LOCUS       sequence-37739-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..220
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      229..238
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             244..270
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             244..270
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             280..300
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
       61 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
      121 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
      181 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gatctgccgc caccatgggc
      241 gtatacccct acgacgtgcc cgactacgcc cccgggcccc ctaagaaaaa gaggaaggtg
      301 aagaacatca agaagaacca ggtcatgaac ctgggcccca acagcaagct gctgaaggag
      361 tacaagagcc agctgatcga gctgaacatc gagcagttcg aggccggcat cggcctgatc
      421 ctgggcgacg cctacatcag gagcagggac gagggcaaga cctactgcat gcagttcgag
      481 tggaagaaca aggcctacat ggaccacgtg tgcctgctgt acgaccagtg ggtgctgagc
      541 cccccccaca agaaggagag ggtgaaccac ctgggcaacc tggtcatcac ctggggcgcc
      601 cagaccttca agcaccaggc cttcaacaag ctggccaacc tgttcatcgt gaacaacaag
      661 aagaccatcc ccaacaacct ggtggagaac tacctgaccc ccatgagcct ggcctactgg
      721 ttcatggacg acggcggcaa gtgggactac aacaagaaca gcaccaacaa gagcatcgtg
      781 ctgaacaccc agagcttcac cttcgag
//
