LOCUS       sequence-377248-        8837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses eGFP-p2A-HA-GPR151 (wild-type variant) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Bielczyk-Maczynska E, Zhao M, Zushin PH, Schnurr TM, Kim HJ, Li J, 
            Nallagatla P, Sangwung P, Park CY, Cornn C, Stahl A, Svensson KJ, 
            Knowles JW
  TITLE     G protein-coupled receptor 151 regulates glucose metabolism and 
            hepatic gluconeogenesis.
  JOURNAL   Nat Commun. 2022 Dec 1;13(1):7408. doi: 10.1038/s41467-022-35069-9.
  PUBMED    36456565
REFERENCE   2  (bases 1 to 8837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-1"; volume: "13(1):7408. doi"; pages: " 
            10.1038/s41467-022-35069-9"
COMMENT     Sequence Label: CMV:eGFP-p2A-HA-GPR151
FEATURES             Location/Qualifiers
     source          1..8837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    30..618
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(83..103)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(501..512)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             690..923
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1001..1122
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1038..1057)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1092..1111
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1162..1297
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1317..1336)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1318..1336)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1346..1363)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1346..1362)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1355..1377)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1504..1959
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1591..1610)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1801..1822
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1985..2089
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2090..2158,2159..2950)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2308..2327)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3121..3709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3610..3629
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3863..3880
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3997..4018
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4033..4038,4039..4056,4057..4063)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4071..4087
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4076..4098
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4095..4111
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4095..4111
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4130..4150
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4132..4150
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4178..4404
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4405..4585
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4632..4757
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5250..5483
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5668..5712
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6020..6137
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6254..6557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6558..6761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6712..6732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(6787..6789,6790..6792,6793..7503)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6787..6789,6790..6792,6793..7503)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6832..6853)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7093..7112)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7440..7461
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7513..7569
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7570..7596
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aggggaaggt gttaaatagt taagtcgaca atcaacctct ggattacaaa atttgtgaaa
       61 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
      121 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
      181 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
      241 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
      301 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
      361 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
      421 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
      481 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
      541 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
      601 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
      661 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
      721 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
      781 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      841 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      901 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
      961 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     1021 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     1081 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     1141 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     1201 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     1261 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     1321 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     1381 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     1441 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     1501 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     1561 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     1621 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     1681 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     1741 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     1801 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     1861 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     1921 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     1981 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2041 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2101 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2161 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2221 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     2281 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     2341 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     2401 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     2461 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     2521 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     2581 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     2641 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     2701 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     2761 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2821 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2881 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2941 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3001 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3061 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3121 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3181 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3241 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3301 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3361 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3421 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3481 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3541 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3601 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3661 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3721 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3781 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3841 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     3901 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     3961 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     4021 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     4081 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     4141 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     4201 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     4261 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     4321 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     4381 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     4441 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     4501 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     4561 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     4621 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     4681 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     4741 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     4801 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     4861 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     4921 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     4981 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     5041 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     5101 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     5161 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     5221 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     5281 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     5341 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     5401 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     5461 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     5521 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     5581 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     5641 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     5701 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     5761 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     5821 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgaga
     5881 gcgctgccgt ctggaccgat ggctgtgtgg aagtgctcgc cgatagtgga aacagacgcc
     5941 ccagcactcg tcctagggca aaggatctgc agtaatgaga attctagatc ttgagacaaa
     6001 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     6061 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     6121 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
     6181 tcgagggggc ccggggttat taatagtaat caattacggg gtcattagtt catagcccat
     6241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     6301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     6361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     6421 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     6481 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     6541 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     6601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     6661 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     6721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actgtcggga
     6781 tccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     6841 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     6901 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     6961 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     7021 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     7081 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     7141 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     7201 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     7261 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     7321 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     7381 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     7441 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     7501 aagggaagcg gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac
     7561 cctggacctt acccatatga cgttccagat tacgctatgc tggcagctgc ctttgcagac
     7621 tctaactcca gcagcatgaa tgtgtccttt gctcacctcc actttgccgg agggtacctg
     7681 ccctctgatt cccaggactg gagaaccatc atcccggctc tcttggtggc tgtctgcctg
     7741 gtgggcttcg tgggaaacct gtgtgtgatt ggcatcctcc ttcacaatgc ttggaaagga
     7801 aagccatcca tgatccactc cctgattctg aatctcagcc tggctgatct ctccctcctg
     7861 ctgttttctg cacctatccg agctacggcg tactccaaaa gtgtttggga tctaggctgg
     7921 tttgtctgca agtcctctga ctggtttatc cacacatgca tggcagccaa gagcctgaca
     7981 atcgttgtgg tggccaaagt atgcttcatg tatgcaagtg acccagccaa gcaagtgagt
     8041 atccacaact acaccatctg gtcagtgctg gtggccatct ggactgtggc tagcctgtta
     8101 cccctgccgg aatggttctt tagcaccatc aggcatcatg aaggtgtgga aatgtgcctc
     8161 gtggatgtac cagctgtggc tgaagagttt atgtcgatgt ttggtaagct ctacccactc
     8221 ctggcatttg gccttccatt attttttgcc agcttttatt tctggagagc ttatgaccaa
     8281 tgtaaaaaac gaggaactaa gactcaaaat cttagaaacc agatacgctc aaagcaagtc
     8341 acagtgatgc tgctgagcat tgccatcatc tctgctctct tgtggctccc cgaatgggta
     8401 gcttggctgt gggtatggca tctgaaggct gcaggcccgg ccccaccaca aggtttcata
     8461 gccctgtctc aagtcttgat gttttccatc tcttcagcaa atcctctcat ttttcttgtg
     8521 atgtcggaag agttcaggga aggcttgaaa ggtgtatgga aatggatgat aaccaaaaaa
     8581 cctccaactg tctcagagtc tcaggaaaca ccagctggca actcagaggg tcttcctgac
     8641 aaggttccat ctccagaatc cccagcatcc ataccagaaa aagagaaacc cagctctccc
     8701 tcctctggca aagggaaaac tgagaaggca gagattccca tccttcctga cgtagagcag
     8761 ttttggcatg agagggacac agtcccttct gtacaggaca atgaccctat cccctgggaa
     8821 catgaagatc aagagac
//
