LOCUS       sequence-377338-        9646 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Addgene #103871 with 3x FLAG on N-terminal of dCas13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Farrell K, Musaus M, Auerbach A, Navabpour S, Ray WK, Helm RF, 
            Jarome TJ
  TITLE     Proteasome-independent K63 polyubiquitination selectively regulates 
            ATP levels and proteasome activity during fear memory formation in 
            the female amygdala.
  JOURNAL   Mol Psychiatry. 2023 May 17. doi: 10.1038/s41380-023-02112-0.
  PUBMED    37198264
REFERENCE   2  (bases 1 to 9646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Psychiatry. 2023 May 17. doi: 10.1038/s41380-023-02112-0."
COMMENT     Sequence Label: FLAG-dCas13b-ADAR2DD
FEATURES             Location/Qualifiers
     source          1..9646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             916..942
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(2151..2168)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2157..2381
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2427..2855
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2514..2533)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2724..2745
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2864..2884)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2869..3198
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3049..3184
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3111..3130
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3265..4059
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3319..3338)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3929..3948
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4233..4354
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4270..4289)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4324..4343
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4403..4419)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4403..4419)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4416..4438)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4427..4443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4451..4457,4458..4475,4476..4481))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4496..4517
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4634..4651)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4805..5393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4885..4904)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5564..6355,6356..6424))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6187..6206
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6425..6529)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6604..6623)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6795..7174
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7175..7378
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7329..7349
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7423..7442
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7423..7441
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      7469..7478
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7478..7543
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7550..7576
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
ORIGIN
        1 aatgccgtcg agttctacga gcgctacctg atcgagcgga agttctacct gaccggcctg
       61 tccaacgaga tcaagaaagg caacagagtg gatgtgccct tcatccggcg ggaccagaac
      121 aagtggaaaa cacccgccat gaagaccctg ggcagaatct acagcgagga tctgcccgtg
      181 gaactgccca gacagatgtt cgacaatgag atcaagtccc acctgaagtc cctgccacag
      241 atggaaggca tcgacttcaa caatgccaac gtgacctatc tgatcgccga gtacatgaag
      301 agagtgctgg acgacgactt ccagaccttc taccagtgga accgcaacta ccggtacatg
      361 gacatgctta agggcgagta cgacagaaag ggctccctgc agcactgctt caccagcgtg
      421 gaagagagag aaggcctctg gaaagagcgg gcctccagaa cagagcggta cagaaagcag
      481 gccagcaaca agatccgcag caaccggcag atgagaaacg ccagcagcga agagatcgag
      541 acaatcctgg ataagcggct gagcaacagc cggaacgagt accagaaaag cgagaaagtg
      601 atccggcgct acagagtgca ggatgccctg ctgtttctgc tggccaaaaa gaccctgacc
      661 gaactggccg atttcgacgg cgagaggttc aaactgaaag aaatcatgcc cgacgccgag
      721 aagggaatcc tgagcgagat catgcccatg agcttcacct tcgagaaagg cggcaagaag
      781 tacaccatca ccagcgaggg catgaagctg aagaactacg gcgacttctt tgtgctggct
      841 agcgacaaga ggatcggcaa cctgctggaa ctcgtgggca gcgacatcgt gtccaaagag
      901 gatggatccc ttcaactgcc tccacttgaa agactgacac tgggatccca gctgcattta
      961 ccgcaggttt tagctgacgc tgtctcacgc ctggtcctgg gtaagtttgg tgacctgacc
     1021 gacaacttct cctcccctca cgctcgcaga aaagtgctgg ctggagtcgt catgacaaca
     1081 ggcacagatg ttaaagatgc caaggtgata agtgtttcta caggaggcaa atgtattaat
     1141 ggtgaataca tgagtgatcg tggccttgca ttaaatgact gccatgcaga aataatatct
     1201 cggagatcct tgctcagatt tctttataca caacttgagc tttacttaaa taacaaagat
     1261 gatcaaaaaa gatccatctt tcagaaatca gagcgagggg ggtttaggct gaaggagaat
     1321 gtccagtttc atctgtacat cagcacctct ccctgtggag atgccagaat cttctcacca
     1381 catgagccaa tcctggaaga accagcagat agacacccaa atcgtaaagc aagaggacag
     1441 ctacggacca aaatagagtc tggtcagggg acgattccag tgcgctccaa tgcgagcatc
     1501 caaacgtggg acggggtgct gcaaggggag cggctgctca ccatgtcctg cagtgacaag
     1561 attgcacgct ggaacgtggt gggcatccag ggatcactgc tcagcatttt cgtggagccc
     1621 atttacttct cgagcatcat cctgggcagc ctttaccacg gggaccacct ttccagggcc
     1681 atgtaccagc ggatctccaa catagaggac ctgccacctc tctacaccct caacaagcct
     1741 ttgctcagtg gcatcagcaa tgcagaagca cggcagccag ggaaggcccc caacttcagt
     1801 gtcaactgga cggtaggcga ctccgctatt gaggtcatca acgccacgac tgggaaggat
     1861 gagctgggcc gcgcgtcccg cctgtgtaag cacgcgttgt actgtcgctg gatgcgtgtg
     1921 cacggcaagg ttccctccca cttactacgc tccaagatta ccaagcccaa cgtgtaccat
     1981 gagtccaagc tggcggcaaa ggagtaccag gccgccaagg cgcgtctgtt cacagccttc
     2041 atcaaggcgg ggctgggggc ctgggtggag aagcccaccg agcaggacca gttctcactc
     2101 acgtaagcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     2161 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     2221 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     2281 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     2341 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     2401 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     2461 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     2521 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2581 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     2641 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     2701 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     2761 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     2821 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     2881 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     2941 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3001 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     3061 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3121 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     3181 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     3241 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     3301 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     3361 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     3421 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     3481 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     3541 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     3601 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     3661 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     3721 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     3781 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     3841 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     3901 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     3961 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     4021 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     4081 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     4141 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     4201 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     4261 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4321 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     4381 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     4441 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     4501 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     4561 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     4621 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4681 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4741 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4801 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4861 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4921 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4981 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5041 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5101 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5161 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5221 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5281 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5341 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5401 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5461 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     5521 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5581 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5641 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5701 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     5761 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     5821 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     5881 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     5941 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6001 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6061 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6121 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6181 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6241 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6301 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6361 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6421 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     6481 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     6541 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     6601 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     6661 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     6721 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     6781 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     6841 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     6901 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     6961 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     7021 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     7081 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     7141 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     7201 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     7261 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     7321 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
     7381 ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag
     7441 ggagacccaa gctggctagc gtttaaacgc caccatggac tataaggacc acgacggaga
     7501 ctacaaggat catgatattg attacaaaga cgatgacgat aagatggccc tgcctccact
     7561 tgaaagactg acactgggta tcaaacttaa gcttgccacc atgaacatcc ccgctctggt
     7621 ggaaaaccag aagaagtact ttggcaccta cagcgtgatg gccatgctga acgctcagac
     7681 cgtgctggac cacatccaga aggtggccga tattgagggc gagcagaacg agaacaacga
     7741 gaatctgtgg tttcaccccg tgatgagcca cctgtacaac gccaagaacg gctacgacaa
     7801 gcagcccgag aaaaccatgt tcatcatcga gcggctgcag agctacttcc cattcctgaa
     7861 gatcatggcc gagaaccaga gagagtacag caacggcaag tacaagcaga accgcgtgga
     7921 agtgaacagc aacgacatct tcgaggtgct gaagcgcgcc ttcggcgtgc tgaagatgta
     7981 cagggacctg accaacgcat acaagaccta cgaggaaaag ctgaacgacg gctgcgagtt
     8041 cctgaccagc acagagcaac ctctgagcgg catgatcaac aactactaca cagtggccct
     8101 gcggaacatg aacgagagat acggctacaa gacagaggac ctggccttca tccaggacaa
     8161 gcggttcaag ttcgtgaagg acgcctacgg caagaaaaag tcccaagtga ataccggatt
     8221 cttcctgagc ctgcaggact acaacggcga cacacagaag aagctgcacc tgagcggagt
     8281 gggaatcgcc ctgctgatct gcctgttcct ggacaagcag tacatcaaca tctttctgag
     8341 caggctgccc atcttctcca gctacaatgc ccagagcgag gaacggcgga tcatcatcag
     8401 atccttcggc atcaacagca tcaagctgcc caaggaccgg atccacagcg agaagtccaa
     8461 caagagcgtg gccatggata tgctcaacga agtgaagcgg tgccccgacg agctgttcac
     8521 aacactgtct gccgagaagc agtcccggtt cagaatcatc agcgacgacc acaatgaagt
     8581 gctgatgaag cggagcagcg acagattcgt gcctctgctg ctgcagtata tcgattacgg
     8641 caagctgttc gaccacatca ggttccacgt gaacatgggc aagctgagat acctgctgaa
     8701 ggccgacaag acctgcatcg acggccagac cagagtcaga gtgatcgagc agcccctgaa
     8761 cggcttcggc agactggaag aggccgagac aatgcggaag caagagaacg gcaccttcgg
     8821 caacagcggc atccggatca gagacttcga gaacatgaag cgggacgacg ccaatcctgc
     8881 caactatccc tacatcgtgg acacctacac acactacatc ctggaaaaca acaaggtcga
     8941 gatgtttatc aacgacaaag aggacagcgc cccactgctg cccgtgatcg aggatgatag
     9001 atacgtggtc aagacaatcc ccagctgccg gatgagcacc ctggaaattc cagccatggc
     9061 cttccacatg tttctgttcg gcagcaagaa aaccgagaag ctgatcgtgg acgtgcacaa
     9121 ccggtacaag agactgttcc aggccatgca gaaagaagaa gtgaccgccg agaatatcgc
     9181 cagcttcgga atcgccgaga gcgacctgcc tcagaagatc ctggatctga tcagcggcaa
     9241 tgcccacggc aaggatgtgg acgccttcat cagactgacc gtggacgaca tgctgaccga
     9301 caccgagcgg agaatcaaga gattcaagga cgaccggaag tccattcgga gcgccgacaa
     9361 caagatggga aagagaggct tcaagcagat ctccacaggc aagctggccg acttcctggc
     9421 caaggacatc gtgctgtttc agcccagcgt gaacgatggc gagaacaaga tcaccggcct
     9481 gaactaccgg atcatgcaga gcgccattgc cgtgtacgat agcggcgacg attacgaggc
     9541 caagcagcag ttcaagctga tgttcgagaa ggcccggctg atcggcaagg gcacaacaga
     9601 gcctcatcca tttctgtaca aggtgttcgc ccgcagcatc cccgcc
//
