LOCUS       sequence-37745-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: pCVL SFFV-EF1s HA.NLS.Sce(opt)
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        75..286
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      295..304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             310..336
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             310..336
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             346..366
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcggcgcgcc agtcctccga cagactgagt cgcccggatc gatcctcgag atccaggctc
       61 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
      121 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
      181 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
      241 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggatc tgccgccacc
      301 atgggcgtat acccctacga cgtgcccgac tacgcccccg ggccccctaa gaaaaagagg
      361 aaggtgaaga acatcaagaa gaaccaggtc atgaacctgg gccccaacag caagctgctg
      421 aaggagtaca agagccagct gatcgagctg aacatcgagc agttcgaggc cggcatcggc
      481 ctgatcctgg gcgacgccta catcaggagc agggacgagg gcaagaccta ctgcatgcag
      541 ttcgagtgga agaacaaggc ctacatggac cacgtgtgcc tgctgtacga ccagtgggtg
      601 ctgagccccc cccacaagaa ggagagggtg aaccacctgg gcaacctggt catcacctgg
      661 ggcgcccaga ccttcaagca ccaggccttc aacaagctgg ccaacctgtt catcgtgaac
      721 aacaagaaga ccatccccaa caacctggtg gagaactacc tgacccccat gagcctggcc
      781 tactggttca tggacgacgg cggcaagtgg gactacaaca agaacagcac caacaagagc
      841 atcgtgctga acacccagag cttcaccttc
//
