LOCUS       sequence-377549-        7729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc-17-Cre unc-54 3'UTR C.elegans for Cre/Lox .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Lu Y, Ahamed T, Mulcahy B, Meng J, Witvliet D, Guan SA, Holmyard D, 
            Hung W, Wen Q, Chisholm AD, Samuel ADT, Zhen M
  TITLE     Extrasynaptic signaling enables an asymmetric juvenile motor circuit
            to produce symmetric undulation.
  JOURNAL   Curr Biol. 2022 Nov 7;32(21):4631-4644.e5. doi: 
            10.1016/j.cub.2022.09.002. Epub 2022 Sep 30.
  PUBMED    36182701
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.09.002"; journalName: "Curr Biol"; date: 
            "2022-11-7- 7"; volume: "32"; issue: "21"; pages: "4631-4644.e5"
COMMENT     Sequence Label: pJH4143
FEATURES             Location/Qualifiers
     source          1..7729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3381..3401
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3402..4430
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     primer_bind     complement(5299..5321)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5421..5440
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5522..5540)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5608..5712
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5713..5781,5782..6573)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5931..5950)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6744..7332
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7233..7252
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7486..7503
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7620..7641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7656..7661,7662..7679,7680..7686)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7694..7710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7699..7721
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(7718..7729,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(7718..7729,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgct cccggccgcc atggccgcgg gattacacca
       61 atcatttctc ccctccgaaa tgtgtagtcg tcatgacaaa gtggtgacac tggattacgg
      121 taggctccgc ccacttcttg gaaaagtgac gtgggctcgg tgagccctaa agcttttggt
      181 tttcacaatt ttctggtttt tgggattttt gcgggaattt tgagaggaga aagttgggag
      241 agaagatatt ggaatggtcc ccggggagct gtacatttat acatctagac tgaagctggg
      301 gtattgcaaa aagagaaaag cctatcactt tctttccatc ctgcatactt gccaaaaagt
      361 tttggctgag cttattttta aaattggaat tttttggaat tttcagaaaa gaaaaagttt
      421 tttctttttt tttaatttaa aaaatttttg ctatttattg catggtacta cagaaaaact
      481 tgaatgaaat aaaaaatttg gaaaattcaa gaaaactttt tttttcatag tttatttatt
      541 cgagttttaa tgtggctttt attttgttcg gtttttgttt taaaaagatt ttatacaaaa
      601 tttcaagaat ttacaatttt tccgttgtaa aaaatccaaa aaaaaatcca aaaaaatagt
      661 tcagaccttt ttttaaattt taaatttaaa aattcaatac caaaaaatta aaaaaaatta
      721 gtttttccga gaataaagga aaaatgttga aaaaatatta tatataaaat ttccagaaaa
      781 tgtgaaaact ccaaaaaact gcagactttt ccccaaacta gctatttttt gaattcatta
      841 cctttaaagg tggagtaccg aaatttaaga ctttgctttt ttagacccaa aatggtccaa
      901 aactaccaaa tttcgtaatg agacgttctg aaaatttttc aaaaaaatgt tatgggagct
      961 caaagttttt gaaaaaatgg tatattttta gctaatttta accacttctt ggcgtcgcag
     1021 cggttggaac tcaattttaa ttaaattatc atccttcaat gtccttcaat ctttgcaatt
     1081 tcaatcgacc atacgcgttc agagcatttt tttttttcgt ttttcctccc gtccagcaca
     1141 tatttaaata ttctctctcc ccctcccctg gttcctaaaa acttgggaaa ggggaatttc
     1201 gaaggaggtg gaagcttctt cttctccccg agcacacaca cacatcctct cttcaaaatc
     1261 tttaagaaat agtgcgtgtg aatgactaga cgctgaagct aacttggttt ccgcgaacaa
     1321 tcccaatcag tgcctgtgcc gaggcttcgg agaagaagag attgacgtcg tctttttctt
     1381 tttttgtgaa acagaataaa aaagaatacg aaatggactt tttcacacat tttcgttact
     1441 tttccagaaa aaaacgaagc tctagccatg gaagtttgaa ttgaaaacct aaaccccgat
     1501 gtgtattcat ttgagcttaa gcctaagcct aattctacgc ctaagcataa gcctaagcct
     1561 atgttcaagc ctaagccaaa ttgtagaacg ttaccaaaag atcaaaccta tttttcctac
     1621 agaaaaggtc taatgataac tgttaagcga gagacaagga gagatatata tcaaaaacta
     1681 gcccaaactg tgattaacat ctattaactg acctcaaaaa aaaaaatagt agagattaaa
     1741 tactgctcaa aaagtttctt ggaaaagtaa attgttaggt ttctggggcc tgtaaaactg
     1801 gaccgagttt tgaagaattt ttttaacttt tcaaagtttg gcgtcactga aagctttata
     1861 ttgtacatgt tccaccaatt ttcttttgta tccgtttttt ctgtctcaaa acggagctcg
     1921 agcccatgac gtgtggatac cgtgagaatc cgcccaattc cttcacatcg atctcatatt
     1981 tctttcccag tttatattga attcccgttg aattgcggct ttgcagggga gaagatgtgc
     2041 gtctttatat ttttttttct atccttaccc gccgtgaatc cggctcgaat tgagccaatt
     2101 tttatgaata gtagagctaa ttgacttcgt ctccctagaa ttgatgaggt aaagctcgtg
     2161 gggagatatg gagatttttc gatttttttt ccagtaattt tcagttcaaa atttgagctt
     2221 ttgagcagga gttttgagat ttgtgccaaa atttcctatt attttttcaa atatttttgc
     2281 ttttgtaaaa aaggtgtagt agaagttttg tagtgccact attttttttt tgaaaattat
     2341 gttttggcgg aaagttcttt aaaaaagtta atcaaaattt ttggcgcttt agtcaaatta
     2401 attttctggc aaaaatatta gtaattttgg cgtgagttca attttcccat tcttaaaatt
     2461 tcacacaatt aagaatttta agatttgggt ttttttttca acaaaaaatc aaatatttac
     2521 atgaaaattc ggcgggaaat aaaaaaaaat attccgattc aatgtgtgaa taactttttg
     2581 cggggaattc aaatttgcat tatttaaact ttttttaagc tcgaaaaatt gaaattctta
     2641 aataaataaa taaaattttc ctcgaatttt gaatgtttcg attttttagc gttatttttt
     2701 caaaatttcc actaaaactc tagttctaaa gttctaaaac tttaaaaact tcctaatgtt
     2761 gtggtcttgt gtttttttgg cagcgatttc caaatttctg aaattccaac aaaaaaacca
     2821 ctacgtcagt ttgcatatcc aacccccccc cccccccccc cccctctgag ccaatcaatc
     2881 atgtgcattt tgaattggcg ccaaggcaat cttcgtaaaa aataacaata gagtcatccc
     2941 tcagaacaga aaagaaaccc gcccgggggc tcaaatacac atgtcatttt tcttttttcg
     3001 cctgattttt agcctaaatt agtgtttagc aattttttaa ggtaactcgg tggagtcatt
     3061 tgaacttttt aaactaaaat ttcagaaaaa attctttttt tttgtcaaaa aataattttc
     3121 ccgccggaat ttttcaaaaa aataattttt attttatttt tcaagttcct tgtttcctct
     3181 ttattttttt attttgcccc caaaagtttt acactaaaat atttaatttt caggtaattc
     3241 atcgaaagtc tttctatttt ccgcatctct tgttcaaggg attggccaaa ggacccaaag
     3301 gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc ttggctagcg
     3361 tcgacggtac catgggcgca cccaagaaga agaggaaggt gtccaattta ctgaccgtac
     3421 accaaaattt gcctgcatta ccggtcgatg caacgagtga tgaggttcgc aagaacctga
     3481 tggacatgtt cagggatcgc caggcgtttt ctgagcatac ctggaaaatg cttctgtccg
     3541 tttgccggtc gtgggcggca tggtgcaagt tgaataaccg gaaatggttt cccgcagaac
     3601 ctgaagatgt tcgcgattat cttctatatc ttcaggcgcg cggtctggca gtaaaaacta
     3661 tccagcaaca tttgggccag ctaaacatgc ttcatcgtcg gtccgggctg ccacgaccaa
     3721 gtgacagcaa tgctgtttca ctggttatgc ggcggatccg aaaagaaaac gttgatgccg
     3781 gtgaacgtgc aaaacaggct ctagcgttcg aacgcactga tttcgaccag gttcgttcac
     3841 tcatggaaaa tagcgatcgc tgccaggata tacgtaatct ggcatttctg gggattgctt
     3901 ataacaccct gttacgtata gccgaaattg ccaggatcag ggttaaagat atctcacgta
     3961 ctgacggtgg gagaatgtta atccatattg gcagaacgaa aacgctggtt agcaccgcag
     4021 gtgtagagaa ggcacttagc ctgggggtaa ctaaactggt cgagcgatgg atttccgtct
     4081 ctggtgtagc tgatgatccg aataactacc tgttttgccg ggtcagaaaa aatggtgttg
     4141 ccgcgccatc tgccaccagc cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa
     4201 ctcatcgatt gatttacggc gctaaggatg actctggtca gagatacctg gcctggtctg
     4261 gacacagtgc ccgtgtcgga gccgcgcgag atatggcccg cgctggagtt tcaataccgg
     4321 agatcatgca agctggtggc tggaccaatg taaatattgt catgaactat atccgtaacc
     4381 tggatagtga aacaggggca atggtgcgcc tgctggaaga tggcgattag tctagaatca
     4441 ctagtgaatt cgcggccgcc tgcaggtcga ccatatggga gagctccgca tcggccgctg
     4501 tcatcagatc gccatctcgc gcccgtgcct ctgacttcta agtccaatta ctcttcaaca
     4561 tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaact
     4621 tctcttaatt tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta
     4681 gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc
     4741 attaataata attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt
     4801 tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca
     4861 tatgttacgt ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat
     4921 gacgtcaaat catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa
     4981 gtgaaagttt atgaaattaa ttttcctgct tttgcttttt ggggtttccc ctattgtttg
     5041 tcaagatttc gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc
     5101 aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata
     5161 atttgaaagt ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa
     5221 tataccaaac ataactgttt ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt
     5281 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     5341 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     5401 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     5461 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcggc cttaattaag
     5521 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     5581 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5641 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5701 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5761 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5821 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5881 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5941 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6001 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6061 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6121 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6181 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6241 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6301 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6361 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6421 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6481 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6541 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6601 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6661 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6721 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6781 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6841 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6901 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6961 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7021 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7081 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7141 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7201 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7261 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7321 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7381 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7441 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7501 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7561 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7621 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     7681 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagct
//
