LOCUS       sequence-377554-        8896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression of ZER1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   Nouel J, White EA
  TITLE     ZER1 Contributes to the Carcinogenic Activity of High-Risk HPV E7 
            Proteins.
  JOURNAL   mBio. 2022 Nov 8:e0203322. doi: 10.1128/mbio.02033-22.
  PUBMED    36346242
REFERENCE   2  (bases 1 to 8896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2022 
            Nov 8:e0203322. doi: 10.1128/mbio.02033-22."
COMMENT     Sequence Label: MSCV-neo C-HAonly ZER1
FEATURES             Location/Qualifiers
     source          1..8896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     1417..1436
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1417..1435
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1465..1489
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3788..3812)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3859..3885
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3859..3885
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        3898..4397
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3933..3956)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4330..4349
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4418..5212
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4418..5212
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4472..4491)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5082..5101
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             5291..5805
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5967..5983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5991..5997,5998..6015,6016..6021))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6036..6057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6174..6191)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6345..6933)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6425..6444)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7104..7895,7896..7964))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7727..7746
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7965..8069)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8137..8155
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8193..8215)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8315..8334
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8528..8550
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8678..8697
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatctaat acgactcact atagggagac
     1441 ccaagctggc tagttaagct atcaacaagt ttgtacaaaa aagttggcat ggcgtccgac
     1501 actcccgagt cgctgatggc cctctgtact gacttctgct tgcgcaacct ggatggcacc
     1561 ctgggctacc tgctggacaa ggagaccctg cggctacatc cggacatctt cttgcccagc
     1621 gagatctgtg accggctcgt caatgagtat gtggagctgg tgaacgctgc ctgtaacttc
     1681 gagccacacg agagcttctt cagcctcttt tcggaccccc gcagcacccg cctcacgcgg
     1741 atccacctcc gtgaggacct ggtgcaggac caggacctgg aggccatccg caagcaggac
     1801 ctggtggagc tgtacctgac taactgcgag aagctgtccg ccaagagcct gcagacactg
     1861 aggagcttca gccacaccct ggtgtccttg agcctcttcg gctgtacaaa cattttctat
     1921 gaggaggaga acccaggggg ctgtgaagat gagtacctcg tcaaccccac ctgccaggtg
     1981 ctggttaagg atttcacctt cgagggcttc agccgcctcc gcttcctcaa cttgggccgc
     2041 atgattgatt gggtccctgt ggagtccctg ctgcggccgc ttaactccct ggctgccttg
     2101 gacctctcag gcattcagac gagcgacgcc gccttcctca cccagtggaa agacagcctg
     2161 gtgtccctcg tcctctacaa catggacctg tccgacgacc acatccgggt catcgtgcag
     2221 ctgcacaagc tgcgacacct ggacatctcc cgagaccgcc tctccagcta ctacaagttc
     2281 aagctgactc gggaggtgct gagcctcttt gtgcagaagc tggggaacct aatgtccctg
     2341 gacatctctg gccacatgat cctagagaac tgcagcatct ccaagatgga agaggaagcg
     2401 gggcagacca gcattgagcc ttccaagagc agcatcatac ctttccgggc tctgaagagg
     2461 ccgctgcagt tcctcgggct ctttgagaac tctctgtgcc gcctcacgca cattccagcc
     2521 tacaaagtaa gtggtgacaa aaacgaagag caggtgctga atgccatcga ggcctacacg
     2581 gagcaccggc ctgagatcac ctcgcgggcc atcaacttgc tttttgacat cgcccgcatc
     2641 gagcgttgca accagctgct gcgggccctg aagctggtca tcacggccct caagtgccac
     2701 aaatatgaca ggaacattca agtgacaggc agcgccgctc tcttctacct aacaaattcc
     2761 gagtaccgct cagagcagag tgtgaagctg cgccggcagg ttatccaggt ggtgctgaat
     2821 ggcatggaat cctaccagga ggtgacggtg cagcggaact gctgcctgac gctctgcaac
     2881 ttcagcatcc ccgaggagct ggaattccag taccgccggg tcaacgagct cctgctcagc
     2941 atcctcaacc ccacgcggca ggacgagtct atccagcgga tcgccgtgca cctgtgcaat
     3001 gccctggtct gccaggtaga caacgaccac aaggaggccg tgggcaagat gggctttgtc
     3061 gtgaccatgc tgaagctgat tcagaagaag ctgctggaca agacatgtga ccaggtcatg
     3121 gagttctcct ggagtgccct gtggaacatc acagatgaaa ctcctgacaa ctgcgagatg
     3181 ttcctcaatt tcaacggcat gaagctcttc ctggactgcc tgaaggaatt cccagagaag
     3241 caggaactgc ataggaatat gctaggactt ttggggaatg tggcagaagt gaaggagctg
     3301 aggcctcaac taatgacttc ccagttcatc agcgtcttca gcaacctgtt ggagagcaag
     3361 gccgatggga tcgaggtttc ctacaatgcc tgcggcgtcc tctcccacat catgtttgat
     3421 ggacccgagg cctggggcgt ctgtgagccc cagcgtgagg aggtggagga acgcatgtgg
     3481 gctgccatcc agagctggga cataaactct cggagaaaca tcaattacag gtcatttgaa
     3541 ccaattctcc gcctccttcc ccagggaatc tctcctgtca gccagcactg ggcaacctgg
     3601 gccctgtata acctcgtgtc tgtctacccg gacaagtact gccctctgct gatcaaagaa
     3661 ggggggatgc cccttctgag ggacataatt aagatggcga ccgcacggca ggagaccaag
     3721 gaaatggccc gcaaggtgat tgagcactgc agtaacttta aagaggagaa catggacacg
     3781 tctagatacc caactttctt gtacaaagtg gttgatctag agggcccgcg gttcgaactc
     3841 gagtcggccg ctggaggata cccctacgac gtgcccgact acgcctagaa ttctaccggg
     3901 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     3961 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     4021 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     4081 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     4141 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     4201 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     4261 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     4321 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     4381 catctccggg cctttcgacc tgcagcccaa gcttaccatg attgaacaag atggattgca
     4441 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     4501 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     4561 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     4621 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     4681 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     4741 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     4801 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     4861 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     4921 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     4981 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     5041 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     5101 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     5161 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gacgcccgac
     5221 cgaaaggagc gcacgacccc atgcatcgat aaaataaaag attttattta gtctccagaa
     5281 aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt
     5341 ttgcaaggca tggaaaatac ataactgaga atagagaagt tcagatcaag gttaggaaca
     5401 gagagacagc agaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     5461 cagggccaag aacagatggt ccccagatgc ggtcccgccc tcagcagttt ctagagaacc
     5521 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
     5581 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
     5641 gcccacaacc cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg
     5701 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     5761 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatgggta acagtttctt
     5821 gaagttggag aacaacattc tgagggtagg agtcgaatat taagtaatcc tgactcaatt
     5881 agccactgtt ttgaatccac atactccaat actcctgaaa tagttcatta tggacagcgc
     5941 agaaagagct ggggagaatt gtgaaattgt tatccgctca caattccaca caacatacga
     6001 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6061 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6121 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6181 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6241 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6301 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6361 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6421 ctataaagat accaggcgtt tccccttgga agctccctcg tgcgctctcc tgttccgacc
     6481 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6541 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6601 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6661 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6721 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6781 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6841 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6901 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6961 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7021 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7081 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7141 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7201 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7261 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7321 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7381 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7441 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7501 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7561 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7621 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7681 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7741 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7801 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7861 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7921 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7981 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8041 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     8101 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     8161 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     8221 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     8281 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     8341 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     8401 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     8461 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     8521 gggttttccc agtcacgacg ttgtaaaacg acggcgcaag gaatggtgca tgcaaggaga
     8581 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
     8641 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
     8701 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggcgat
     8761 tagtccaatt tgttaaagac aggatatcag tggtccaggc tctagttttg actcaacaat
     8821 atcaccagct gaagcctata gagtacgagc catagataaa ataaaagatt ttatttagtc
     8881 tccagaaaaa gggggg
//
