LOCUS       sequence-37734-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Bonger KM, Chen LC, Liu CW, Wandless TJ
  TITLE     Small-molecule displacement of a cryptic degron causes conditional 
            protein degradation.
  JOURNAL   Nat Chem Biol. 2011 Jul 3. doi: 10.1038/nchembio.598.
  PUBMED    21725303
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2011 Jul 3. doi: 10.1038/nchembio.598."
COMMENT     Sequence Label: pBMN YFP-LID
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     307..323
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             387..413
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(459..480)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(720..739)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gagatcttat atggggcacc cccgcccctt gtaaacttcc ctgaccctga catgacaaga
       61 gttactaaca gcccctctct ccaagctcac ttacaggctc tctacttagt ccagcacgaa
      121 gtctggagac ctctggcggc agcctaccaa gaacaactgg accgaccggt ggtacctcac
      181 ccttaccgag tcggcgacac agtgtgggtc cgccgacacc agactaagaa cctagaacct
      241 cgctggaaag gaccttacac agtcctgctg accaccccca ccgccctcaa agtagacggc
      301 atcgcagctt ggatacacgc cgcccacgtg aaggctgccg accccggggg tggaccatcc
      361 tctagactgc cggatccgcc accatgtatc cgtacgacgt accggattat gcgatggtga
      421 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      481 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      541 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      601 ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
      661 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      721 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      781 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
      841 agtacaacta caacagccac aacgtctata tcat
//
