LOCUS       sequence-37724-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Banaszynski LA, Chen LC, Maynard-Smith LA, Ooi AG, Wandless TJ
  TITLE     A rapid, reversible, and tunable method to regulate protein function
            in living cells using synthetic small molecules.
  JOURNAL   Cell. 2006 Sep 8;126(5):995-1004.
  PUBMED    16959577
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2006-09-8"; volume: "126(5)"; pages: "995-1004"
COMMENT     Sequence Label: pBMN FKBP(DD)-YFP
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     307..323
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      378..387
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(384..386,387..707)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     primer_bind     complement(756..777)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gagatcttat atggggcacc cccgcccctt gtaaacttcc ctgaccctga catgacaaga
       61 gttactaaca gcccctctct ccaagctcac ttacaggctc tctacttagt ccagcacgaa
      121 gtctggagac ctctggcggc agcctaccaa gaacaactgg accgaccggt ggtacctcac
      181 ccttaccgag tcggcgacac agtgtgggtc cgccgacacc agactaagaa cctagaacct
      241 cgctggaaag gaccttacac agtcctgctg accaccccca ccgccctcaa agtagacggc
      301 atcgcagctt ggatacacgc cgcccacgtg aaggctgccg accccggggg tggaccatcc
      361 tctagactgc cggatccgcc accatgggag tgcaggtgga aaccatctcc ccaggagacg
      421 ggcgcacctt ccccaagcgc ggccagacct gtgtggtgca ctacaccggg atgcttgaag
      481 atggaaagaa agtcgattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca
      541 agcaggaggt gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag
      601 ccaaactgac tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc
      661 caccacatgc cactctcgtc ttcgatgtgg agcttctaaa accggaagaa ttcgtgagca
      721 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      781 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      841 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
//
