LOCUS       sequence-377398-        8352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter for SARS-CoV-2 5' UTR SL1 alone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   Shi M, Wang L, Fontana P, Vora S, Zhang Y, Fu TM, Lieberman J, Wu H
  TITLE     SARS-CoV-2 Nsp1 suppresses host but not viral translation through a 
            bipartite mechanism.
  JOURNAL   bioRxiv. 2020 Sep 18. doi: 10.1101/2020.09.18.302901.
  PUBMED    32995777
REFERENCE   2  (bases 1 to 8352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2020 Sep 18. doi: 10.1101/2020.09.18.302901."
COMMENT     Sequence Label: pLV-SARS-CoV-2 SL1 alone-Luciferase
FEATURES             Location/Qualifiers
     source          1..8352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1714..2017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2018..2221
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2172..2192
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2304..3956
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2370..2388)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    3969..4557
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4022..4042)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4440..4451)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4644..4877
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4949..5070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4986..5005)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5040..5059
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5110..5245
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5265..5284)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5266..5284)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5294..5311)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5294..5310)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5303..5325)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5452..5907
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5539..5558)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5749..5770
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5933..6037
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6038..6106,6107..6898)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6256..6275)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7069..7657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7558..7577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7811..7828
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7945..7966
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7981..7986,7987..8004,8005..8011)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8019..8035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8024..8046
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8043..8059
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8043..8059
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8078..8098
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8080..8098
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8126..8352
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtatagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc
     1741 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1801 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1861 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1921 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1981 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2041 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2101 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2161 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2221 tcgtttagtg aaccattaaa ggtttatacc ttcccaggta acaaaccatg gtgagcaagg
     2281 gcgaggcagt gggatcagga tcaatggaag acgccaaaaa cataaagaaa ggcccggcgc
     2341 cattctatcc gctggaagat ggaaccgctg gagagcaact gcataaggct atgaagagat
     2401 acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg gacatcactt
     2461 acgctgagta cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga
     2521 atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt
     2581 tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg
     2641 aattgctcaa cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt
     2701 tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg
     2761 attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac
     2821 ctcccggttt taatgaatac gattttgtgc cagagtcctt cgatagggac aagacaattg
     2881 cactgatcat gaactcctct ggatctactg gtctgcctaa aggtgtcgct ctgcctcata
     2941 gaactgcctg cgtgagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc
     3001 cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac
     3061 tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt
     3121 ttctgaggag ccttcaggat tacaagattc aaagtgcgct gctggtgcca accctattct
     3181 ccttcttcgc caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg
     3241 cttctggtgg cgctcccctc tctaaggaag tcggggaagc ggttgccaag aggttccatc
     3301 tgccaggtat caggcaagga tatgggctca ctgagactac atcagctatt ctgattacac
     3361 ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg
     3421 ttgtggatct ggataccggg aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga
     3481 gaggtcctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg
     3541 acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct
     3601 tcatcgttga ccgcctgaag tctctgatta agtacaaagg ctatcaggtg gctcccgctg
     3661 aattggaatc catcttgctc caacacccca acatcttcga cgcaggtgtc gcaggtcttc
     3721 ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga
     3781 tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc
     3841 gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa
     3901 gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagatcgcc gtgtaataca
     3961 aggtcgacaa tcaacctctg tattacaaaa tttgtgaaag attgactggt attcttaact
     4021 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4081 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4141 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4201 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4261 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4321 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     4381 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4441 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4501 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     4561 gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     4621 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     4681 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     4741 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     4801 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     4861 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     4921 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     4981 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5041 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     5101 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5161 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     5221 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     5281 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     5341 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     5401 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     5461 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5521 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5581 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5641 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     5701 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5761 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     5821 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5881 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     5941 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6001 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6061 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6121 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6181 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6241 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     6301 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6361 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6421 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6481 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6541 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6601 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6661 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6721 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6781 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6841 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6901 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6961 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7741 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7801 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7861 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     7921 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     7981 tttccacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     8041 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     8101 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     8161 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
     8221 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     8281 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     8341 cgatacataa ac
//
