LOCUS       sequence-377309-        4557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Backbone for transcriptional reporter system in Fusobacterium 
            nucleatum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Ponath F, Zhu Y, Cosi V, Vogel J
  TITLE     Expanding the genetic toolkit helps dissect a global stress response
            in the early-branching species Fusobacterium nucleatum.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Oct 4;119(40):e2201460119. doi: 
            10.1073/pnas.2201460119. Epub 2022 Sep 26.
  PUBMED    36161895
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-10-4"; volume: "119(40):e2201460119. 
            doi"; pages: " 10.1073/pnas.2201460119. Epub 2022 Sep 26"
COMMENT     Sequence Label: pVoPo-01
FEATURES             Location/Qualifiers
     source          1..4557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1802..1810
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(3326..3337)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     terminator      3916..3950
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      join(4048..4557,1..79)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4537..4556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
       61 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggctctcga
      121 gtagatacag agtactagtt taataattta aggaggagaa taatggtatc taaaggagaa
      181 gaagataata tagctataat taaagaattt atgagattta aagttcatat ggaaggatct
      241 gttaatggac atgaatttga aatagaagga gaaggagaag gaagacctta tgaaggtact
      301 caaacagcta aattaaaagt aactaaaggt ggtccattac catttgcatg ggatatatta
      361 agtcctcaat ttatgtatgg atctaaagca tatgttaaac atcctgctga tatacctgat
      421 tatttaaaat tatcatttcc agaaggtttt aaatgggaaa gagttatgaa ttttgaagat
      481 ggtggagtag ttactgtaac acaagatagt tcattacaag atggagattt tatatataaa
      541 gttaaattaa gaggtactaa ttttccatct gatggaccag taatgcaaaa gaaaactatg
      601 ggatgggaag catcatctga aagaatgtat ccagaagatg gagcattaaa aggtgaaatt
      661 aaacaaagat taaaattaaa agatggaggt cattatgatg ctgaagttaa gactacatat
      721 aaagctaaga aaccagttca attaccaggt gcatataatg ttaatattaa attagatata
      781 acttctcata atgaagatta tactatagtt gaacaatatg aaagagcaga aggaagacat
      841 tcaacaggag gtatggatga attatataaa taaggatcct ataagtttta ataaaacttt
      901 aaatagaaaa aggcttctct catgagaagt cttttttatt taaaataaat ataaaataaa
      961 atagaggcta taaatagcct ctattttatg tgagaaatcc ctaaataaaa agatgccagt
     1021 gtgctggaat tcgcccttag gctgcgctag ggacctcttt agctcccttt tgctggcgaa
     1081 ttcttatgga ttttagggta acaaaaaaca ccgtatttct acgatgtttt tgcttaaata
     1141 cttgttttta gttacagaca aacctgaagt taactattta tcaattcctg caattcgttt
     1201 acaaaacggc aaatgtgaaa tccgtcacat actgcgtgat gaacttgaat tgccaaagga
     1261 agtataattt tgttatcttc tttataatat ttccccatag taaaaatagg aatcaaataa
     1321 tcatatcctt tctgcaaatt cagattaaag ccatcgaagg ttgaccacgg tatcatagat
     1381 acattaaaaa tgttttccgg agcatttggc tttccttcca ttctatgatt gtttccatac
     1441 cgttgcgtat cactttcata atctgctaaa aatgatttaa agtcagactt acactcagtc
     1501 caaaggctgg aaaatgtttc agtatcattg tgaaatattg tatagcttgg tatcatctca
     1561 tcatatatcc ccaattcacc atcttgattg attgccgtcc taaactctga atggcggttt
     1621 acaatcattg caatataata aagcattgca ggatatagtt tcattccctt ttcctttatt
     1681 tgtgtgatat ccactttaac ggtcatgctg taggtacaag gtacacttgc aaagtagtgg
     1741 tcaaaatact cttttctgtt ccaactattt ttatcaattt tttcaaatac catttaaaat
     1801 tacctcctta aaatatactg ctttaattgt ataattttag ctaattattg tcaagtattt
     1861 ttaaagttat gtaaattttt tacttatatt ttatcgatcg ttctagatat tcttttttaa
     1921 acttttcaaa tgcttcaaat tctacatctg caacttctac atcttttcca gtatttccaa
     1981 acttcacaca atttttgtaa acatttctta atttttccaa gtattctatg tcttcctttt
     2041 cttctttaac atactcttgt aactgtgtca tcaaattagt tttgtcttcc acattccccc
     2101 tttttcttgt aattgcctta tttaattctt caaatcttat tgttttcatt ttattgtacc
     2161 tccaattttt ttatatatct atgttaaaaa acatattgac aattgattaa aacaatgata
     2221 ttatggtcgg ttgagctcat tgagctgagt gcagactttc ccccttgtaa aaaggggaaa
     2281 aaacgattga taatgataca atggtcgtat atcgctatgt tcgcatcgtt taacttactt
     2341 tatcgcatat ttaattatat gccagtgtta gccctgccca acacctagag ttttagctta
     2401 ctttctaaat tataacccta ataaagtacc cactcagagc acctccaaga cttttaaagg
     2461 tagcctcgca ccttgctaca attcataata ccaccattac ggtgctatac cgtttcgagc
     2521 agaacggcta tacatacgaa tttatcttat gtataaaatt tgttacaaat ataatagtaa
     2581 gtttttttta tataaaaaag cagatcgctt ttattttttt tgcaacctgc ttcacaactc
     2641 atttttttac tttagtaaat aaatccttta catcttttaa aaaatatggt atattaagtc
     2701 aaagcaggca taacatatat gttatgtttt atagtaaaaa tggactattc aactatttaa
     2761 aaaatggtag aatacaaata attttcctgg cagtcaatta ttttttaaaa atttttacta
     2821 ttatattttt aaattttaaa ttttagatag agttatttgg cgataactct ttttgtctta
     2881 cttctgagtt tagcatattt tttttgtttt gtaaatccat ttctttattt ttctttacaa
     2941 cataaacttt ttttacttct ctataaatct tctcaccagt tgaaacatca atttctaaca
     3001 gttctctagc aataaaattt ttaatataga gtaaatcttt ttcatctgta agtatatcat
     3061 tcatttcatc aaaatccaac ttttttttgg tttcttttgg ttttattaaa tttcgtgaac
     3121 agaaataggc ttttttttga tttgctttct tttctaagtc tttcacaaaa tacttcgtta
     3181 tatacacccc cacagcttca tttttaacag tttctttaat cttatttatt tttataaatc
     3241 cattcgccca caattcccca agcttcttat tatctatata tgggaccgag aacagcacca
     3301 aatgataatg gattgctccc ctctgctgga gttcccaagt tgcaatatat ttaagctgtt
     3361 gatttttcaa gtaccttttc aatctcttaa taaataaagt aaattcacga tttgcccttt
     3421 ctatatcctg tatattttct ttaaaagtaa gagttaaaaa ggtactttta tcatcataat
     3481 tacaatcaat tatccttttt atttcaaatc gcttgtcctg gtaatatgct ttccttcgtt
     3541 gcatactttc agctttttca atgctactta attcttcaaa atctcttctt tttcttcctg
     3601 gtcttctttt aaagtcaaaa gcagttaaag catcaatatt gaaaatgtat tcagttatat
     3661 tttttgattt tatttcttta gtgttataaa atttcaactc aataaattcc ccctttatta
     3721 atctgcataa tcttttttat aatgtccttt acaacctaat attcttaatc tatcattttt
     3781 taagtcataa attagtctat gttcatcact tattcttctg ctataaatag ccctattttt
     3841 aaaatgtttt aatctctcag gcttaccagt accgcggccg ctccagtaat gacctcagaa
     3901 ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat
     3961 ccaagcacta gtcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4021 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4081 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4141 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     4201 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4261 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4321 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4381 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4441 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4501 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atcttta
//
