LOCUS       sequence-377331-        3801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector encoding zebrafish kcnj6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3801)
  TITLE     Zebrafish orthologs of chromosome 21 genes
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDONR221-kcnj6
FEATURES             Location/Qualifiers
     source          1..3801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1926..2025)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2042..2061)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2043..2061)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2066..2082)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2066..2082)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2184..2203)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2195..3004
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2262..2281)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3151..3739
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3640..3659
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctat ggccaagctg acagaatcca tggctcagaa tcttccagaa gtccagatgg
      721 agcaggatgt cgagagcacc gtcacggttc gacaacccaa gctgcccaag caggcgcggg
      781 acgacatccc caaaccgctg caggagcggg agcgcgccaa gcagaagacc cagcgctacg
      841 tccgcaaaga cggcaagtgc aacgtgcacc acggaaacgt gcgggagacc taccgctacc
      901 tgacggacat cttcaccacg ctggtggacc tcaagtggag gttcaacctg ttcatcttcg
      961 tgctggtcta caccgtcacc tggctgttct tcggcttcat gtggtggctg atcgcctaca
     1021 ttcgaggaga tctggagcac atcggagaca accagtggac tccctgcgtc aacaacctga
     1081 acggcttcgt gtccgcattc ctgttctcga tagagactga gaccaccatc ggctacggcc
     1141 accgcgtcat caccgacaag tgtccggagg gcatcgtgct gctgctggtg cagtcggtgc
     1201 tgggctccat cgtcaacgcc ttcatggtgg gctgcatgtt cgtcaagatc tcgcagccca
     1261 agaagcgcgc cgagacgctg gtcttctcca ccaacgccgt catctccatg cgggacggcc
     1321 ggctctgcct gatgttccgc gtgggagacc tcagaaactc gcacatcgtg gaggcgtcca
     1381 ttagagccaa gctgatcaag tccaaacaga ccaaggaggg cgagttcatc ccgctcaatc
     1441 agacggacat aaacgtgggc tacgacaccg gagacgaccg gctcttcctg gtgtctccgc
     1501 tcatcatctg ccacgagatc aaccagcaca gccccttctg ggagatctcc agagagcaca
     1561 tggccaagga ggagctggag atcgtggtga tcctggaggg catggtggag gccacaggca
     1621 tgacgtgtca ggcgcgcagc tcatacgtgg ccagcgagat caagtggggt taccgcttca
     1681 cgccagtgct gacgctggag gacggcttct acgaggtgga ctacaacagc ttccacgaga
     1741 tctacgagac caacacgccc agctgcagcg cgcgagagct ggccgagatg acggcgcgcg
     1801 cccgcctgcc gctcacctgg tcggtggcca gcaaactgag ccagctggag ccgcaggagc
     1861 cgccggagga gctgacggag aggaacggag acgtcgccaa catggagagc gagtccaaag
     1921 tgtagaccca gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
     1981 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat
     2041 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     2101 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
     2161 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
     2221 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     2281 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
     2341 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     2401 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     2461 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
     2521 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     2581 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     2641 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     2701 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     2761 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     2821 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     2881 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     2941 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     3001 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga
     3061 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     3121 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3181 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3241 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3301 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3361 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3421 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3481 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3541 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3601 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3661 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3721 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3781 ctggcctttt gctcacatgt t
//
