LOCUS       sequence-377345-        3846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector encoding zebrafish erg.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3846)
  TITLE     Zebrafish orthologs of chromosome 21 genes
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDONR221-erg
FEATURES             Location/Qualifiers
     source          1..3846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1971..2070)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2087..2106)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2088..2106)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2111..2127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2111..2127)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2229..2248)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2240..3049
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2307..2326)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3196..3784
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3685..3704
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata ccaccatgac ggcgtctgca gccgctgagc tccctcacag
      721 taaactcctg ctgtctgctg tctctgtcaa gctggaggcc agcgctggag cacaccggag
      781 ccactgcagg agtcctcctg atgactgtcc gggggtcaaa ggaaagatct ctccggctgg
      841 gaatgctcca gtgatgtaca gcaactacat ggaggagaaa cacacaccgc cgagcagtga
      901 gcgcagagtc atcgtacctg cagacccggg gcagtggtcg gcgggtcacg tgcggcagtg
      961 gctggactgg gcggtgcagg agtacgggct gccgccgctg gacgtgtctc tgtttcaggg
     1021 tgtggatggg aagcagctct gcaggatgag cagagaggag ctccagcgtc tgaccagcag
     1081 ctactctgca gacgtgctgc tctctcacct gcactacctc agagagactc cacttcctca
     1141 cttgacttca gatgatgttg acaaagcctt gcaaaactcc ccgcggttaa tgcatgctcg
     1201 caacacagga ggagccaact tcatctttcc caacacacca gtctaccctc cagacgccag
     1261 cagaggagcc agcagagcag atgtgggtta tgacgctgtc aggcgctctg gatggactcc
     1321 tgctcttcct gcagctaaag tttctcagag ctctgccgcc atcatgagca aaactgaaga
     1381 gcagagagcg cagttagatc cgtatcagat tttagggccg accagcagca gactggccaa
     1441 cccaggctct ggtcagatcc agctgtggca gttcctgctg gagctcctgt ccgacagctg
     1501 caactcgtcc tgcatcactt gggagggaac caacggcgag ttcaagatga cggacccgga
     1561 cgaggtggcg cgacgctggg gcgagcggaa gagcaagccc aacatgaact acgacaagct
     1621 gagtcgagcc ctgcgctact actatgacaa gaacatcatg accaaagtgc acggcaagcg
     1681 ctacgcctac aagttcgact tccatggcat tgcgcaagcc ttgcagccgc acccgcccga
     1741 ctcctccatc tacaagtacc ctgcggtggc ggcggatctg ccctacgtca gctcctacca
     1801 cccgcagaag atgagcttca tggctcctca tcctcaggcc atgagcgtct cctcctccgg
     1861 cttcttcagc gggccggctc cgtactggaa cacaccagcg gcagggatct acggcgggcc
     1921 acggcacccc cctgctcaca tgccctcaca tctgggctca tactactagg acccagcttt
     1981 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     2041 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     2101 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     2161 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     2221 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     2281 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     2341 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     2401 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     2461 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     2521 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     2581 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     2641 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     2701 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     2761 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     2821 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     2881 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     2941 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     3001 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     3061 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
     3121 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     3181 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3241 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3301 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3361 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3421 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3481 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3541 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3601 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3661 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3721 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3781 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3841 catgtt
//
