LOCUS       sequence-377344-       11886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ATAD2 overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11886)
  AUTHORS   Baggiolini A, Callahan SJ, Montal E, Weiss JM, Trieu T, Tagore MM, 
            Tischfield SE, Walsh RM, Suresh S, Fan Y, Campbell NR, Perlee SC, 
            Saurat N, Hunter MV, Simon-Vermot T, Huang TH, Ma Y, Hollmann T, 
            Tickoo SK, Taylor BS, Khurana E, Koche RP, Studer L, White RM
  TITLE     Developmental chromatin programs determine oncogenic competence in 
            melanoma.
  JOURNAL   Science. 2021 Sep 3;373(6559):eabc1048. doi: 
            10.1126/science.abc1048. Epub 2021 Sep 3.
  PUBMED    34516843
REFERENCE   2  (bases 1 to 11886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2021-09-3"; volume: "373(6559):eabc1048. doi"; pages: " 
            10.1126/science.abc1048. Epub 2021 Sep 3"
COMMENT     Sequence Label: lenti-ATAD2-plx303
FEATURES             Location/Qualifiers
     source          1..11886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    603..836
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1021..1065
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1240..1740
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1659..1677
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1752..2147
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2207..2324
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2441..2744
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2745..2948
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2899..2919
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2971..2995
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3824..3844)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    complement(7202..7226)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7269..7857
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7322..7342)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7740..7751)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7929..8162
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8240..8361
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8277..8296)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8331..8350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8401..8536
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8556..8575)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8557..8575)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8585..8602)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8585..8601)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8594..8616)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8743..9198
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8830..8849)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9040..9061
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9224..9328
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9329..9397,9398..10189)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9547..9566)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10360..10948
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10849..10868
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11102..11119
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11236..11257
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11272..11277,11278..11295,11296..11302)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11310..11326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11315..11337
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11334..11350
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11334..11350
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11369..11389
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11371..11389
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        11417..11643
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             11644..11824
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    join(11871..11886,1..110)
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
       61 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      121 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
      181 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
      241 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
      301 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
      361 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
      421 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
      481 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
      541 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
      601 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
      661 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
      721 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
      781 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
      841 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
      901 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
      961 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1021 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1081 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1141 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     1201 ttatcgtttc agacccacct cccaaccccg aggggaccct tgcgcctttt ccaaggcagc
     1261 cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc
     1321 gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct
     1381 acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg
     1441 cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca
     1501 gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc
     1561 ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg
     1621 tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct
     1681 ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag
     1741 ggggtaccac catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa
     1801 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct
     1861 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg
     1921 cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg
     1981 tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc
     2041 ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg
     2101 cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggcctg cagctgcagt
     2161 agtaagaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     2221 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2281 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2341 acagcagaga tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa
     2401 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     2461 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2521 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2581 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2641 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2701 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2761 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2821 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2881 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2941 gcagagctct ctggctaact gtcgggatca acaagtttgt acaaaaaagc aggctccgcg
     3001 gccgccccct tcaccatggt ggttctccgc agcagcttgg agctgcacaa ccactccgcg
     3061 gcctcggcca cgggctcctt ggacctgtcc agtgacttcc tcagtctgga gcacatcggc
     3121 cggaggcggc tccgctcggc cggcgcggcg cagaagaaac ccgcggcgac cacagccaaa
     3181 gcgggcgatg ggtcatcagt taaggaagtt gaaacctacc accggacacg tgctttaaga
     3241 tctttgagaa aagatgcaca gaattcttca gattctagtt ttgagaagaa tgtggaaata
     3301 acggagcaac ttgctaatgg caggcatttt acaaggcagt tggccagaca gcaggctgat
     3361 aaaaaaaaag aagagcacag agaagacaaa gtgattccag ttactcggtc attgagggct
     3421 agaaacatcg ttcaaagtac agaacactta catgaagata atggtgatgt tgaagtgcgt
     3481 cgaagttgta ggattagaag tcgttatagt ggtgtaaacc agtccatgct gtttgacaaa
     3541 cttataacta acactgctga agctgtactt caaaaaatgg atgacatgaa gaagatgcgt
     3601 agacagcgaa tgagagaact tgaagacttg ggagtgttta atgaaacaga agaaagcaat
     3661 cttaatatgt acacaagagg aaaacagaaa gatattcaaa gaactgatga agaaacaact
     3721 gataatcaag aaggcagtgt ggagtcatct gaagagggtg aagaccaaga acatgaagat
     3781 gatggtgaag atgaagatga tgaagatgat gatgatgatg acgatgatga tgatgatgat
     3841 gatgaagatg atgaagatga agaagatgga gaagaagaga atcagaagcg atattatctt
     3901 agacagagaa aagctactgt ttactatcag gctccattgg aaaaacctcg tcaccagaga
     3961 aagcccaaca tattttatag tggcccagct tctcctgcaa gaccaagata ccgattatct
     4021 tccgcaggac caagaagtcc ttactgtaaa cgaatgaaca ggcgaaggca tgcaatccac
     4081 agtagtgact cgacttcatc ttcctcctct gaagatgaac agcactttga gaggcggagg
     4141 aaaaggagtc gtaatagggc tatcaatagg tgcctcccac taaattttcg gaaagatgaa
     4201 ttaaaaggca tttataaaga tcgaatgaaa attggagcaa gccttgccga tgttgatcca
     4261 atgcaactag attcttcagt acgatttgat agtgttggtg gcctgtctaa tcatatagca
     4321 gctctaaaag agatggtggt gtttccatta ctttatccag aagtctttga aaaatttaaa
     4381 attcaacccc caagaggttg tttgttttat gggccacctg gaactggaaa gactctggtt
     4441 gccagagcac ttgccaatga gtgcagtcaa ggggataaaa gagtagcatt tttcatgagg
     4501 aaaggtgctg attgtctaag taaatgggta ggagaatctg aaagacagct acgattgctg
     4561 tttgatcagg cctatcagat gcgcccatca attatttttt ttgacgaaat tgatggtctg
     4621 gctccagtac ggtcaagcag gcaagatcag attcacagtt ctattgtttc caccctgcta
     4681 gctcttatgg atggattgga cagcagaggg gaaattgtgg tcattggtgc tacgaacagg
     4741 ctagattcta tagatcctgc tttacgaagg cctggtcgct ttgatagaga attcctcttt
     4801 agcctgcctg ataaagaggc tcgaaaagag attctaaaga ttcacaccag ggattggaat
     4861 cccaaaccac tggacacatt tttagaagag ctagcagaaa actgtgttgg atactgtgga
     4921 gcagatatta aatcaatatg tgctgaagct gctttatgtg ctttacgacg acgctaccca
     4981 cagatctata ccactagtga gaaactgcag ttggatctct cttcaattaa tatctcagct
     5041 aaggatttcg aggtagctat gcaaaagatg ataccagcct cccaaagagc tgtgacatca
     5101 cctgggcagg cactgtccac cgttgtgaaa ccactcctgc aaaacactgt tgacaagatt
     5161 ttagaagccc tgcagagagt atttccacat gcagaattca gaacaaataa aacattagac
     5221 tcagatattt cttgtcctct gctagaaagt gacttggctt acagtgatga tgatgttcca
     5281 tcagtttatg aaaatggact ttctcagaaa tcttctcata aggcaaaaga caattttaat
     5341 tttcttcatt tgaatagaaa tgcttgttac caacctatgt cttttcgacc aagaatattg
     5401 atagtaggag aaccaggatt tgggcaaggt tctcacttgg caccagctgt cattcatgct
     5461 ttggaaaagt ttactgtata tacattagac attcctgttc tttttggagt tagtactaca
     5521 tcccctgaag aaacatgtgc ccaggtgatt cgtgaagcta agagaacagc accaagtata
     5581 gtgtatgttc ctcatatcca cgtgtggtgg gaaatagttg gaccgacact taaagccaca
     5641 tttaccacat tattacagaa tattccttca tttgctccag ttttactact tgcaacttct
     5701 gacaaacccc attccgcttt gccagaagag gtgcaagaat tgtttatccg tgattatgga
     5761 gagattttta atgtccagtt accggataaa gaagaacgga caaaattttt tgaagattta
     5821 attctaaaac aagctgctaa gcctcctata tcaaaaaaga aagcagtttt gcaggctttg
     5881 gaggtactcc cagtagcacc accacctgag ccaagatcac tgacagcaga agaagtgaaa
     5941 cgactagaag aacaagaaga agatacattt agagaactga ggattttctt aagaaatgtt
     6001 acacataggc ttgctattga caagcgattc cgagtgttta ctaagcctgt tgaccctgat
     6061 gaggttcctg attatgtcac tgtaataaag caaccaatgg acctttcatc tgtaatcagt
     6121 aaaattgatc tacacaagta tctgactgtg aaagactatt tgagagatat tgatctaatc
     6181 tgtagtaatg ccttagaata caatccagat agagatcctg gagatcgtct tattaggcat
     6241 agagcctgtg ctttaagaga tactgcctat gccataatta aagaagaact tgatgaagac
     6301 tttgagcagc tctgtgaaga aattcaggaa tctagaaaga aaagaggttg tagctcctcc
     6361 aaatatgccc cgtcttacta ccatgtgatg ccaaagcaaa attccactct tgttggtgat
     6421 aaaagatcag acccagagca gaatgaaaag ctaaagacac cgagtactcc tgtggcttgc
     6481 agcactcctg ctcagttgaa gaggaaaatt cgcaaaaagt caaactggta cttaggcacc
     6541 ataaaaaagc gaaggaagat ttcacaggca aaggatgata gccagaatgc catagatcac
     6601 aaaattgaga gtgatacaga ggaaactcaa gacacaagtg tagatcataa tgagaccgga
     6661 aacacaggag agtcttcggt ggaagaaaat gaaaaacagc aaaatgcctc tgaaagcaaa
     6721 ctggaattga gaaataattc aaatacttgt aatatagaga atgagcttga agactctagg
     6781 aagactacag catgtacaga attgagagac aagattgctt gtaatggaga tgcttctagc
     6841 tctcagataa tacatatttc tgatgaaaat gaaggaaaag aaatgtgtgt tctgcgaatg
     6901 actcgagcta gacgttccca ggtagaacag cagcagctca tcactgttga aaaggctttg
     6961 gcaattcttt ctcagcctac accctcactt gttgtggatc atgagcgatt aaaaaatctt
     7021 ttgaagactg ttgttaaaaa aagtcaaaac tacaacatat ttcagttgga aaatttgtat
     7081 gcagtaatca gccaatgtat ttatcggcat cgcaaggacc atgataaaac atcacttatt
     7141 cagaaaatgg agcaagaggt agaaaacttc agttgttcca gataaaaggg tgggcgcgcc
     7201 gacccagctt tcttgtacaa agtggtttag taatgagcta gcgctaaccg gtggcgcgtt
     7261 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     7321 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     7381 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     7441 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     7501 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     7561 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     7621 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     7681 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     7741 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     7801 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     7861 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7921 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     7981 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     8041 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     8101 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8161 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     8221 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     8281 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8341 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     8401 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8461 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8521 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     8581 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     8641 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     8701 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     8761 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     8821 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     8881 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     8941 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     9001 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     9061 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     9121 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     9181 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     9241 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     9301 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     9361 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     9421 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     9481 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     9541 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     9601 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     9661 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     9721 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     9781 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     9841 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     9901 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     9961 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    10021 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    10081 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    10141 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    10201 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    10261 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    10321 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    10381 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    10441 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    10501 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    10561 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    10621 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    10681 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    10741 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    10801 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    10861 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    10921 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    10981 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    11041 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    11101 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
    11161 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    11221 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    11281 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    11341 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
    11401 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
    11461 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
    11521 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
    11581 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
    11641 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
    11701 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
    11761 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
    11821 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
    11881 gcagga
//
