LOCUS       sequence-377550-        6435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pttr-39-Chrimson::wCherry unc-54 3' UTR C.elegans D motor neurou 
            expression of Chrimson RFP .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Lu Y, Ahamed T, Mulcahy B, Meng J, Witvliet D, Guan SA, Holmyard D, 
            Hung W, Wen Q, Chisholm AD, Samuel ADT, Zhen M
  TITLE     Extrasynaptic signaling enables an asymmetric juvenile motor circuit
            to produce symmetric undulation.
  JOURNAL   Curr Biol. 2022 Nov 7;32(21):4631-4644.e5. doi: 
            10.1016/j.cub.2022.09.002. Epub 2022 Sep 30.
  PUBMED    36182701
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.09.002"; journalName: "Curr Biol"; date: 
            "2022-11-7- 7"; volume: "32"; issue: "21"; pages: "4631-4644.e5"
COMMENT     Sequence Label: pJH3557
FEATURES             Location/Qualifiers
     source          1..6435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1592..1603
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             2249..2956
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2391..2409)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2659..2678
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(3875..3897)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3997..4016
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4094..4112)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4180..4284
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4285..4353,4354..5145)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4503..4522)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5316..5904
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5805..5824
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6058..6075
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6192..6213
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6228..6233,6234..6251,6252..6258)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6266..6282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6271..6293
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6290..6306
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6290..6306
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagcatgc ggccgccctc ttctttctta tcgacagcca ttattatttc
       61 tatcggctac ttttttcttt ttagctgcct tctgctacgc ggagaattgg aaattttcga
      121 aactgatttt gaatatcgat tctgattgta tttctgataa agacgtggaa agttcgaagg
      181 gtcggattac ggggtatttc aaaggttaca cgacttaaat ttaaatagtt ttatctattc
      241 tcgttggcac gaagtatatc taggcaatcg tgacgaagga aacctttgac gtttgtgtag
      301 agaaaagcaa actattctgg aacacttcaa aagatctcta aaatacaaat atcgaatttg
      361 tatttttgta tgaaaaagtg gattaaaagg gtgttcgctt cgagaaagat acaaaatgct
      421 gaaattagta tatagaagtt cctaaagttt gaaagcagag ccaagcgtta ctgaatttgg
      481 aaaaaggaaa tttcctgcat aggtgaagcc taataggtga aatggatgta acatgaaaat
      541 gttctaaaac attttcaccg taaaatttag gtaggcggga aggtcctgtt gtgcccatat
      601 aggcttatga tctctctgaa aaactctctg aactttgaat ttctccagaa tgtttttggt
      661 ttctgatcga taaatgttta ctggttgttg atgtttgtaa aagtcctgca aattctagaa
      721 aattctcaaa ctctttttct tattttcatg agaaaaccga aactttgaag tacataagca
      781 aagagttgcg ttgttatttt aatcttttga acctattaat aaatcttttt tgttttctcg
      841 ggattatgtt aggattacaa agacatttaa gacaagaagt tgtttctgtt atttcacttt
      901 ttttgtttgc gcattcacaa ttttgttacc cgggattggc caaaggaccc aaaggtatgt
      961 ttcgaatgat actaacataa catagaacat tttcaggagg acccttggct agcgtcgacg
     1021 gatccctatg gctgagctca tctcctccgc tacccgttcc ctcttcgctg ctggaggaat
     1081 caacccatgg ccgaacccat accaccacga ggacatggga tgcggaggaa tgaccccaac
     1141 cggagagtgc ttctccaccg agtggtggtg cgacccatcc tacggactct ccgacgctgg
     1201 atacggatac tgcttcgtcg aggctaccgg aggtaagttt aaacttggac ttactaacta
     1261 accatacata tttaaatttt caggatacct cgtcgtcgga gtcgagaaga agcaggcttg
     1321 gctccactcc cgtggaaccc caggagagaa gatcggagct caggtctgcc agtggatcgc
     1381 tttctccatc gctatcgctc tcctcacctt ctacggattc tccgcttgga aggctacctg
     1441 cggatgggag gaggtctacg tctgctgcgt cgaggtcctc ttcgtcaccc tcgagatctt
     1501 caaggagttc tcctccccag ctaccgtcta cctctccacc ggaaaccacg cttactgcct
     1561 ccgttacttc gagtggctcc tctcctgccc agtcatcctc atccgtctct ccaacctctc
     1621 cggactcaag gtaagtttaa acagttcggt actaactaac cctgattatt taaattttca
     1681 gaacgactac tccaagcgta ccatgggact catcgtctcc tgcgtcggaa tgatcgtctt
     1741 cggaatggct gctggactcg ctaccgactg gctcaagtgg ctcctctaca tcgtctcctg
     1801 catctacgga ggatacatgt acttccaggc tgctaagtgc tacgtcgagg ctaaccactc
     1861 cgtcccaaag ggacactgcc gtatggtcgt caagctcatg gcttacgctt acttcgcttc
     1921 ctggggttcc tacccaatcc tctgggctgt cggaccaggt aagtttaaac atatatatac
     1981 taactaacgg attatattta aattttcaga gggactcctc aagctctccc catacgctaa
     2041 ctccatcgga cactccatct gcgacatcat cgctaaggag ttctggacct tcctcgctca
     2101 ccacctccgt atcaagatcc acgagcacat cctcatccac ggagacatcc gtaagaccac
     2161 caagatggag atcggaggag aggaggtcga ggtcgaggag ttcgtcgagg aggaggacga
     2221 ggacaccgtc gcggtacccg cggccgccgt gagcaagggc gaggaggata acatggccat
     2281 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     2341 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     2401 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     2461 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     2521 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     2581 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     2641 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     2701 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     2761 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     2821 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     2881 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     2941 cgagctgtac aagtaatagg aattccaact gagcgccggt cgctaccatt accaacttgt
     3001 ctggtgtcaa aaataatagg ggccgctgtc atcagagtaa gtttaaactg agttctacta
     3061 actaacgagt aatatttaaa ttttcagcat ctcgcgcccg tgcctctgac ttctaagtcc
     3121 aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt
     3181 tattatcaaa aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa
     3241 caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt
     3301 gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac
     3361 acttcttatg ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca
     3421 caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac
     3481 gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg
     3541 gaatttttca atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggt
     3601 ttcccctatt gtttgtcaag atttcgagga cggcgttttt cttgctaaaa tcacaagtat
     3661 tgatgagcac gatgcaagaa agatcggaag aaggtttggg tttgaggctc agtggaaggt
     3721 gagtagaagt tgataatttg aaagtggagt agtgtctatg gggtttttgc cttaaatgac
     3781 agaatacatt cccaatatac caaacataac tgtttcctac tagtcggccg tacgggccct
     3841 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     3901 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     3961 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     4021 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     4081 ggcggcctta agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4141 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4201 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4261 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4321 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4381 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4441 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4501 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4561 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4621 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4681 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4741 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4801 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4861 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4921 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4981 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5041 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5101 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5161 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5221 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5281 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5341 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5401 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5461 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5521 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5581 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5641 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5701 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5761 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5821 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5881 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5941 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6001 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6061 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     6121 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     6181 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     6241 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     6301 tatgaccatg attacgccaa gctgtaagtt taaacatgat cttactaact aactattctc
     6361 atttaaattt tcagagctta aaaatggctg aaatcactca caacgatgga tacgctaaca
     6421 acttggaaat gaaat
//
