LOCUS       sequence-377573-        7414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pacr-2s-Chrimson::wCherry unc-54 3' UTR C.elegans A/B MN expression 
            of Chrimson RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Lu Y, Ahamed T, Mulcahy B, Meng J, Witvliet D, Guan SA, Holmyard D, 
            Hung W, Wen Q, Chisholm AD, Samuel ADT, Zhen M
  TITLE     Extrasynaptic signaling enables an asymmetric juvenile motor circuit
            to produce symmetric undulation.
  JOURNAL   Curr Biol. 2022 Nov 7;32(21):4631-4644.e5. doi: 
            10.1016/j.cub.2022.09.002. Epub 2022 Sep 30.
  PUBMED    36182701
REFERENCE   2  (bases 1 to 7414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.09.002"; journalName: "Curr Biol"; date: 
            "2022-11-7- 7"; volume: "32"; issue: "21"; pages: "4631-4644.e5"
COMMENT     Sequence Label: pJH3650
FEATURES             Location/Qualifiers
     source          1..7414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2571..2582
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             3228..3935
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3370..3388)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3638..3657
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4854..4876)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4976..4995
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5073..5091)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5159..5263
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5264..5332,5333..6124)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5482..5501)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6295..6883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6784..6803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7037..7054
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7171..7192
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7207..7212,7213..7230,7231..7237)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7245..7261
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7250..7272
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7269..7285
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7269..7285
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagcatgc ggccgctcac caccgactga ttttcatttc aaaattaatg
       61 cagatttcta cgtgttcttt ttcaaataaa tttatatatt ttttgttctc tcacaaaata
      121 aataatttcg agagttaagt gatcattctc agacatattc tataattgtc cgtcaggaaa
      181 cagatagagg aacctttgat aaataaatta taaaaggccg acagggaaat gcttctccct
      241 cgactctgtt agaagcgaag aggcaatttt taaaaatttc ccatttttat gcgtttttct
      301 gatttccaaa gtcacgcatc atttaccaac cgcaacgatt cataaggacg taattttgat
      361 tttctggacg atttttatat agttttgatg tgaaattcgc tttatttgtc tgaaagtgga
      421 atagatttag tgtggattta accgaaaaat gcagatcttt taaaaattta aaccagtgtt
      481 ttgttgcctt atttgcgaaa aaaaattgtg atatttcgag tattgctgca atttgattag
      541 taaaatgtta ggatataagt atttaaaaag tataattggt tgaattttct ttttcatcac
      601 cgacttccaa aaatatagag atactgagtt ttcaccattt tttgaatgta aataaaaaca
      661 ttttgaacac ttgtgctggg attcaatcca aatacattca ctgtcattta atattattat
      721 atatcaaaat aaaacgcata actttttctg ttttttttta aattttttca aaatgtaggt
      781 ttggtaagtt aaggtaaggt taggcaatgt aggtaaacac gagatgatac tccacgttga
      841 aaattgtttt cataaataca gtttagattg aactgaagga ttttatcgtc taatagaata
      901 taatttttta gttcaagatt taggatttgt tttcactttt ttttagattc aagacatata
      961 tctcgcaaaa tgagttttgt ctttgagcca tcttgtcatc atagcacatt gaattttgtt
     1021 gttcgcaaga cggaatatat gttcccagct gcaaattttg tgtcggatat ttcttcttta
     1081 gcgaaaggcg ttaaaaacca aaaatagtcg aagcgtttga tatgtgtccc gttggttttt
     1141 cgtgtccatc tattctgttt cgagtacaca tgttgcgacg cggcactgat gaaaaagtga
     1201 aagagctagt gtcacacaca aacacaaagt atgctcatgt gagtgtgtag atatgtgtag
     1261 atgtatgtcc tgaaggaata aaaagacttt ttgaagctac atttttaaac aaaaaaagac
     1321 agaaacatga agcacaactc tctattctgt cgaacggcgt gtgcaactca cgcacaccca
     1381 aaaatatgaa atataacatt cgcaccaaca aagcgcgaat atcaatgaat ctcgagggaa
     1441 cgatggctgc ctgccggcgc gtgttgctct tctgtcattg ggataactgg cgggtctgtt
     1501 gggtccgtcc tttagaccat ttgactacac tgccctgcat atgtgtgaaa cgtcgtcgtt
     1561 gctgttggga gctgtctgtg tgtatgtgtg tgtgtgtgag tatggagcag taaaaagctg
     1621 ccgatacgag aagcgccgcc ccggacggac ggcgatggcg catcgccaaa tgtgtcattt
     1681 tagttgtctt cacgaaccag agggcccacg acggacgggc ggccacgccc acctcaacca
     1741 gaatatcctt ccttctccca cccacccttg cggacgcgcg gatgcgctga acaaaaagac
     1801 gacgaagaag aagaagaaga agctgaacga agaaaaaagc atcaaaagaa gtgaagcggc
     1861 tctaccaccc cgaccacctt ccttcacagg aaggacgccg ttttcatccc gggattggcc
     1921 aaaggaccca aaggtatgtt tcgaatgata ctaacataac atagaacatt ttcaggagga
     1981 cccttggcta gcgtcgacgg atccctatgg ctgagctcat ctcctccgct acccgttccc
     2041 tcttcgctgc tggaggaatc aacccatggc cgaacccata ccaccacgag gacatgggat
     2101 gcggaggaat gaccccaacc ggagagtgct tctccaccga gtggtggtgc gacccatcct
     2161 acggactctc cgacgctgga tacggatact gcttcgtcga ggctaccgga ggtaagttta
     2221 aacttggact tactaactaa ccatacatat ttaaattttc aggatacctc gtcgtcggag
     2281 tcgagaagaa gcaggcttgg ctccactccc gtggaacccc aggagagaag atcggagctc
     2341 aggtctgcca gtggatcgct ttctccatcg ctatcgctct cctcaccttc tacggattct
     2401 ccgcttggaa ggctacctgc ggatgggagg aggtctacgt ctgctgcgtc gaggtcctct
     2461 tcgtcaccct cgagatcttc aaggagttct cctccccagc taccgtctac ctctccaccg
     2521 gaaaccacgc ttactgcctc cgttacttcg agtggctcct ctcctgccca gtcatcctca
     2581 tccgtctctc caacctctcc ggactcaagg taagtttaaa cagttcggta ctaactaacc
     2641 ctgattattt aaattttcag aacgactact ccaagcgtac catgggactc atcgtctcct
     2701 gcgtcggaat gatcgtcttc ggaatggctg ctggactcgc taccgactgg ctcaagtggc
     2761 tcctctacat cgtctcctgc atctacggag gatacatgta cttccaggct gctaagtgct
     2821 acgtcgaggc taaccactcc gtcccaaagg gacactgccg tatggtcgtc aagctcatgg
     2881 cttacgctta cttcgcttcc tggggttcct acccaatcct ctgggctgtc ggaccaggta
     2941 agtttaaaca tatatatact aactaacgga ttatatttaa attttcagag ggactcctca
     3001 agctctcccc atacgctaac tccatcggac actccatctg cgacatcatc gctaaggagt
     3061 tctggacctt cctcgctcac cacctccgta tcaagatcca cgagcacatc ctcatccacg
     3121 gagacatccg taagaccacc aagatggaga tcggaggaga ggaggtcgag gtcgaggagt
     3181 tcgtcgagga ggaggacgag gacaccgtcg cggtacccgc ggccgccgtg agcaagggcg
     3241 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     3301 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     3361 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     3421 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     3481 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     3541 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     3601 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     3661 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     3721 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     3781 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     3841 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     3901 actccaccgg cggcatggac gagctgtaca agtaatagga attccaactg agcgccggtc
     3961 gctaccatta ccaacttgtc tggtgtcaaa aataataggg gccgctgtca tcagagtaag
     4021 tttaaactga gttctactaa ctaacgagta atatttaaat tttcagcatc tcgcgcccgt
     4081 gcctctgact tctaagtcca attactcttc aacatcccta catgctcttt ctccctgtgc
     4141 tcccaccccc tatttttgtt attatcaaaa aacttctctt aatttctttg ttttttagct
     4201 tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa
     4261 taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc
     4321 tacacaatgt tctgtgtaca cttcttatgt tttttacttc tgataaattt ttttgaaaca
     4381 tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt ttatgaccgc
     4441 aatttttatt tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa
     4501 aagttttgga gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc
     4561 tgcttttgct ttttggggtt tcccctattg tttgtcaaga tttcgaggac ggcgtttttc
     4621 ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa gatcggaaga aggtttgggt
     4681 ttgaggctca gtggaaggtg agtagaagtt gataatttga aagtggagta gtgtctatgg
     4741 ggtttttgcc ttaaatgaca gaatacattc ccaatatacc aaacataact gtttcctact
     4801 agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     4861 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     4921 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     4981 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     5041 aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat ttttataggt
     5101 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     5161 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5221 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5281 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5341 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5401 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5461 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5521 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5581 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5641 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5701 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5761 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5821 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5881 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5941 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6001 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6061 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6121 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6181 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6241 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6301 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6361 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6421 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6481 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6541 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6601 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6661 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6721 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6781 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6841 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6901 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6961 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     7021 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     7081 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     7141 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     7201 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     7261 atttcacaca ggaaacagct atgaccatga ttacgccaag ctgtaagttt aaacatgatc
     7321 ttactaacta actattctca tttaaatttt cagagcttaa aaatggctga aatcactcac
     7381 aacgatggat acgctaacaa cttggaaatg aaat
//
