LOCUS       sequence-377567-       10210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prgef-1-Chrimson::UrSL2::wCherry unc-54 3' UTR C.elegans panneural 
            expression of Chrimson RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Lu Y, Ahamed T, Mulcahy B, Meng J, Witvliet D, Guan SA, Holmyard D, 
            Hung W, Wen Q, Chisholm AD, Samuel ADT, Zhen M
  TITLE     Extrasynaptic signaling enables an asymmetric juvenile motor circuit
            to produce symmetric undulation.
  JOURNAL   Curr Biol. 2022 Nov 7;32(21):4631-4644.e5. doi: 
            10.1016/j.cub.2022.09.002. Epub 2022 Sep 30.
  PUBMED    36182701
REFERENCE   2  (bases 1 to 10210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.09.002"; journalName: "Curr Biol"; date: 
            "2022-11-7- 7"; volume: "32"; issue: "21"; pages: "4631-4644.e5"
COMMENT     Sequence Label: pJH3628
FEATURES             Location/Qualifiers
     source          1..10210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    4300..4311
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             4957..5664
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(5099..5117)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5367..5386
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(7338..7355)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7338..7354)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7347..7369)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7567..8022
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7654..7673)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7864..7885
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8039..8058)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8158..8180
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8218..8236)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8304..8408
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8409..8477,8478..9269)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8627..8646)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9440..10028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9929..9948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10182..10199
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attcgaatgg
      241 ccatgggacg tcgacgcttg catgcctgca gcgtttccga taccccctta tatcagcaca
      301 cattcagtca tcataccaca aaatgttgtg atagtgatag gcaaaactac cgtatttcct
      361 ctattaatct tgcatgcaag actaattttc gattaacccg taggggtgca agactaatag
      421 agactgcaag actattagag gctgaaatac taattttcgt atgctcaata attttggaaa
      481 ttggcctatt ttttgtagaa acttgatacc gtttaaacaa ggaaaaatac acacttttta
      541 atatttcatc aataatttga acgattttgt gattttaagt tcaatttgcc caaaaaaaga
      601 caatttttct gacgttaccg caaagtaatg ctggtcaggc aaaaagagcg gtgcaaaaat
      661 ataagagact gcaatactaa tagaggaaat acggtaattg aattttagtg aaacttgcga
      721 cagttttcct catttttttg ttattccggc atacgagtgt ggcatacgag tgaggtcatc
      781 ttttgtttct tccgtttctt catcgctttt gaaaaaaaat gttgaagaaa acttcgtagc
      841 cgatagccgg ataatgtttg gcacggcgtt ccaacatata tcattggaaa ttttttaaga
      901 tttttgcgaa aaatcacatt cttcacgatg agaacacgtt attgaaggaa tataaatcaa
      961 gaataacata tagttatatt tctctattac tttaacgtta aatatgagca aatttgagca
     1021 ttttgattgc gatgaaaagc agaatcgagt caactgaaat ccgttcaaaa acctaagctc
     1081 ggttcgcccg aaagtcgatt tttccaatag ccgaacagca ctgcttcttt attttccagg
     1141 tatttttggg gtgtaagaat tatatcttct ttgtttgggt atttacgttt ccgaattccc
     1201 tttcttgatc tgtcgctatt tttcagaaac cagaacagtt tatccttttt catgagaacc
     1261 tgaatagctc aaaaacccgt ttaatttctt ttcatcatct catttgaaac tttcctagtc
     1321 ttcttaatga tttccaggct cctcctacat ttgtatctca agatgcatac actttatttc
     1381 cgctccaatt cgaatcatct acttctcttt tttcttcaac tttctacttt tttccattct
     1441 tttctttcaa atgttcgacg tcttcaagtg actgctcttc attctcctca tttttctcga
     1501 atttcattct tgtctctttt ttgctcaaaa atggaaaatg aaagtgacgt gagatttgga
     1561 cggcgggaca cgggggcagt agaagcagca aaaaggagag aaagaggaca caaataagaa
     1621 gaacgaattc aaaaataagc ggagaggagc tatttccgtc aattctacct ccccaatctt
     1681 catcaattcg gctcaattga atgacgtcac agggagataa accgtttgga tgagcgccgt
     1741 ccatcactga cgccatcccg tttgggacaa gaaaagagaa aaaagagcac aaagtttttg
     1801 gtgacggatc ttgtcaatca tatgaaagtt gttctgattg attgtcagtt tttttcctac
     1861 ttttttggat tctatccact tctgaacttt tgacaagttt caaactttct gaatcatttt
     1921 ctatgcattt tcctggaatt ctttttatgt aaaatatgaa atagaatgtt tttgaattca
     1981 agttctgctt ttttcttctt ttctgttctg ttcgggcttg ggtatgcttt ttttaaaaat
     2041 tattttgcac atcgaccaat aagtgcgcaa cttataaaat taatttattt ttgttaattt
     2101 ttgaaatact tgtattgctt taagtgatct gacctcgccc tgagctttcc acgtagttat
     2161 caaatacaaa tcctccaagg gtaacgtacc tatattactg atctttataa taactttatc
     2221 acctgtccag ttccagagga attctgttaa gcttataggc acagaaggag tcatttctgc
     2281 tggtttttaa tgatccaaat ctttatttca agtaaaaaac tgaacacttg cgaataaaac
     2341 tatcagatta accattcacc aaaaatgtgt ttgaatctaa aactttctca gtattccaaa
     2401 tatagaaata aataaccacg acattgctaa aatctgtctg aattgtgtac tccttaccgt
     2461 gaagtaataa tggatataat gaatcgtttg aaatgaatga tcagcacatt tttggtgaaa
     2521 gatcacaaat aaggaataag cgtcggaaaa taaaacgatt atttcggatc aaaaatttgt
     2581 tgaagatcat atacactcga gaccaagatt attctagaca attttcaaat tggcttcttt
     2641 gtttgcaaat cttcataata atctgtgaag tttggaaatt tgaattttta atcttttttc
     2701 atagattata gtttttattt ctttgcaaaa ctatattaaa aaccgatgca ttgttttagg
     2761 gaaattaatg agccttttgt tcaacactaa aaacaataaa attaaaattt tggcttcatc
     2821 atttgacctt ttttaagttc gaaaactttt ctcgtatttc tgaaccgcca attttttcac
     2881 acatctctag acttttggtg cccgttccag aaagttaagt aattgctatt ctaagaaagt
     2941 tctcaacatt gttttttagt tctgattgaa ttctgatgtt ccaggaatat attttaaatt
     3001 taatattctt tgcactattt ctatactaac taaataataa atagtctaag tatgttcaat
     3061 gagaacaaaa aagctctatc tatatttttc tatcctattt catttatatc cttttcattt
     3121 tgaactcacc agcttctctt cttcttcttg tccatatact tcttaaaagt ctcgaacttc
     3181 tctctctcac tctccatcaa tttctttatg gctaccgctt gcgctctgcc gcttccgaag
     3241 aaagaaggcg ttggcagagc tctcaattct attttttctc gccgtaggct atcgatttct
     3301 cagcttctct tctctcttcc tcactccacc accaccactt cgggcttctt cttttcttca
     3361 ttttcgtctt cttcttcatc ctcttctttt tttttcagtt ccccctcact cctcccctta
     3421 tatgcgcgtg cgagaggggt gcaaaagcag cgcgcatccg agaattgaaa cgagaaaacg
     3481 gagacgcagc agcagttcgt cctcagaaaa atagccagta aaaagagaaa aagatagaga
     3541 gagacctatc gcattttatt ttcaaattgc aattcctgca attcatgtgt gcgtgtgccc
     3601 attttcaatt cttcccgtta tttttcagac cattaccaac gttctttcta gatcttgaga
     3661 caatcttcct tctgctcaat cgttcgtcgt gaagacggca tcgacgacga cgacgatcag
     3721 cagtaaagga tccctatggc tgagctcatc tcctccgcta cccgttccct cttcgctgct
     3781 ggaggaatca acccatggcc gaacccatac caccacgagg acatgggatg cggaggaatg
     3841 accccaaccg gagagtgctt ctccaccgag tggtggtgcg acccatccta cggactctcc
     3901 gacgctggat acggatactg cttcgtcgag gctaccggag gtaagtttaa acttggactt
     3961 actaactaac catacatatt taaattttca ggatacctcg tcgtcggagt cgagaagaag
     4021 caggcttggc tccactcccg tggaacccca ggagagaaga tcggagctca ggtctgccag
     4081 tggatcgctt tctccatcgc tatcgctctc ctcaccttct acggattctc cgcttggaag
     4141 gctacctgcg gatgggagga ggtctacgtc tgctgcgtcg aggtcctctt cgtcaccctc
     4201 gagatcttca aggagttctc ctccccagct accgtctacc tctccaccgg aaaccacgct
     4261 tactgcctcc gttacttcga gtggctcctc tcctgcccag tcatcctcat ccgtctctcc
     4321 aacctctccg gactcaaggt aagtttaaac agttcggtac taactaaccc tgattattta
     4381 aattttcaga acgactactc caagcgtacc atgggactca tcgtctcctg cgtcggaatg
     4441 atcgtcttcg gaatggctgc tggactcgct accgactggc tcaagtggct cctctacatc
     4501 gtctcctgca tctacggagg atacatgtac ttccaggctg ctaagtgcta cgtcgaggct
     4561 aaccactccg tcccaaaggg acactgccgt atggtcgtca agctcatggc ttacgcttac
     4621 ttcgcttcct ggggttccta cccaatcctc tgggctgtcg gaccaggtaa gtttaaacat
     4681 atatatacta actaacggat tatatttaaa ttttcagagg gactcctcaa gctctcccca
     4741 tacgctaact ccatcggaca ctccatctgc gacatcatcg ctaaggagtt ctggaccttc
     4801 ctcgctcacc acctccgtat caagatccac gagcacatcc tcatccacgg agacatccgt
     4861 aagaccacca agatggagat cggaggagag gaggtcgagg tcgaggagtt cgtcgaggag
     4921 gaggacgagg acaccgtcgc ggtacccgcg gccgccgtga gcaagggcga ggaggataac
     4981 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     5041 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     5101 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     5161 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     5221 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     5281 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     5341 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     5401 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     5461 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     5521 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     5581 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     5641 ggcatggacg agctgtacaa gtaataggaa ttccaactga gcgccggtcg ctaccattac
     5701 caacttgtct ggtgtcaaaa ataatagggg ccgctgtcat cagagtaagt ttaaactgag
     5761 ttctactaac taacgagtaa tatttaaatt ttcagcatct cgcgcccgtg cctctgactt
     5821 ctaagtccaa ttactcttca acatccctac atgctctttc tccctgtgct cccaccccct
     5881 atttttgtta ttatcaaaaa acttctctta atttctttgt tttttagctt cttttaagtc
     5941 acctctaaca atgaaattgt gtagattcaa aaatagaatt aattcgtaat aaaaagtcga
     6001 aaaaaattgt gctccctccc cccattaata ataattctat cccaaaatct acacaatgtt
     6061 ctgtgtacac ttcttatgtt ttttacttct gataaatttt tttgaaacat catagaaaaa
     6121 accgcacaca aaatacctta tcatatgtta cgtttcagtt tatgaccgca atttttattt
     6181 cttcgcacgt ctgggcctct catgacgtca aatcatgctc atcgtgaaaa agttttggag
     6241 tatttttgga atttttcaat caagtgaaag tttatgaaat taattttcct gcttttgctt
     6301 tttggggttt cccctattgt ttgtcaagat ttcgaggacg gcgtttttct tgctaaaatc
     6361 acaagtattg atgagcacga tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag
     6421 tggaaggtga gtagaagttg ataatttgaa agtggagtag tgtctatggg gtttttgcct
     6481 taaatgacag aatacattcc caatatacca aacataactg tttcctacta gtcggccgta
     6541 cgggcccggt accatggtat tgatatctga gctccgcatc ggccgctgtc atcagatcgc
     6601 catctcgcgc ccgtgcctct gacttctaag tccaattact cttcaacatc cctacatgct
     6661 ctttctccct gtgctcccac cccctatttt tgttattatc aaaaaacttc tcttaatttc
     6721 tttgtttttt agcttctttt aagtcacctc taacaatgaa attgtgtaga ttcaaaaata
     6781 gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat taataataat
     6841 tctatcccaa aatctacaca atgttctgtg tacacttctt atgctcatag aaaaaaccgc
     6901 acacaaaata ccttatcata tgttacgttt cagtttatga ccgcaatttt tatttcttcg
     6961 cacgtctggg cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt
     7021 ttggaatttt tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt tgctttttgg
     7081 ggtttcccct attgtttgtc aagatttcga ggacggcgtt tttcttgcta aaatcacaag
     7141 tattgatgag cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa
     7201 ggtgagtaga agttgataat ttgaaagtgg agtagtgtct atggggtttt tgccttaaat
     7261 gacagaatac attcccaata taccaaacat aactgtttct actagtggcc acgtgggccg
     7321 tgcaccttaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     7381 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     7441 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     7501 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
     7561 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7621 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7681 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7741 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     7801 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7861 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg
     7921 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7981 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
     8041 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     8101 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     8161 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     8221 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     8281 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8341 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8401 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8461 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8521 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     8581 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     8641 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     8701 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     8761 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     8821 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     8881 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     8941 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9001 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9061 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9121 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9181 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9241 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9301 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9361 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9421 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9481 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     9541 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     9601 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     9661 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     9721 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     9781 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     9841 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     9901 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     9961 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10021 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10081 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    10141 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    10201 aagcggaaga
//
