaccag tcattcctgt ccttcaagag atatacaaag
     4201 gtgaagagaa acctgaaata tttgttgatg gctggaatat ttattttttt gatcaaatag
     4261 atgaactgcc tacctattgg tcagaatgtg gaaaaaatac agaatctgtt gggcagttat
     4321 ggttgggcct tcttcgtttc tacacagagg aatttgattt taaagaacat gttattagca
     4381 tcaggagaaa aagtctgctt acaactttta agaaacagtg gacctcaaaa tacattgtta
     4441 ttgaagatcc ctttgatttg aatcataatc ttggagctgg attatcaagg aaaatgacaa
     4501 attttataat gaaggctttt atcaatggta gaagagtatt tggtattcct gtcaagggat
     4561 tttcaaagga ctacccctca aaaatggaat acttttttga tccagatgtg ttaactgaag
     4621 gagagctggc cccaaatgat agatgttgtc gaatttgtgg aaaaatcgga cacttcatga
     4681 aggactgtcc tatgaggaga aaagtaagac ggcggcgaga tcaggaagat gccctgaacc
     4741 aaagataccc tgagaacaag gaaaaaagaa gcaaagagga caaagaaatt cacaacaagt
     4801 acacagaaag ggaggtgtca acaaaagaag ataagcccat acagtgcaca cctcagaaag
     4861 ccaagccaat gcgggcagct gctgacctgg ggagggagaa gatcctcagg ccaccagtag
     4921 aaaaatggaa gagacaggat gacaaagact taagagaaaa acgttgtttt atttgtggaa
     4981 gagaagggca cattaaaaag gaatgcccac agtttaaagg ctcttcaggt agcctttcca
     5041 gtaaatacat gactcaggga aaagcctcag cgaagaggac ccagcaggaa tcaggcgacc
     5101 cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg agtctagagg
     5161 gcccgcggtt cgaaaccagc ggaggcggcg gaagcggggg aggaggcagc ggcggaggcg
     5221 gcagcggggg cggcggaagc gctaccggta agcctatccc taaccctctc ctcggtctcg
     5281 attctacgcg taccggtatg gcttctaact ttactcagtt cgttctcgtc gacaatggcg
     5341 gaactggcga cgtgactgtc gccccaagca acttcgctaa cggggtcgct gaatggatca
     5401 gctctaactc gcgatcacag gcttacaaag taacctgtag cgttcgtcag agctctgcgc
     5461 agaatcgcaa atacaccatc aaagtcgagg tgcctaaagt ggcaacccag actgttggtg
     5521 gtgaagagct tcctgtagcc ggatggagat cttacttaaa tatggaacta accattccaa
     5581 ttttcgccac gaattccgac tgcgagctta ttgttaaggc aatgcaaggt ctcctaaaag
     5641 atggaaaccc gattccctcg gccatcgcgg ccaactccgg catctacgga ggtggaggtg
     5701 gatctcgtac gggttagtaa tgagtttaaa cccgctgatc agcctcgact gtgccttcta
     5761 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     5821 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     5881 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     5941 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     6001 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     6061 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     6121 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     6181 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     6241 atggttcacg tacctagaag ttcctattcc gaagttccta ttctctagaa agtataggaa
     6301 cttccttggc caaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     6361 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     6421 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     6481 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     6541 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     6601 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     6661 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     6721 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     6781 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     6841 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     6901 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     6961 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     7021 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     7081 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     7141 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     7201 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     7261 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     7321 gggcaaagga atagcacgta ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     7381 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     7441 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     7501 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7561 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     7621 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7681 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7741 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7801 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7861 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7921 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7981 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8041 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8101 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8161 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8221 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8281 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8341 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8401 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8461 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8521 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8581 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8641 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8701 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8761 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8821 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8881 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8941 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9001 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9061 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9121 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9181 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9241 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9301 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9361 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9421 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9481 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9541 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9601 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9661 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9721 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9781 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
     9841 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     9901 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     9961 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
    10021 gcgttgacat tgattattga ctaggctttt gcaaaaagct ttgcaaagat ggataaagtt
    10081 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
    10141 t
//
