LOCUS       sequence-377521-        7148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pacr-2s-Arch::wCherry unc-54 3' UTR C.elegans A/B MN expression of 
            Arch RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Lu Y, Ahamed T, Mulcahy B, Meng J, Witvliet D, Guan SA, Holmyard D, 
            Hung W, Wen Q, Chisholm AD, Samuel ADT, Zhen M
  TITLE     Extrasynaptic signaling enables an asymmetric juvenile motor circuit
            to produce symmetric undulation.
  JOURNAL   Curr Biol. 2022 Nov 7;32(21):4631-4644.e5. doi: 
            10.1016/j.cub.2022.09.002. Epub 2022 Sep 30.
  PUBMED    36182701
REFERENCE   2  (bases 1 to 7148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.09.002"; journalName: "Curr Biol"; date: 
            "2022-11-7- 7"; volume: "32"; issue: "21"; pages: "4631-4644.e5"
COMMENT     Sequence Label: pJH3717
FEATURES             Location/Qualifiers
     source          1..7148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1993..2766
                     /codon_start=1
                     /product="light-driven proton pump archaerhodopsin-3 from 
                     Halorubrum sodomense (Chow et al., 2010)"
                     /label=Arch
                     /translation="MDPIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVPGIASAAYLSMFFGIGLTEVTVGGEMLDIYYARYADWLFTTPLLL
                     LDLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYFLA
                     TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGADVSAAD"
     primer_bind     complement(4588..4610)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4710..4729
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4807..4825)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4893..4997
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4998..5066,5067..5858)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5216..5235)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6029..6617
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6518..6537
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6771..6788
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6905..6926
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6941..6946,6947..6964,6965..6971)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6979..6995
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6984..7006
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7003..7019
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7003..7019
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagcatgc ggccgctcac caccgactga ttttcatttc aaaattaatg
       61 cagatttcta cgtgttcttt ttcaaataaa tttatatatt ttttgttctc tcacaaaata
      121 aataatttcg agagttaagt gatcattctc agacatattc tataattgtc cgtcaggaaa
      181 cagatagagg aacctttgat aaataaatta taaaaggccg acagggaaat gcttctccct
      241 cgactctgtt agaagcgaag aggcaatttt taaaaatttc ccatttttat gcgtttttct
      301 gatttccaaa gtcacgcatc atttaccaac cgcaacgatt cataaggacg taattttgat
      361 tttctggacg atttttatat agttttgatg tgaaattcgc tttatttgtc tgaaagtgga
      421 atagatttag tgtggattta accgaaaaat gcagatcttt taaaaattta aaccagtgtt
      481 ttgttgcctt atttgcgaaa aaaaattgtg atatttcgag tattgctgca atttgattag
      541 taaaatgtta ggatataagt atttaaaaag tataattggt tgaattttct ttttcatcac
      601 cgacttccaa aaatatagag atactgagtt ttcaccattt tttgaatgta aataaaaaca
      661 ttttgaacac ttgtgctggg attcaatcca aatacattca ctgtcattta atattattat
      721 atatcaaaat aaaacgcata actttttctg ttttttttta aattttttca aaatgtaggt
      781 ttggtaagtt aaggtaaggt taggcaatgt aggtaaacac gagatgatac tccacgttga
      841 aaattgtttt cataaataca gtttagattg aactgaagga ttttatcgtc taatagaata
      901 taatttttta gttcaagatt taggatttgt tttcactttt ttttagattc aagacatata
      961 tctcgcaaaa tgagttttgt ctttgagcca tcttgtcatc atagcacatt gaattttgtt
     1021 gttcgcaaga cggaatatat gttcccagct gcaaattttg tgtcggatat ttcttcttta
     1081 gcgaaaggcg ttaaaaacca aaaatagtcg aagcgtttga tatgtgtccc gttggttttt
     1141 cgtgtccatc tattctgttt cgagtacaca tgttgcgacg cggcactgat gaaaaagtga
     1201 aagagctagt gtcacacaca aacacaaagt atgctcatgt gagtgtgtag atatgtgtag
     1261 atgtatgtcc tgaaggaata aaaagacttt ttgaagctac atttttaaac aaaaaaagac
     1321 agaaacatga agcacaactc tctattctgt cgaacggcgt gtgcaactca cgcacaccca
     1381 aaaatatgaa atataacatt cgcaccaaca aagcgcgaat atcaatgaat ctcgagggaa
     1441 cgatggctgc ctgccggcgc gtgttgctct tctgtcattg ggataactgg cgggtctgtt
     1501 gggtccgtcc tttagaccat ttgactacac tgccctgcat atgtgtgaaa cgtcgtcgtt
     1561 gctgttggga gctgtctgtg tgtatgtgtg tgtgtgtgag tatggagcag taaaaagctg
     1621 ccgatacgag aagcgccgcc ccggacggac ggcgatggcg catcgccaaa tgtgtcattt
     1681 tagttgtctt cacgaaccag agggcccacg acggacgggc ggccacgccc acctcaacca
     1741 gaatatcctt ccttctccca cccacccttg cggacgcgcg gatgcgctga acaaaaagac
     1801 gacgaagaag aagaagaaga agctgaacga agaaaaaagc atcaaaagaa gtgaagcggc
     1861 tctaccaccc cgaccacctt ccttcacagg aaggacgccg ttttcatccc gggattggcc
     1921 aaaggaccca aaggtatgtt tcgaatgata ctaacataac atagaacatt ttcaggagga
     1981 cccttggcta gcatggaccc catcgctctg caggctggtt acgacctgct gggtgacggc
     2041 agacctgaaa ctctgtggct gggcatcggc actctgctga tgctgattgg aaccttctac
     2101 tttctggtcc gcggatgggg agtcaccgat aaggatgccc gggaatatta cgctgtgact
     2161 atcctggtgc ccggaatcgc atccgccgca tatctgtcta tgttctttgg tatcgggctt
     2221 actgaggtga ccgtcggggg cgaaatgttg gatatctatt atgccaggta cgccgactgg
     2281 ctgtttacca ccccacttct gctgctggat ctggcccttc tcgctaaggt ggatcgggtg
     2341 accatcggca ccctggtggg tgtggacgcc ctgatgatcg tcactggcct catcggagcc
     2401 ttgagccaca cggccatagc cagatacagt tggtggttgt tctctacaat ttgcatgata
     2461 gtggtgctct attttctggc tacatccctg cgatctgctg caaaggagcg gggccccgag
     2521 gtggcatcta cctttaacac cctgacagct ctggtcttgg tgctgtggac cgcttaccct
     2581 atcctgtgga tcataggcac tgagggcgct ggcgtggtgg gcctgggcat cgaaactctg
     2641 ctgtttatgg tgttggacgt gactgccaag gtcggctttg gctttatcct gttgagatcc
     2701 cgggctattc tgggcgacac cgaggcacca gaacccagtg ccggtgccga tgtcagtgcc
     2761 gccgaccggg taccgggagg aggaaccatg gtctcaaagg gtgaagaaga taacatggca
     2821 attattaaag agtttatgcg tttcaaggtg catatggagg gatctgtcaa tgggcatgag
     2881 tttgaaattg aaggtgaagg agaaggccga ccatatgagg gaacacaaac cgcaaaacta
     2941 aaggtaagtt taaacatata tatactaact aaccctgatt atttaaattt tcaggtaact
     3001 aaaggcggac cattaccatt cgcctgggac atcctctctc cacagttcat gtatggaagt
     3061 aaagcttatg ttaaacatcc ggcagatata ccagattatt tgaaactttc attcccggag
     3121 ggttttaagt gggaacgcgt aatgaatttt gaagacggag gagttgttac agtgacgcaa
     3181 gactcaaggt aagtttaaac agttcggtac taactaacca tacatattta aattttcagc
     3241 ctccaagatg gagaatttat ttataaagtc aaacttcgag gaacgaattt cccctcggat
     3301 ggatctgtta tgcagaagaa gactatggga tgggaagctt caagtgaaag aatgtaccct
     3361 gaagacggtg ctcttaaggg agagattaaa caacgtctta aattgaaaga tggaggacat
     3421 tacgatgctg aggtaagttt aaacatgatt ttactaacta actaatctga tttaaatttt
     3481 caggtgaaga caacttacaa agccaaaaaa ccagttcagc tgccaggagc gtacaatgtt
     3541 aatattaaac tggatatcac ctcccacaac gaggattaca ctatcgttga gcaatatgaa
     3601 agagctgaag ggcggcactc gacaggtggc atggatgaat tgtataagtt ctgctacgag
     3661 aacgaggtct aagaattcca actgagcgcc ggtcgctacc attaccaact tgtctggtgt
     3721 caaaaataat aggggccgct gtcatcagag taagtttaaa ctgagttcta ctaactaacg
     3781 agtaatattt aaattttcag catctcgcgc ccgtgcctct gacttctaag tccaattact
     3841 cttcaacatc cctacatgct ctttctccct gtgctcccac cccctatttt tgttattatc
     3901 aaaaaacttc tcttaatttc tttgtttttt agcttctttt aagtcacctc taacaatgaa
     3961 attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc
     4021 ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg tacacttctt
     4081 atgtttttta cttctgataa atttttttga aacatcatag aaaaaaccgc acacaaaata
     4141 ccttatcata tgttacgttt cagtttatga ccgcaatttt tatttcttcg cacgtctggg
     4201 cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt ttggaatttt
     4261 tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt tgctttttgg ggtttcccct
     4321 attgtttgtc aagatttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag
     4381 cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga
     4441 agttgataat ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac
     4501 attcccaata taccaaacat aactgtttcc tactagtcgg ccgtacgggc cctttcgtct
     4561 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     4621 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     4681 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     4741 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcggcc
     4801 ttaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     4861 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4921 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4981 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5041 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5101 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5161 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5221 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5281 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5341 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5401 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5461 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5521 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5581 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5641 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5701 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5761 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5821 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5881 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5941 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6001 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6061 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6121 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6181 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6241 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6301 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6361 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6421 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6481 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6541 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6601 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6661 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6721 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6781 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     6841 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     6901 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     6961 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7021 atgattacgc caagctgtaa gtttaaacat gatcttacta actaactatt ctcatttaaa
     7081 ttttcagagc ttaaaaatgg ctgaaatcac tcacaacgat ggatacgcta acaacttgga
     7141 aatgaaat
//
