LOCUS       sequence-377699-        8339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for expression of diacylglycerol 
            acetyltransferase from Euonymus alatus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8339)
  AUTHORS   Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio
            S, Orzaez D, Patron N
  TITLE     Tunable control of insect pheromone biosynthesis in Nicotiana 
            benthamiana.
  JOURNAL   Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048.
  PUBMED    37032497
REFERENCE   2  (bases 1 to 8339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048."
COMMENT     Sequence Label: GB1022
FEATURES             Location/Qualifiers
     source          1..8339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..141
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     56..75
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(156..173)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(327..915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(407..426)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1003..1818)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1003..1818)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1726..1745
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    2251..2273
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      4030..4278
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             5605..6234
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6663..7736
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7802..7996
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8232..8254)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
       61 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
      121 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      181 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      241 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      301 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      361 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      421 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      481 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      541 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      601 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      661 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      721 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
      781 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      841 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      901 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      961 gaaaactcac gttaagggat tttggtcatg cattctaggt gattagaaaa actcatcgag
     1021 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
     1081 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
     1141 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
     1201 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
     1261 caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
     1321 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagtcgaaa
     1381 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
     1441 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
     1501 tgctgttttc cctgggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
     1561 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
     1621 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
     1681 cttcccatac aatcggtaga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
     1741 atacccatat aaatcagcat ccatgttgga atttaatcgc ggccttgagc aagacgtttc
     1801 ccgttgaata tggctcataa cagaacttat tatttccttc ctcttttcta cagtatttaa
     1861 agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct
     1921 aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca
     1981 tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc
     2041 gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt
     2101 tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct
     2161 cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag
     2221 ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg taacaagctt
     2281 cgtctcagtc aggaggtcga cgagtcagta ataaacggcg tcaaagtggt tgcagccggc
     2341 acacacgagt cgtgtttatc aactcaaagc acaaatactt ttcctcaacc taaaaataag
     2401 gcaattagcc aaaaacaact ttgcgtgtaa acaacgctca atacacgtgt cattttatta
     2461 ttagctattg cttcaccgcc ttagctttct cgtgacctag tcgtcctcgt cttttcttct
     2521 tcttcttcta taaaacaata cccaaagagc tcttcttctt cacaattcag atttcaattt
     2581 ctcaaaatct taaaaacttt ctctcaattc tctctaccgt gatcaaggta aatttctgtg
     2641 ttccttattc tctcaaaatc ttcgattttg ttttcgttcg atcccaattt cgtatatgtt
     2701 ctttggttta gattctgtta atcttagatc gaagacgatt ttctgggttt gatcgttaga
     2761 tatcatctta attctcgatt agggtttcat agatatcatc cgatttgttc aaataatttg
     2821 agttttgtcg aataattact cttcgatttg tgatttctat ctagatctgg tgttagtttc
     2881 tagtttgtgc gatcgaattt gtcgattaat ctgagttttt ctgattaaca gaatgatgga
     2941 tgcacatcag gaaataaaga acttcatcaa ggtgtgggtt caagcaatgg tttgcttatc
     3001 ttatgcttat tacttctcca gtaggctacc aaagggtttg cttcggcttc tttcactttt
     3061 gccagtgttg tacttacttt tgatcgcacc tttgaatatc agctcattca tcttatcaag
     3121 tataacggga ttttttctag cttggcttac cacctttaag gtcattagct ttgcattcga
     3181 tcaaggccca ttatacccat tacctcagaa tcttcttcat tttatctcaa ttgcttgtct
     3241 ccccataaca attaaaagaa atccaagccc aaaattgaaa tctacaacta atccatcacc
     3301 aatcagtcat cttcttaaaa aagcctttat gagttttcca tccaaggtgc tattccattg
     3361 ggttatcgct catctgtacc aatacaaaaa atatatggac ccgaacgttg tgctcgtgat
     3421 atattgttgt catgtttacg tgatgttgga tatcagtttg agtctttgcg ccactttagc
     3481 agagttcctt tgtggttttg atgttgatcc tcagttcaag gaaccatatt tagccacttc
     3541 tttgcaggac ttctggggac ggaggtggaa cattatagtc tcttcagtct tgaggtctac
     3601 tgtttatgca ccgacgcgta acatcgcttc atacctcata ggctccaggt gggcctactt
     3661 ccctgccatt atcgcaacgt tcgttgtctc tggggtgatg catgatgtcg tatattatgt
     3721 ttatatgatg cacatgtatc ccaagtggga tatgaccgga cattttgtat tgcacggtat
     3781 ttgtgaagcc ctggaagtgg agatgaaatg caaaagatct cgatcagata aatggaggag
     3841 acaccctgcc gtcgactggg tgatggttat ggggttcgtt atgggaacct ctgtgtcttt
     3901 gttatttgta cctcttctaa gagacaatgt tgaccaaata gtggctgaag aatattctat
     3961 tctttttaat ttcgttaggg aaaaaatcgt tatgttgggt acaagattcg tgtgcggcaa
     4021 ttgagcttat atgaagatga agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc
     4081 ttaagtttgt gtttttttct tggcttgttg tgttatgaat ttgtggcttt ttctaatatt
     4141 aaatgaatgt aagatctcat tataatgaat aaacaaatgt ttctataatc cattgtgaat
     4201 gttttgttgg atctcttctg cagcatataa ctactgtatg tgctatggta tggactatgg
     4261 aatatgatta aagataagcg cttgagacga agcttgacgt aaacctaaga gaaaagagcg
     4321 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
     4381 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
     4441 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcatctgaa aacgacatgt
     4501 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
     4561 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
     4621 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
     4681 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
     4741 agatcaccgg caccaggcgc gaccgcctgg agctggccag gatgcttgac cacctacgcc
     4801 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
     4861 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
     4921 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
     4981 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
     5041 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
     5101 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
     5161 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
     5221 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
     5281 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
     5341 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
     5401 gcacctctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
     5461 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
     5521 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
     5581 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
     5641 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
     5701 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
     5761 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
     5821 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
     5881 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
     5941 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
     6001 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
     6061 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
     6121 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
     6181 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
     6241 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
     6301 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
     6361 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
     6421 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
     6481 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
     6541 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
     6601 agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
     6661 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
     6721 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
     6781 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
     6841 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
     6901 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
     6961 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
     7021 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
     7081 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
     7141 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
     7201 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
     7261 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
     7321 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
     7381 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
     7441 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
     7501 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
     7561 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
     7621 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
     7681 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
     7741 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggactctttc
     7801 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
     7861 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
     7921 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
     7981 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
     8041 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
     8101 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
     8161 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
     8221 gacggtgaaa acctctgaca catgcagctc ccggtgacgg tcacagcttg tctgtaagcg
     8281 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcgggg
//
