LOCUS       sequence-377568-        7626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mpx probe generation for in situ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Biggs CM, Cordeiro-Santanach A, Prykhozhij SV, Deveau AP, Lin Y, Del
            Bel KL, Orben F, Ragotte RJ, Saferali A, Mostafavi S, Dinh L, Dai D,
            Weinacht KG, Dobbs K, Ott de Bruin L, Sharma M, Tsai K, Priatel JJ, 
            Schreiber RA, Rozmus J, Hosking MC, Shopsowitz KE, McKinnon ML, 
            Vercauteren S, Seear M, Notarangelo LD, Lynn FC, Berman JN, Turvey 
            SE
  TITLE     Human JAK1 gain of function causes dysregulated myelopoeisis and 
            severe allergic inflammation.
  JOURNAL   JCI Insight. 2022 Dec 22;7(24):e150849. doi: 
            10.1172/jci.insight.150849.
  PUBMED    36546480
REFERENCE   2  (bases 1 to 7626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "JCI 
            Insight."; date: "2022-12-22"; volume: "7(24):e150849. doi"; pages: 
            " 10.1172/jci.insight.150849"
COMMENT     Sequence Label: pBK-CMV-mpx
FEATURES             Location/Qualifiers
     source          1..7626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     promoter        130..487
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        130..321
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(153..173)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      338..473
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             522..1316
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             522..1316
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(576..595)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1186..1205
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1504..1523)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1548..1595
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1924..2512
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2413..2432
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2687..2990
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2991..3194
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3145..3165
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3303..3319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3308..3330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3327..3343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3327..3343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3364..3382
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6636..6655)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6637..6655)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6662..6679)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6662..6678)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6671..6693)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    7040..7161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7077..7096)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7131..7150
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7168..7623)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7305..7326)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7517..7536
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
      181 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
      241 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      301 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
      361 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
      421 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
      481 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
      541 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
      601 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
      661 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
      721 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
      781 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
      841 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
      901 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
      961 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     1021 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     1081 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     1141 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     1201 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     1261 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     1321 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     1381 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     1441 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
     1501 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     1561 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     1621 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
     1681 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     1741 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
     1801 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     1861 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1921 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1981 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2041 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     2101 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2161 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2221 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2281 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2341 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2401 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2461 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2521 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2581 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt
     2641 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     2701 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     2761 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     2821 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     2881 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     2941 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     3001 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     3061 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     3121 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     3181 atataagcag agctggttta gtgaaccgtc agatccgcta gccgcaatta ctgtgagtta
     3241 gctcactcat taggcacccc aggctttaca ctttatactt ccggctcgta tattgtgtgg
     3301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccttgatt acgccaagct
     3361 cgaaattaac cctcactaaa gggaacaaaa gctggagctc gcgcgcctgc aggtcgacac
     3421 tagtggatcc aaagaattcg gcacgaggct ctttcagtgg gggcagaaga agaaagtcct
     3481 ggaagacctt ttattcttga ttcaattgag gaagcaaaga aaatagtgga cgctgcttac
     3541 aagtattctc gagatgagag tttagcaagg gtgcgcaaag atgtcattaa gccttctgac
     3601 aagctacgtc tgctgaaaca gccagctcga aagacacggg aggccgtaag agctgcagac
     3661 tacatggcac aaacgctgag actgatcagt gagaaagccc atcatgtaca caagaggtcc
     3721 atcaatgcca caaacctgct cactcaagac gagcttcaaa ccattacacg tatgacaggc
     3781 tgtgaagctc aaacccggcc accatcttgt aggaccacac ccctcataaa caagtatcga
     3841 actgccagcg gtgtctgcaa caatcggaag aacccacttc ttggtgcatc caacactcca
     3901 tttgcccgct ggttgcctgc tcaatatgag gacgccgttt ctcagccaaa aggatgggat
     3961 cccaacaagt tgtacaatgg tgcagcgctg cccatggtta gactggtttc caatcgcatt
     4021 ctagccactg cagatgctga catagagagt gaccatgact ttaccttcat gctcaccatc
     4081 tttgggcaat gggttgacca cgatctgact tttacacctt tctcaccaag tattaggtcc
     4141 tttagtaatg gcattgactg tgaaaacagt tgtgagcgct ctgaaccctg cttcccaatt
     4201 tcggcccctc caggagatca gcgtctgcgc cctaacacct gtctccctgt cttccggtca
     4261 gcaccgactt gtggttctgg gcacactgcc tacatgtttg gtgaagtccc aaatgttcgg
     4321 gaacagatca atactctaac agcttaccta gatgctggac aagtttacgg ttcagaggat
     4381 ggactagcaa aggaactgcg ggaccttact aatgatggtg gtcttttacg tgtcaataat
     4441 cgttttaaag acaatggacg agagctcctg ccctttacta gtgttaacac caacttgtgc
     4501 gccactcgtc agaaaatcct caacgacagc actctgacag aggttccttg cttcattgcg
     4561 ggtgatgctc gtgtcgatga gaatcctgct ctgaactcct tacacacact ttttgtaagg
     4621 gagcacaata gattggctcg tgcccttcat gtgctcaacc ctacatggag tagtgaaact
     4681 ctttatcaag aagcaagaaa aattgttggt gcattcaacc agatcttggt gatcaaggaa
     4741 tacttgccac taattgtagg ccctgatgct tacaatagac accttggacc atatccaggt
     4801 tacaatgaga atgtggaccc taccattgcg aacgtctttg ccactgctgc tttccgtttt
     4861 gcccacttga ctatccagcc cttcattttt cgtcttgatg aaaactacaa gaaccatcct
     4921 cagtttccca gtgtccccct ctatgaggct ttcttctctc catggagggt gatctttgaa
     4981 gggggaattg atcctgtgct ccgtggattg attggtcgtc cggcaaaact gaacacccag
     5041 gaccacatgt tggtgaatgc tctcagagag aggctgtttg ccttcacatc ccacatagct
     5101 ttggatctgg cttccctcaa catgcaaaga gggcgtgacc atgctatacc aggttataat
     5161 gcatggcgtc ggttctgtgg attgtctgct cctcagaatg agcaagaatt ggctgtggtg
     5221 atgaacaaca ctaaactagc ccgcaagtta attgagcttt atggcacccc tgagaacatt
     5281 gatgtttggt taggaggtgt tgctgagcct tttgctcctg gtggtcgtgt cggttctctt
     5341 tttgcctgcc tgatttcaag acagttccag aaaatccgag atggcgatag gttgtggttt
     5401 gagagcaatg gagtgttcac caccaaacag aaaacagctc tggcttctgt gtcaatggcc
     5461 cgcataatct gcgacaacac cggaatcctt aaagttccca gagatccttt tcgcttccgg
     5521 agtcctgcca gttttgttaa ctgtgggaat atcccagctt ttgatctcga gccttggaaa
     5581 gagactggtg acggtggcat caacatttca gacattggaa atgagataga gattccatcc
     5641 caggacctcc cggacccagg ggacctccag gagaacgtgg tccacagggt gtagcagggc
     5701 caccaggacc tccaggaatt ccaggacctc ccattaatac aacaggacaa cagtctgcct
     5761 tctttgcctc agtcaactcc atccttcctg ccactgctaa agttgtggtg ttcggtcagg
     5821 tcctttacaa tggacaaaac cattataacc aaacctcagg gatgttcttg tgccagatac
     5881 ctggtgtata tgaattcgag ttttcatgca tagggactcg tagtttgggc tttgtgacct
     5941 taaagaaaaa taatagggta gagcttactc ctgaaacagt tgctcttaat acaaggtcat
     6001 tagctgaagg gaaggcagtc ctgagcctac agagaggaga ccgtgtgtat gtggaggttt
     6061 ctcgaagtgc taatggtata ggcttttcaa gctatttctc tggtcacatt ctttttcccg
     6121 tttgaggcta ttacttataa tgcattacta tttccctttt ttttttgttc attttaaatg
     6181 cattctgctt ctttctattt gtctttaaac atgtttcttc ttactgtaat gtcttaggct
     6241 ctgttgttgt tgttgttgtt gttttattaa taattagctt tgcaaaatta gtagtatggt
     6301 tagagaatgc cttatgtttt aattcagaat tttttacagg taaataagtg ataaaatttc
     6361 agaaccaaga tttaactgct aatgcatcga catttctttt atgaagtttt ttttttattt
     6421 gttgtttttg gttttgttgt cagattgcaa caaaattttc ggtagtccat gctgtgacag
     6481 ttatttttgt gtatgctttt agcaaagtaa aaaaactaat aaataaagtt ttttgtcnaa
     6541 aaaaaaaaaa aaaaaactcg agagtacttc tagagcggcc gcgggcccat cgattttcca
     6601 cccgggtggg gtaccaggta agtgtaccca attcgcccta tagtgagtcg tattacaatt
     6661 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     6721 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     6781 gcccttccca acagttgcgc agcctgaatg gcgaatggag atccaatttt taagtgtata
     6841 atgtgttaaa ctactgattc taattgtttg tgtattttag attcacagtc ccaaggctca
     6901 tttcaggccc ctcagtcctc acagtctgtt catgatcata atcagccata ccacatttgt
     6961 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     7021 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     7081 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7141 caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     7201 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     7261 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     7321 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     7381 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     7441 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     7501 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     7561 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     7621 cgtcag
//
