LOCUS       sequence-377600-        5637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cyanobacterial carboxysome reporter with mNeonGreen driven by native
            rbcS promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Sakkos JK, Hernandez-Ortiz S, Osteryoung KW, Ducat DC
  TITLE     Orthogonal Degron System for Controlled Protein Degradation in 
            Cyanobacteria.
  JOURNAL   ACS Synth Biol. 2021 Jul 16;10(7):1667-1681. doi: 
            10.1021/acssynbio.1c00035. Epub 2021 Jul 7.
  PUBMED    34232633
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00035"; journalName: "ACS Synth Biol"; date: 
            "2021-07-16- 16"; volume: "10"; issue: "7"; pages: "1667-1681"
COMMENT     Sequence Label: pAM2314-RbcS-mNG
FEATURES             Location/Qualifiers
     source          1..5637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      725..1313
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1214..1233
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1467..1484
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1499..1639
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1565..1584)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1725..1747
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2684..3343)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3258..3277
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3344..3445)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
       61 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
      121 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
      181 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
      241 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
      301 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
      361 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
      421 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
      481 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
      541 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
      601 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
      661 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
      721 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      781 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      841 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
      901 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      961 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1021 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1081 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1141 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1201 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1261 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1321 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1381 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     1441 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     1501 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     1561 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     1621 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     1681 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     1741 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag gatcgatccg gcagccggcg
     1801 gagcgctgct ttcttggcaa gcggtcgcca gccccaacgc cagggctgcc agcccgaaac
     1861 agcggggcaa ggcagcttgg aagggcgatc gcagcacggg catggcaatg tctctctgaa
     1921 ggaatgcaga ccttattcgt acagccaggg ttgaatcgtg ggggtccaat cacttagctc
     1981 tgctgggcta aaccagagag caatttcctg ttgtgctgtt tcgattgcat ccgagccatg
     2041 gatgatgttg cggccaatat tgacaccaaa atcaccacgg atggtgcccg gttctgccgt
     2101 cagcggattg gtagcgccga tcaacttgcg agcagccgcc acaacgcctt cgccttccaa
     2161 gacgatcgcc acgatcggcc cagaggtgat gaactcgacg aggccattga agaaggggcg
     2221 ctcgcggtgg acagcatagt gctgttcggc cagctcgcga ctgggcttca gctgctttag
     2281 gcccaccagt ttgaagcctt tttgctcaaa gcggccgatg atcgtaccga ccaaaccccg
     2341 ctgaacgcca tcgggcttga tggcaataaa tgtgcgttcc acagacatct agatagtcct
     2401 caagacgagg caagcattga gcttgccttc ctatggttcg ggatcactgg gattcttgac
     2461 aagcgatcgc ggtcacatcg ctatctctta ggacttcgca gcgggcgagt cggattgacc
     2521 cggtagggat ttcgccagat caatgcccgt ggtttgtttc agcttctcca gcaagctagc
     2581 gatttgggta gcgctgcctt ccccttcgcc aatcacagtg atcgactcca cgtcgatatc
     2641 tggcacggtg cctgaaagcg tgacgagcag ggactcgaag cttttacgcc ccgccctgcc
     2701 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga
     2761 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     2821 tgcccatagt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     2881 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag
     2941 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     3001 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     3061 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     3121 tacggaactc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     3181 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     3241 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     3301 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     3361 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     3421 agttggaacc tcttacgtgc cgatcggagc ctctgactat cgctggggga gtgagcgttg
     3481 ctgcgtaaag ctttctcccc agcctttcga cttaaccttt caggatttct gaatcatgag
     3541 catgaaaact ctgcccaaag agcgtcgttt cgagactttc tcgtacctgc ctcccctcag
     3601 cgatcgccaa atcgctgcac aaatcgagta catgatcgag caaggcttcc accccttgat
     3661 cgagttcaac gagcactcga atccggaaga gttctactgg acgatgtgga agctccccct
     3721 gtttgactgc aagagccctc agcaagtcct cgatgaagtg cgtgagtgcc gcagcgaata
     3781 cggtgattgc tacatccgtg tcgctggctt cgacaacatc aagcagtgcc aaaccgtgag
     3841 cttcatcgtt catcgtcccg gccgctacgg aagtggcagc ggtagtatgg tcagcaaagg
     3901 tgaagaagac aatatggcga gcctgccggc gacgcatgaa ctgcacattt tcggtagcat
     3961 caacggtgtg gacttcgata tggttggcca gggtaccggc aacccgaatg acggttatga
     4021 agaactgaat ctgaaaagta cgaagggcga tctgcaattt tccccgtgga ttctggtccc
     4081 gcatatcggt tacggcttcc accagtacct gccgtatccg gatggtatga gcccgtttca
     4141 agcggccatg gtggacggtt ctggctatca ggttcatcgt accatgcaat tcgaagatgg
     4201 cgcgagtctg accgtgaact atcgctacac gtatgaaggt tcacacatta aaggcgaagc
     4261 gcaggtcaaa ggtaccggtt ttccggcgga tggtccggtg atgaccaact cactgacggc
     4321 agctgattgg tgccgttcga aaaagaccta tccgaatgac aaaaccatta tcagcacgtt
     4381 taagtggtct tacaccacgg gtaacggcaa acgttatcgc agcaccgcac gtaccacgta
     4441 cacgttcgct aaaccgatgg cggccaacta cctgaaaaat cagccgatgt atgtttttcg
     4501 caaaaccgaa ctgaaacatt ccaagacgga actgaacttc aaggaatggc aaaaggcgtt
     4561 tacggatgtg atgggtatgg acgaactgta caagtgagga tccccgggta ccgagctcga
     4621 atttcgagct tctggagcag gaagatgtcg cgggcattag caccagcggt ctgccaagcc
     4681 tccgccagcc gttgggtccc ttccgcttga gcttttccat cttcgacgat acgggcggcg
     4741 gccccccgcg cttccgcgat cgcccgttta caagctgcct cagctggggc gatcacatcg
     4801 gcttgaagtt gctgctgcac ctgtttgatc cgctcctgct gcacagggag ttctgcttgg
     4861 ctacgagcga cttcggtagc aatgtccgct tcagcttcgg ccaccaccgc ttcgcgccgc
     4921 gtcaacgcat cctgaatccg gcgctcggcc tcggcttggg cgatcgctac atcgcgatcg
     4981 atccgacgca gggccgtgat cttgtcattt tcggccgttt ggatcgcaga ggcagcctgg
     5041 gcatcggctt cagcaattcg ggcatctcgc tgcagatcag cccgctgctt gcgtccacta
     5101 gccgagagat aaccgacctc atcggaaatg ttctggactt gcagcgtatc gaggactaga
     5161 cccagctgct caaggtcatc ctccgcctct tccagcagac ttttggcaaa ggcaattttg
     5221 tcctcgttga tctgctccgg cgtgaggctg gctaaaacac cacgcaagtt gccttcgagg
     5281 gtctccttgg caatttgctc gatttcctta cggtttttgc caagcagccg ctcgatcgcg
     5341 ttgtggatgg tcggttcttc cccagcaatc ttgatattgg caacgccttc aacagtcagg
     5401 ggaatgccgc ccttggagaa ggcattggaa acgcgcaact caatgatcat gttggtcaga
     5461 tccatgcgga gcgctttttc cagcagaggt acccgcaggc tgctgccgcc cttgaccaag
     5521 cgatagccaa ctcggcggcc atcactactg cggcgactac tgccagcaaa gatcaaaatt
     5581 tcactgggtt ggcagatgta gtagagattg cgcaggacta agctgccagc cccggcg
//
