LOCUS       sequence-377272-        5222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Regulates bacterial expression of target gene in 
            blue-light-repressed manner. Based on Nakamurella multipartita PAL..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Ranzani AT, Wehrmann M, Kaiser J, Juraschitz M, Weber AM, 
            Pietruschka G, Gerken U, Mayer G, Moglich A
  TITLE     Light-Dependent Control of Bacterial Expression at the mRNA Level.
  JOURNAL   ACS Synth Biol. 2022 Oct 21;11(10):3482-3492. doi: 
            10.1021/acssynbio.2c00365. Epub 2022 Sep 21.
  PUBMED    36129831
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00365"; journalName: "ACS Synth Biol"; date: 
            "2022-10-21- 21"; volume: "11"; issue: "10"; pages: "3482-3492"
COMMENT     Sequence Label: pCrepusculo
FEATURES             Location/Qualifiers
     source          1..5222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     213..232
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1453..1482
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(1535..1553)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1549..1596
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1650..1667)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             1823..1840
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1850..1867
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     terminator      2047..2133
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2225..2252
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(2453..3244)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(3245..3336)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3384..4122)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(4323..5201)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
       61 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
      121 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
      181 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
      241 agcggatcct acctttttaa aaaattcatt tgctaaacgc ttcaaattct cgtataatta
      301 cttttctcca tacccgtttt ttgggctaac ccgccgaaac ctaaggaaat acatagtatg
      361 aaagttaatc gtccggcaga acgtgccagc tttggtagct ttgttctgga tgcaggtagc
      421 gcacgttttg ttggtagtga tgaactggca ctggttctgg gttttgcacc gggtgatgtt
      481 gttctgacac cggcagttgt tctggcacat ctgcatcctg atgatcgtct ggaatggcag
      541 gcaggtctgc agcgttgtct ggcaaccggt cgtccggtgg ttgttaatca tctgctgctg
      601 accgcagaag cagaaccgcg tccggcaatg acaaccctga ccgcactgac cgaacaggat
      661 cgtgtgcgtg cagttaccgg tgttattacc gatctgagcg atcgtgttcg tcgtgcaacc
      721 gaagcagaaa ttcgtcaggc agttcgtgca gcagcagcaa cccgtagcga aattgatcag
      781 gcaaaaggta ttgttatggc agcctttgat gttgatgcag atcaggcatt tgcactgctg
      841 aaatggcata gcagccagag caatcgtaaa ctgcgtgatc tggccaccgg tatgattgaa
      901 ggtctggcag cagccaatag cgcactgccg ctgcgtcgtc gtctgagcac cgtttttacc
      961 gatatgggtt gtccggcacc gagcaccaaa ggttggaccg ttccggtgac cgatattggt
     1021 ctgcctccga ccagcggtct gattccgaca gcactgctgc ctggtattct gacccgtgca
     1081 gcacatgatg caagcgttgc aattaccgtt gcagatgtta ccgcaccgga tcagcctctg
     1141 gtttatgcaa atcctgcatt tgaacgtctg accggttatg cagcagccga agttctgggt
     1201 cgtaattgtc gttttctgca ggcagaaagc ggtgatccgc atgaacgtag cgcaattcgt
     1261 agcgccattg caaatggtga tgcagtgacc acactgattc gtaattttcg tcaggatggt
     1321 catgcctttt ggaacgaatt tcatctgagt ccggttcgta atggtgcagg tcgtgttacc
     1381 cattacattg gttatcagct ggatgtgacc gaacgtgttg aacgtgatca gcagctggaa
     1441 cagctggcaa gcgaacagaa actgatttct gaagaagatc tgtaagatcc ggctgctaac
     1501 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
     1561 cttggggcct ctaaacgggt cttgaggggt tttttgctcg gccgcactcg agagcttggc
     1621 tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
     1681 ggtctgataa aacagaattt gccggtgttg acataaatac cactggcggt gatactgagc
     1741 acatcagcag gcccctctag aaataatttt gtttaacttt aaatcttcag cagcgaggag
     1801 gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg
     1861 cggcagccat atgggatccg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc
     1921 atgagatccc tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag
     1981 tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc
     2041 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2101 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg
     2161 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt
     2221 aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt
     2281 tttctaaata cattcaaata tgtatccgct catggacact gcttccggta gtcaataaac
     2341 cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa ccgacgaccg
     2401 ggtcatcgtg gccggatctt gcggcccctc ggcttgaacg aattgttaga cattatttgc
     2461 cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac tgatctgcgc
     2521 gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg
     2581 gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga
     2641 ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat
     2701 cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata
     2761 gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc
     2821 tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga
     2881 agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg
     2941 gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa
     3001 tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg
     3061 ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca
     3121 ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc
     3181 gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc
     3241 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3301 gatacatatt tgaatgtatt tagaaaaata aacaaatagc tagctcactc ggtcgctacg
     3361 ctccgggcgt gagactgcgg cgggcgctgc ggacacatac aaagttaccc acagattccg
     3421 tggataagca ggggactaac atgtgaggca aaacagcagg gccgcgccgg tggcgttttt
     3481 ccataggctc cgccctcctg ccagagttca cataaacaga cgcttttccg gtgcatctgt
     3541 gggagccgtg aggctcaacc atgaatctga cagtacgggc gaaacccgac aggacttaaa
     3601 gatccccacc gtttccggcg ggtcgctccc tcttgcgctc tcctgttccg accctgccgt
     3661 ttaccggata cctgttccgc ctttctccct tacgggaagt gtggcgcttt ctcatagctc
     3721 acacactggt atctcggctc ggtgtaggtc gttcgctcca agctgggctg taagcaagaa
     3781 ctccccgttc agcccgactg ctgcgcctta tccggtaact gttcacttga gtccaacccg
     3841 gaaaagcacg gtaaaacgcc actggcagca gccattggta actgggagtt cgcagaggat
     3901 ttgtttagct aaacacgcgg ttgctcttga agtgtgcgcc aaagtccggc tacactggaa
     3961 ggacagattt ggttgctgtg ctctgcgaaa gccagttacc acggttaagc agttccccaa
     4021 ctgacttaac cttcgatcaa accacctccc caggtggttt tttcgtttac agggcaaaag
     4081 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac tgaaccgctc
     4141 tagatttcag tgcaatttat ctcttcaaat gtagcacctg aagtcagccc catacgatat
     4201 aagttgtaat tctcatgtta gtcagccccg catgcataat gtgcctgtca aatggacgaa
     4261 gcagggattc tgcaaaccct atgctactcc gtcaagccgt caattgtctg attcgttacc
     4321 aattatgaca acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg
     4381 ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa
     4441 ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc
     4501 tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa
     4561 agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa
     4621 ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg attatccatc
     4681 ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga
     4741 tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca
     4801 aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc cgtattggca
     4861 aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac
     4921 tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc tcctggcggg
     4981 aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt caccaccccc
     5041 tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa
     5101 aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac
     5161 gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat actcccgcca
     5221 tt
//
