LOCUS       sequence-377247-        8837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses eGFP-p2A-HA-GPR151 (pArg95Ter variant) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Bielczyk-Maczynska E, Zhao M, Zushin PH, Schnurr TM, Kim HJ, Li J, 
            Nallagatla P, Sangwung P, Park CY, Cornn C, Stahl A, Svensson KJ, 
            Knowles JW
  TITLE     G protein-coupled receptor 151 regulates glucose metabolism and 
            hepatic gluconeogenesis.
  JOURNAL   Nat Commun. 2022 Dec 1;13(1):7408. doi: 10.1038/s41467-022-35069-9.
  PUBMED    36456565
REFERENCE   2  (bases 1 to 8837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-1"; volume: "13(1):7408. doi"; pages: " 
            10.1038/s41467-022-35069-9"
COMMENT     Sequence Label: CMV:eGFP-p2A-HA-GPR151(p.Arg95Ter)
FEATURES             Location/Qualifiers
     source          1..8837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    23..611
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(76..96)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(494..505)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             683..916
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    994..1115
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1031..1050)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1085..1104
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1155..1290
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1310..1329)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1311..1329)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1339..1356)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1339..1355)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1348..1370)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1497..1952
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1584..1603)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1794..1815
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1978..2082
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2083..2151,2152..2943)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2301..2320)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3114..3702
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3603..3622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3856..3873
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3990..4011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4026..4031,4032..4049,4050..4056)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4064..4080
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4069..4091
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4088..4104
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4088..4104
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4123..4143
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4125..4143
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4171..4397
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4398..4578
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4625..4750
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5243..5476
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5661..5705
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6013..6130
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6247..6550
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6551..6754
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6705..6725
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(6780..6782,6783..6785,6786..7496)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6780..6782,6783..6785,6786..7496)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6825..6846)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7086..7105)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7433..7454
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7506..7562
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7563..7589
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ggtgttaaat agttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
       61 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
      121 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
      181 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
      241 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
      301 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
      361 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
      421 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
      481 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
      541 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
      601 cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac
      661 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
      721 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
      781 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
      841 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
      901 tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa
      961 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     1021 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     1081 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     1141 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     1201 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     1261 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     1321 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     1381 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     1441 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     1501 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     1561 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     1621 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     1681 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     1741 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     1801 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     1861 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     1921 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     1981 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2041 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2101 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2161 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     2221 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     2281 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     2341 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     2401 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     2461 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     2521 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2581 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2641 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2701 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2761 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2821 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2881 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2941 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     3001 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3061 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3121 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3181 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3241 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3301 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3361 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3421 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3481 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3541 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3601 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3661 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3721 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3781 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3841 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3901 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3961 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     4021 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     4081 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     4141 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     4201 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     4261 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     4321 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     4381 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     4441 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     4501 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     4561 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     4621 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     4681 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     4741 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     4801 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     4861 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     4921 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     4981 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     5041 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     5101 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     5161 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     5221 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     5281 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     5341 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     5401 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     5461 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     5521 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     5581 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     5641 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     5701 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     5761 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     5821 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg agagcgctgc
     5881 cgtctggacc gatggctgtg tggaagtgct cgccgatagt ggaaacagac gccccagcac
     5941 tcgtcctagg gcaaaggatc tgcagtaatg agaattctag atcttgagac aaatggcagt
     6001 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     6061 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     6121 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg
     6181 ggcccggggt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     6241 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     6301 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     6361 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     6421 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     6481 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     6541 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     6601 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     6661 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     6721 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactgtcg ggatccacca
     6781 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     6841 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     6901 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     6961 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     7021 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     7081 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     7141 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     7201 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     7261 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     7321 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     7381 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     7441 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggaa
     7501 gcggagctac taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac
     7561 cttacccata tgacgttcca gattacgcta tgctggcagc tgcctttgca gactctaact
     7621 ccagcagcat gaatgtgtcc tttgctcacc tccactttgc cggagggtac ctgccctctg
     7681 attcccagga ctggagaacc atcatcccgg ctctcttggt ggctgtctgc ctggtgggct
     7741 tcgtgggaaa cctgtgtgtg attggcatcc tccttcacaa tgcttggaaa ggaaagccat
     7801 ccatgatcca ctccctgatt ctgaatctca gcctggctga tctctccctc ctgctgtttt
     7861 ctgcacctat ctgagctacg gcgtactcca aaagtgtttg ggatctaggc tggtttgtct
     7921 gcaagtcctc tgactggttt atccacacat gcatggcagc caagagcctg acaatcgttg
     7981 tggtggccaa agtatgcttc atgtatgcaa gtgacccagc caagcaagtg agtatccaca
     8041 actacaccat ctggtcagtg ctggtggcca tctggactgt ggctagcctg ttacccctgc
     8101 cggaatggtt ctttagcacc atcaggcatc atgaaggtgt ggaaatgtgc ctcgtggatg
     8161 taccagctgt ggctgaagag tttatgtcga tgtttggtaa gctctaccca ctcctggcat
     8221 ttggccttcc attatttttt gccagctttt atttctggag agcttatgac caatgtaaaa
     8281 aacgaggaac taagactcaa aatcttagaa accagatacg ctcaaagcaa gtcacagtga
     8341 tgctgctgag cattgccatc atctctgctc tcttgtggct ccccgaatgg gtagcttggc
     8401 tgtgggtatg gcatctgaag gctgcaggcc cggccccacc acaaggtttc atagccctgt
     8461 ctcaagtctt gatgttttcc atctcttcag caaatcctct catttttctt gtgatgtcgg
     8521 aagagttcag ggaaggcttg aaaggtgtat ggaaatggat gataaccaaa aaacctccaa
     8581 ctgtctcaga gtctcaggaa acaccagctg gcaactcaga gggtcttcct gacaaggttc
     8641 catctccaga atccccagca tccataccag aaaaagagaa acccagctct ccctcctctg
     8701 gcaaagggaa aactgagaag gcagagattc ccatccttcc tgacgtagag cagttttggc
     8761 atgagaggga cacagtccct tctgtacagg acaatgaccc tatcccctgg gaacatgaag
     8821 atcaagagac aggggaa
//
