LOCUS       sequence-37764-e         483 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 483)
  TITLE     mammalian homologous recombination reporter genes
REFERENCE   2  (bases 1 to 483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGCGFP-G418
FEATURES             Location/Qualifiers
     source          1..483
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     133..152
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     392..413
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      455..464
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc
       61 tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc
      121 acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg
      181 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
      241 ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc
      301 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc
      361 tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
      421 accaccccgg tgaacagctc ctcgcccttg ctcaccatgg tggcgaccgg tggatccacg
      481 ccg
//
