LOCUS       sequence-377325-        5539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  1st generation E. coli - Fusobacterium nucleatum shuttle vector for 
            overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Ponath F, Tawk C, Zhu Y, Barquist L, Faber F, Vogel J
  TITLE     RNA landscape of the emerging cancer-associated microbe 
            Fusobacterium nucleatum.
  JOURNAL   Nat Microbiol. 2021 Aug;6(8):1007-1020. doi: 
            10.1038/s41564-021-00927-7. Epub 2021 Jul 8.
  PUBMED    34239075
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-021-00927-7"; journalName: "Nat Microbiol"; date: 
            "2021-08"; volume: "6"; issue: "8"; pages: "1007-1020"
COMMENT     Sequence Label: pEcoFus
FEATURES             Location/Qualifiers
     source          1..5539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1422..1433)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(2764..3135)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            3168..3277
                     /note="incP origin of transfer"
     primer_bind     complement(4468..4485)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4639..5227)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4719..4738)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatattctt ttttaaactt ttcaaatgct tcaaattcta catctgcaac ttctacatct
       61 tttccagtat ttccaaactt cacacaattt ttgtaaacat ttcttaattt ttccaagtat
      121 tctatgtctt ccttttcttc tttaacatac tcttgtaact gtgtcatcaa attagttttg
      181 tcttccacat tccccctttt tcttgtaatt gccttattta attcttcaaa tcttattgtt
      241 ttcattttat tgtacctcca atttttttat atatctatgt taaaaaacat attgacaatt
      301 gattaaaaca atgatattat ggtcggttga gctcattgag ctgagtgcag actttccccc
      361 ttgtaaaaag gggaaaaaac gattgataat gatacaatgg tcgtatatcg ctatgttcgc
      421 atcgtttaac ttactttatc gcatatttaa ttatatgcca gtgttagccc tgcccaacac
      481 ctagagtttt agcttacttt ctaaattata accctaataa agtacccact cagagcacct
      541 ccaagacttt taaaggtagc ctcgcacctt gctacaattc ataataccac cattacggtg
      601 ctataccgtt tcgagcagaa cggctataca tacgaattta tcttatgtat aaaatttgtt
      661 acaaatataa tagtaagttt tttttatata aaaaagcaga tcgcttttat tttttttgca
      721 acctgcttca caactcattt ttttacttta gtaaataaat cctttacatc ttttaaaaaa
      781 tatggtatat taagtcaaag caggcataac atatatgtta tgttttatag taaaaatgga
      841 ctattcaact atttaaaaaa tggtagaata caaataattt tcctggcagt caattatttt
      901 ttaaaaattt ttactattat atttttaaat tttaaatttt agatagagtt atttggcgat
      961 aactcttttt gtcttacttc tgagtttagc atattttttt tgttttgtaa atccatttct
     1021 ttatttttct ttacaacata aacttttttt acttctctat aaatcttctc accagttgaa
     1081 acatcaattt ctaacagttc tctagcaata aaatttttaa tatagagtaa atctttttca
     1141 tctgtaagta tatcattcat ttcatcaaaa tccaactttt ttttggtttc ttttggtttt
     1201 attaaatttc gtgaacagaa ataggctttt ttttgatttg ctttcttttc taagtctttc
     1261 acaaaatact tcgttatata cacccccaca gcttcatttt taacagtttc tttaatctta
     1321 tttattttta taaatccatt cgcccacaat tccccaagct tcttattatc tatatatggg
     1381 accgagaaca gcaccaaatg ataatggatt gctcccctct gctggagttc ccaagttgca
     1441 atatatttaa gctgttgatt tttcaagtac cttttcaatc tcttaataaa taaagtaaat
     1501 tcacgatttg ccctttctat atcctgtata ttttctttaa aagtaagagt taaaaaggta
     1561 cttttatcat cataattaca atcaattatc ctttttattt caaatcgctt gtcctggtaa
     1621 tatgctttcc ttcgttgcat actttcagct ttttcaatgc tacttaattc ttcaaaatct
     1681 cttctttttc ttcctggtct tcttttaaag tcaaaagcag ttaaagcatc aatattgaaa
     1741 atgtattcag ttatattttt tgattttatt tctttagtgt tataaaattt caactcaata
     1801 aattccccct ttattaatct gcataatctt ttttataatg tcctttacaa cctaatattc
     1861 ttaatctatc attttttaag tcataaatta gtctatgttc atcacttatt cttctgctat
     1921 aaatagccct atttttaaaa tgttttaatc tctcaggctt accagtaccg cggccgcctc
     1981 ccaacataaa catatattgc ttgttctaac tcatattttt gtaaaatttc aacatcatat
     2041 ccagtcaaat cgtactctct cttatataca aatggttttt ctaatttttt gttcaataaa
     2101 tctcctccta tttcttactt aatttagcta aaaatctatt tatatttcac ctcctttttg
     2161 actttaagcc tacgaatacc atatttatga ttataacatc ttctattttt cccaaatcct
     2221 tacatctccc ctaattttta gacttaaggg cgaattctgc atcaagctag cataaaaata
     2281 agaagcctgc atttgcaggc ttcttatttt tatggtacct taaaaacaaa gataagagaa
     2341 agaataaaaa gtgattttct taaaagaata gcgggttaat ataaagttga caaaaaagaa
     2401 taaaaatgat aatatatatt ggatcctata agttttaata aaactttaaa tagaaaaagg
     2461 cttctctcat gagaagtctt ttttatttaa aataaatata aaataaaata gaggctataa
     2521 atagcctcta ttttatgtga gaaatcccta aataaaaaga tgccagtgtg ctggaattcg
     2581 cccttaggct gcgctaggga cctctttagc tccttggaag ctgtcagtag taggggatcg
     2641 gtcttgcctt gctcgtcggt gatgtacttc accagctccg cgaagtcgct cttcttgatg
     2701 gagcgcatgg ggacgtgctt ggcaatcacg cgcacccccc ggccgtttta gcggctaaaa
     2761 aagtcatggc tctgccctcg ggcggaccac gcccatcatg accttgccaa gctcgtcctg
     2821 cttctcttcg atcttcgcca gcagggcgag gatcgtggca tcaccgaacc gcgccgtgcg
     2881 cgggtcgtcg gtgagccaga gtttcagcag gccgcccagg cggcccaggt cgccattgat
     2941 gcgggccagc tcgcggacgt gctcatagtc cacgacgccc gtgattttgt agccctggcc
     3001 gacggccagc aggtaggccg acaggctcat gccggccgcc gccgcctttt cctcaatcgc
     3061 tcttcgttcg tctggaaggc agtacacctt gataggtggg ctgcccttcc tggttggctt
     3121 ggtttcatca gccatccgct tgccctcatc tgttacgccg gcggtagccg gccagcctcg
     3181 cagagcagga ttcccgttga gcaccgccag gtgcgaataa gggacagtga agaaggaaca
     3241 cccgctcgcg ggtgggccta cttcacctat cctgcccggc tgacgccgtt ggatacacca
     3301 aggaaagtct acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata
     3361 taccgaaaaa atcgctataa tgaccccgaa gcaggaaggg cgaattctgc agatctgacc
     3421 ggtctctgaa aatataaaaa ccacagattg atactaaaac cttggttgtg ttgcttttcg
     3481 gggcttaaat caaggaaaaa tccttgtttt aagcctttca aaaagaaaca caaggtcttt
     3541 gtactaacct gtggttatgt ataaaattgt agattttagg gtaacaaaaa acaccgtatt
     3601 tctacgatgt ttttgcttaa atacttgttt ttagttacag acaaacctga agttaactat
     3661 ttatcaattc ctgcaattcg tttacaaaac ggcaaatgtg aaatccgtca catactgcgt
     3721 gatgaacttg aattgccaaa ggaagtataa ttttgttatc ttctttataa tatttcccca
     3781 tagtaaaaat aggaatcaaa taatcatatc ctttctgcaa attcagatta aagccatcga
     3841 aggttgacca cggtatcata gatacattaa aaatgttttc cggagcattt ggctttcctt
     3901 ccattctatg attgtttcca taccgttgcg tatcactttc ataatctgct aaaaatgatt
     3961 taaagtcaga cttacactca gtccaaaggc tggaaaatgt ttcagtatca ttgtgaaata
     4021 ttgtatagct tggtatcatc tcatcatata tccccaattc accatcttga ttgattgccg
     4081 tcctaaactc tgaatggcgg tttacaatca ttgcaatata ataaagcatt gcaggatata
     4141 gtttcattcc cttttccttt atttgtgtga tatccacttt aacggtcatg ctgtaggtac
     4201 aaggtacact tgcaaagtag tggtcaaaat actcttttct gttccaacta tttttatcaa
     4261 ttttttcaaa taccatctaa gttccctctc aaattcaagt ttatcgctct aatgaacaaa
     4321 gatattatac cacatttttg tgaatttttc aacttgccca cttcgactgc actcccgact
     4381 taataacttc ttgaacactt gccgaaaaag aaaaactgcc gggtacgtac ccggggatcg
     4441 atccccggcc gagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4501 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4561 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4621 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4681 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4741 ttgaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4801 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4861 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4921 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4981 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5041 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     5101 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5161 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5221 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5281 acgttaaggg attttggtca tgaaatgcaa gtttctaact aacatacatc atagttacta
     5341 aactatggta actataattt tattaactat attaagcact gattagtact ataactcaat
     5401 ataagcatat cccctgtatc gtaactagag aaccaaacga cggaaaaagc gatatagata
     5461 agtttagata gtgcaccaat tttttacttt tctattttag aatctagcat ttccaatgct
     5521 tactaatacc gatcgttct
//
