LOCUS       sequence-377546-        8109 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc-4-TeTx::mCherry unc-54 3' UTR C.elegans A MNs/others neurou 
            expression of TeTx RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Lu Y, Ahamed T, Mulcahy B, Meng J, Witvliet D, Guan SA, Holmyard D, 
            Hung W, Wen Q, Chisholm AD, Samuel ADT, Zhen M
  TITLE     Extrasynaptic signaling enables an asymmetric juvenile motor circuit
            to produce symmetric undulation.
  JOURNAL   Curr Biol. 2022 Nov 7;32(21):4631-4644.e5. doi: 
            10.1016/j.cub.2022.09.002. Epub 2022 Sep 30.
  PUBMED    36182701
REFERENCE   2  (bases 1 to 8109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.09.002"; journalName: "Curr Biol"; date: 
            "2022-11-7- 7"; volume: "32"; issue: "21"; pages: "4631-4644.e5"
COMMENT     Sequence Label: pJH2685
FEATURES             Location/Qualifiers
     source          1..8109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4053..4763
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4198..4216)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4466..4485
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5679..5701)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5801..5820
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5902..5920)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5988..6092
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6093..6161,6162..6953)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6311..6330)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7124..7712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7613..7632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7866..7883
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8000..8021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8036..8041,8042..8059,8060..8066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8074..8090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8079..8101
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(8098..8109,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(8098..8109,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcg cggccgcttc cccaaattgg aacagtgaaa
       61 tactcaatca atcactctgg cggttttctc cacaaagaac acgaaattgg aaaagggaaa
      121 agattgattt gtttgaaggc gtgaggaaat cgaaaaaaaa atgtttcaaa acaaatgaaa
      181 tattttaatg aataaaccca ggaatgccac cttttggtgc ccgcctacaa aaaagtcaac
      241 tgttcttaaa ctcctacata gttgccctct ctaacgaatg ttgctttttt taaattttca
      301 ttgaatttga ttatcgtctg tatgcgccta ctctatcagt ggaaaacatg cccatttccc
      361 acaaatttac tcgtttggag ctcaaaatgt taaatggggc atcatggaat tggcatctca
      421 cagaaagtgg cggatgagtt atatgtaata ataatggtgg ctgaagtgga aaccgcaaaa
      481 aaaggatgtg tatttgagat tttgtaacgg aatttgaatc agatacacaa aatgagagaa
      541 atttatagat tctttaaaat ccacctgaag tcggcaaatt tttgaaaggt ctgttcgttc
      601 agtttttcat aagttttatg aattttgatt tactactgaa gttcgagaaa agccctttaa
      661 ccataccaat aagaaattat gttaacttta caagaatatt aacaaaaaat tattaaaaaa
      721 ctgtgttccc ctggcattat gatatttcca ccgtcagttc tctattaaaa cttcaactct
      781 acacagtttc aactctcaaa tgagctgtcc atcttttgcc ttcaggaaat tatgctaatc
      841 tgctgatttc gcgcactttc accacactat ttccaaatag aaggaggggt ggtgcaacac
      901 acgagacact gcaaaaaggt tcagtgaaga tttatctact tgtgagatga gagcaggagg
      961 ggtgggagaa gtgtgatgat tgacgggatg acaagaacag tgatgtggtg tcgaaatgga
     1021 agatgcgcta atttgtgggg ttttatgaaa tttgaataat tacatttata acatctacta
     1081 gttaggcgtc tgtgtaggta ttgcctgcct gcctacaagg catatgtacg aggcaggtta
     1141 aacctcctag aaagctacgg tactttcaaa ccacataaca tttatactgg tgtcttttcg
     1201 cgtcttcttc ttgaacatgt agaccctcaa aaagctaagt tttacactgt gtagtttgct
     1261 ttgaaatcta caaaattgga ggcacgcctt ctggactttg ccgacacgca tactttctac
     1321 ctaccaactg cctacttcca cagtctagcg tgaactccaa taccatttta aaaattgttt
     1381 tatctcaaat tttcaatttt taaatccaat ttcccagttc taaaaaaaaa attttttttt
     1441 taatttaaaa actctcatcc tttttaatgc tcgttcccat tcggcaagac gtgtaccaaa
     1501 cacaaaacaa attgactggt gatccatctc gaatttctcg tttcaaatta gtcgttgcta
     1561 tatatattgt cttatgtgtg gaaaacgaat aagaaaaatt agagaaagat gagagagcag
     1621 gggcagaatg aaaatttgca tggaaattga agaagatcac tgctttttcc gatgaatgat
     1681 gactaaatag aatgagttga gaggaaaata atcataattc taattttaat tagaaatatg
     1741 atatggaaag taagcaaaac cacaaggctt ttgctctgaa cttatttccg aaaaaaaaaa
     1801 gtgaaactga aagttagaat tttcaaaata atattgcgag agtcaacctt cagaaaaatt
     1861 ggcgagaaat caaatttgaa ttctctgaaa atttagttaa aatacatatg ttatccttct
     1921 ttgttttttg gccttagcct gccttcaagg cgacctgagc ctatgcgggt gcctaatacc
     1981 gaaacacaca taattttttt ttttaatctt taaatactgt aacgagtcgc gagatccagc
     2041 gagacccata gaatatgttg agcgctgcaa acaatcgtat aactcttgtt caaatttttc
     2101 cactctgtat ttttgcaagc agttcaacaa atggtcatca tttttataac ccatatctat
     2161 attcgaatct gacataacaa ataatactaa gcacatcctc ccacatctct tttcattcaa
     2221 gttgcgcaat tcccctaata actatggatg gaggggggac cgagcccaaa gattattcaa
     2281 aacgataacg actttttcat gcggccaacg aaaaataaaa cttttctcat attctcctat
     2341 ttcccatttc aagaattaca tccccatggg acgttgaaat cacatcatat tagtatatgt
     2401 taatagttct tttcactgga atatcgccac gccccccttc cggtggctca ttggtttcga
     2461 gaagaaggcg gagccgaagt gggttcttct ttcttttttt gtggtagaag tctagcagga
     2521 ttaagctact attccattag aggatcttct tcttccaaaa agtgaaaatc ccgggattgg
     2581 ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag
     2641 gacccttggc tagcatgccg atcaccatca acaacttccg ttactccgac ccggttaaca
     2701 acgacaccat catcatgatg gaaccgccgt actgcaaagg tctagacatc tactacaaag
     2761 ccttcaaaat caccgaccgt atctggatcg ttccggaacg ttacgaattt ggtactaaac
     2821 cggaagactt caacccgccg tcctccctga tcgaaggtgc ttccgaatac tacgacccga
     2881 actacctgcg taccgactcc gacaaagacc gtttcctgca gaccatggtt aaactgttca
     2941 accgtatcaa aaacaacgtt gctggtgaag ctctgctgga caaaatcatc aacgctatcc
     3001 cgtacctggg taactcctac tccctgctgg acaaattcga caccaactcc aactccgttt
     3061 ccttcaacct gctggaacag gatccgtccg gtgctaccac caaatccgct atgctgacca
     3121 acctgatcat cttcggtccg ggtccggttc tgaacaaaaa cgaagttcgt ggtatcgttc
     3181 tgcgtgttga caacaaaaac tacttcccgt gccgtgacgg tttcggttcc atcatgcaga
     3241 tggctttctg cccggaatac gtaccgacct tcgacaacgt tatcgaaaac atcacctccc
     3301 tgaccatcgg taaatccaaa tacttccagg acccggctct gctgctgatg cacgaactga
     3361 tccacgttct gcacggtctg tacggcatgc aggtttcctc ccacgaaatc atcccgtcca
     3421 aacaggaaat ctacatgcag cacacctacc cgatctccgc tgaagagctc ttcaccttcg
     3481 gtggtcagga cgctaacctg atctccatcg atatcaaaaa cgacctgtac gaaaaaaccc
     3541 tgaacgacta caaagctatc gctaacaaac tgtcccaggt tacctcctgc aacgacccga
     3601 acatcgacat cgactcctac aaacagatct atcagcagaa gtatcagttt gataaggatt
     3661 ccaacggcca gtatatagtg aacgaggata agtttcagat actgtataac tccataatgt
     3721 atggctttac tgagatagag ctgggcaaga agtttaacat aaagactcgc ttaagctatt
     3781 tttccatgaa ccacgatcct gtgaagatac ctaacctgct ggatgatact atatataacg
     3841 atactgaggg ctttaacata gagtccaagg atctgaagtc cgagtataag ggccagaaca
     3901 tgagggtgaa cactaacgcc tttaggaacg tcgacggttc cggtctggtt tccaaactga
     3961 tcggtctgtg caaaaaaatc atcccgccga ccaacatccg tgaaaacctg tacaaccgta
     4021 ccgctagggt accggtagaa aaaatgctcg agatggtgag caagggcgag gaggataaca
     4081 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     4141 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     4201 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     4261 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     4321 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     4381 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     4441 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     4501 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     4561 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     4621 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     4681 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     4741 gcatggacga gctgtacaag taagaattcc aactgagcgc cggtcgctac cattaccaac
     4801 ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa actgagttct
     4861 actaactaac gagtaatatt taaattttca gcatctcgcg cccgtgcctc tgacttctaa
     4921 gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt
     4981 ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct
     5041 ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa
     5101 aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt
     5161 gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg
     5221 cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc
     5281 gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt
     5341 tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg
     5401 gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa
     5461 gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga
     5521 aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa
     5581 tgacagaata cattcccaat ataccaaaca taactgtttc ctactagtcg gccgtacggg
     5641 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     5701 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     5761 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     5821 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     5881 atcaggcggc cttaattaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     5941 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6001 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6061 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6121 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6181 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6241 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6301 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6361 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6421 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6481 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6541 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6601 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6661 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6721 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6781 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6841 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6901 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6961 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7021 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7081 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7141 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7201 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7261 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7321 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7381 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7441 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7501 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7561 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7621 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7681 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7741 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7801 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7861 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     7921 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     7981 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     8041 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     8101 gaaacagct
//
