LOCUS       sequence-377334-       11983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana
            apoplast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11983)
  AUTHORS   Tran Q, Osabe K, Entani T, Wazawa T, Hattori M, Nagai T
  TITLE     Application of Green enhanced Nano-lantern as a bioluminescent 
            ratiometric indicator for measurement of Arabidopsis thaliana root 
            apoplastic fluid pH.
  JOURNAL   Plant Cell Environ. 2022 Jul 21. doi: 10.1111/pce.14404.
  PUBMED    35864560
REFERENCE   2  (bases 1 to 11983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Environ. 2022 Jul 21. doi: 10.1111/pce.14404."
COMMENT     Sequence Label: 35S::Cel1SP-GeNL/pCAMBIA1301
FEATURES             Location/Qualifiers
     source          1..11983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    4..28
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     216..238
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     230..247
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     231..247
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        765..1110
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     5'UTR           1109..1166
                     /label=AtADH 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the 
                     Arabidopsis thaliana alcohol dehydrogenase gene (Sugio et 
                     al., 2008"
     terminator      2440..2688
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3341..3357)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3341..3357)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3354..3376)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3365..3381
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3389..3395,3396..3413,3414..3419))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3434..3455
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3646..4322
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4389..5414
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5455..5629
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5707..5731
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6156..6950
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7037..7625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7526..7545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7779..7796
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7811..7951
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7877..7896)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8037..8059
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8295..8489
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8555..9628)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10057..10686)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac
       61 gacaatctga tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg
      121 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
      181 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
      241 ggccagtgcc aagcttgcat gcctgcaggt cgactctaga ttagcctttt caatttcaga
      301 aagaatgcta acccacagat ggttagagag gcttacgcag caggtctcat caagacgatc
      361 tacccgagca ataatctcca ggaaatcaaa taccttccca agaaggttaa agatgcagtc
      421 aaaagattca ggactaactg catcaagaac acagagaaag atatatttct caagatcaga
      481 agtactattc cagtatggac gattcaaggc ttgcttcaca aaccaaggca agtaatagag
      541 attggagtct ctaaaaaggt agttcccact gaatcaaagg ccatggagtc aaagattcaa
      601 atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca gagtctctta
      661 cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac acttgtctac
      721 tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa
      781 agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg
      841 aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc
      901 atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      961 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     1021 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     1081 taaggaagtt catttcattt ggagagaata catcacaatc acacaaaact aacaaaagat
     1141 caaaagcaag ttcttcactg ttgataatgc atatggcgcg aaaatcccta attttcccgg
     1201 tgattttgct cgccgttctt ctcttctctc cgccgattta ctccgccatg gtgtccaagg
     1261 gcgaagagga caacatggcc agcctgcctg ccacccacga gctgcacatc ttcggcagca
     1321 tcaacggcgt ggacttcgac atggtgggac agggcaccgg caaccccaac gacggctacg
     1381 aggaactgaa cctgaagtcc acaaagggcg acctgcagtt cagcccctgg attctggtgc
     1441 cccacatcgg ctacggcttc caccagtacc tgccctaccc cgacggcatg agccctttcc
     1501 aggccgctat ggtggatggc agcggctacc aggtgcaccg gaccatgcag tttgaggacg
     1561 gcgccagcct gaccgtgaac taccggtaca catacgaggg cagccacatc aagggcgagg
     1621 cccaagtgaa gggcacaggc tttccagccg acggccccgt gatgaccaat agcctgacag
     1681 ccgccgactg gtgcagaagc aagaaaacct accccaatga caagaccatc atcagcacct
     1741 tcaagtggtc ctacaccacc ggcaatggca agcggtacag aagcaccgcc cggaccacct
     1801 acaccttcgc caaacctatg gccgccaact acctgaagaa ccagcctatg tacgtgttcc
     1861 gcaagaccga gctgaagcac tccaagacag aactgaactt caaagagtgg cagaaagcct
     1921 tcaccgggtt tgaagatttc gttggggact ggcgacagac agccggctac aacctggacc
     1981 aagtccttga acagggaggt gtgtccagtt tgtttcagaa tctcggggtg tccgtaactc
     2041 cgatccaaag gattgtcctg agcggtgaaa atgggctgaa gatcgacatc catgtcatca
     2101 tcccgtatga aggtctgagc ggcgaccaaa tgggccagat cgaaaaaatt tttaaggtgg
     2161 tgtaccctgt ggatgatcat cactttaagg tgatcctgca ctatggcaca ctggtaatcg
     2221 acggggttac gccgaacatg atcgactatt tcggacggcc gtatgaaggc atcgccgtgt
     2281 tcgacggcaa aaagatcact gtaacaggga ccctgtggaa cggcaacaaa attatcgacg
     2341 agcgcctgat caaccccgac ggctccctgc tgttccgagt aaccatcaac ggagtgaccg
     2401 gctggcggct gtgcgaacgc attctggcgt aagagctcat atgaagatga agatgaaata
     2461 tttggtgtgt caaataaaaa gctagcttgt gtgcttaagt ttgtgttttt ttcttggctt
     2521 gttgtgttat gaatttgtgg ctttttctaa tattaaatga atgtaagatc tcattataat
     2581 gaataaacaa atgtttctat aatccattgt gaatgttttg ttggatctct tcgcatataa
     2641 ctactgtatg tgctatggta tggactatgg aatatgatta aagataagac tagatgggct
     2701 catagagtaa aacgaggcga gggacctata aacctccctt catcatgcta tttcatgatc
     2761 tattttataa aataaagatg tagaaaaaag taagcgtaat aaccgcaaaa caaatgattt
     2821 aaaacatggc acataatgag gagattaagt tcggtttacg tttattttag tactaattgt
     2881 aacgtgagac tacgtatcgg gaatcgccta attaaagcat taatgcgaac ctgattagat
     2941 tcaccgaccc tcctatcgtg tcgacctttc tgtttcttag aattttttgg tagtctatgt
     3001 actaataatg tcagcttcgt atttatttca taagcaattt gcatttgcaa tttgtttttt
     3061 acttttattt ttattgtatt gtggaatgtg gactcgtacc aacatgaagt tatataccac
     3121 caaaaaaatt acagttagtc aaaagattca cgagtgagag ctacttatga ttgtctttta
     3181 cgtatatgtc taattgtcta tttgctcaat aatctttgta ctttcttttg tcgttgataa
     3241 aatcacaaag ttccaaaagt aatcgaatga tttgctttta agaaaagaag agctgaataa
     3301 ttcaacatat atctgtacac tagtttgaat tcgtaatcat gtcatagctg tttcctgtgt
     3361 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     3421 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     3481 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     3541 gcggtttgcg tattggctag agcagcttgc caacatggtg gagcacgaca ctctcgtcta
     3601 ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca
     3661 aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa
     3721 aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc
     3781 tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
     3841 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgaaca
     3901 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
     3961 aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
     4021 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat
     4081 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca
     4141 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     4201 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
     4261 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacacgct
     4321 gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tccggggggc
     4381 aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     4441 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     4501 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     4561 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     4621 ttgacattgg ggagtttagc gagagcctga cctattgcat ctcccgccgt tcacagggtg
     4681 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctacaaccg gtcgcggagg
     4741 ctatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     4801 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     4861 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     4921 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     4981 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     5041 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     5101 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     5161 gatcgccacg actccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     5221 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     5281 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     5341 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     5401 gggcaaagaa atagagtaga tgccgaccgg gatctgtcga tcgacaagct cgagtttctc
     5461 cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc
     5521 atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca
     5581 ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa
     5641 ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt
     5701 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca
     5761 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg
     5821 gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc
     5881 aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa
     5941 cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact
     6001 atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt
     6061 tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc
     6121 tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg
     6181 aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg
     6241 ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc
     6301 ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag
     6361 gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc
     6421 tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg
     6481 aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg
     6541 attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata
     6601 acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc
     6661 gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg
     6721 gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc
     6781 ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca
     6841 gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc
     6901 ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat
     6961 gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7021 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7081 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7141 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     7201 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7261 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7321 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7381 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7441 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7501 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7561 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7621 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7681 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7741 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7801 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     7861 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     7921 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     7981 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     8041 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt
     8101 gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc
     8161 ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg
     8221 cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc
     8281 gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag
     8341 agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc
     8401 ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct
     8461 ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg
     8521 ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct
     8581 cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca
     8641 aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct
     8701 tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg
     8761 ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca
     8821 aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt
     8881 acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga
     8941 tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg
     9001 ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca
     9061 ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt
     9121 gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt
     9181 cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg
     9241 ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag
     9301 ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc
     9361 gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg
     9421 gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat
     9481 cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg
     9541 cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta
     9601 ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc
     9661 attgcagggc cggcaggcaa cccagccgct tacgcctggc caaccgcccg ttcctccaca
     9721 catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt
     9781 agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga
     9841 tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct
     9901 tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca
     9961 ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt
    10021 ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc
    10081 agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt
    10141 tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat
    10201 gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat
    10261 cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt
    10321 aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat
    10381 cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc
    10441 gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg
    10501 gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact
    10561 gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc
    10621 tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac
    10681 cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc
    10741 gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt
    10801 tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg
    10861 atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg
    10921 atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc
    10981 ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg
    11041 tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc
    11101 tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt
    11161 tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga
    11221 gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg
    11281 tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca
    11341 tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc
    11401 cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt
    11461 ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct
    11521 ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg
    11581 cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc
    11641 ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag
    11701 tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg
    11761 cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg
    11821 aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc
    11881 gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg
    11941 cccttttaaa tatccgatta ttctaataaa cgctcttttc tct
//
