LOCUS       sequence-377316-        2844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector encoding zebrafish si:ch73-281n10.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2844)
  TITLE     Zebrafish orthologs of chromosome 21 genes
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDONR221-si:ch73-281n10.2
FEATURES             Location/Qualifiers
     source          1..2844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(969..1068)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1085..1104)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1086..1104)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1109..1125)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1109..1125)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1227..1246)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1238..2047
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1305..1324)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2194..2782
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2683..2702
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata ccaccatgcc taaaaggagc aaagctaaca atgacactga
      721 agcagctggg cccaagagac ggtctgagag acttcagggt aaaccccaaa cgccaaagtc
      781 tgagcccaag ccaaagaaag cacctgcacg aggaaagaag gccaaggatg tagaaaaggc
      841 caaaccagaa gagaagaaag aggacgcaaa gccagaagcc gaggagaaaa ctccagcgga
      901 gaacggagag accaaagctg atgaaaacaa cggagaggca ggaggtgacg acgaaaaagc
      961 tgaataagac ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     1021 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     1081 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     1141 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     1201 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
     1261 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
     1321 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc
     1381 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     1441 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     1501 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt
     1561 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     1621 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
     1681 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
     1741 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc
     1801 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     1861 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
     1921 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
     1981 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
     2041 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg
     2101 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     2161 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2221 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2281 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2341 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2401 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2461 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2521 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2581 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2641 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2701 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2761 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2821 ttgctggcct tttgctcaca tgtt
//
