LOCUS       sequence-377701-        9214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for expression of alcohol O-acetyltransferase 
            from Saccharomyces cerevisiae S288C, codon optimized for Nicotiana..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio
            S, Orzaez D, Patron N
  TITLE     Tunable control of insect pheromone biosynthesis in Nicotiana 
            benthamiana.
  JOURNAL   Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048.
  PUBMED    37032497
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048."
COMMENT     Sequence Label: GB3681
FEATURES             Location/Qualifiers
     source          1..9214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      848..1042
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1278..1300)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1386..1526
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1441..1460
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1541..1558)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1712..2300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1792..1811)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2388..3203)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2388..3203)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3111..3130
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    3636..3660
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4298..4642
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4586..4606
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    6540..6564
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7865..8494
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(8923..9214,1..782)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
       61 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
      121 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
      181 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
      241 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
      301 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
      361 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
      421 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga
      481 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
      541 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
      601 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
      661 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
      721 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
      781 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac
      841 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc
      901 cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata
      961 taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta
     1021 aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag
     1081 cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg
     1141 ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag
     1201 ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc
     1261 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg tgacggtcac agcttgtctg
     1321 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     1381 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     1441 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     1501 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     1561 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1621 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1681 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1741 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1801 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1861 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1921 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1981 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2041 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2101 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     2161 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2221 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2281 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2341 ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtgatta gaaaaactca
     2401 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga
     2461 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
     2521 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
     2581 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
     2641 aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
     2701 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgagt
     2761 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
     2821 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
     2881 tggaatgctg ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
     2941 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc
     3001 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
     3061 tcgggcttcc catacaatcg gtagattgtc gcacctgatt gcccgacatt atcgcgagcc
     3121 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct tgagcaagac
     3181 gtttcccgtt gaatatggct cataacagaa cttattattt ccttcctctt ttctacagta
     3241 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
     3301 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
     3361 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
     3421 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
     3481 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
     3541 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
     3601 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
     3661 ataacaagct tcgtctcagt caggagacta gagccaagct gatctccttt gccccggaga
     3721 tcaccatgga cgactttctc tatctctacg atctaggaag aaagttcgac ggagaaggtg
     3781 acgataccat gttcaccacc gataatgaga agattagcct cttcaatttc agaaagaatg
     3841 ctgacccaca gatggttaga gaggcctacg cggcaggtct gatcaagacg atctacccga
     3901 gtaataatct ccaggagatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat
     3961 tcaggactaa ctgcatcaag aacacagaga aagatatatt tctcaagatc agaagtacta
     4021 ttccagtatg gacgattcaa ggcttgcttc ataaaccaag gcaagtaata gagattggag
     4081 tctctaagaa agtagttcct actgaatcaa aggccatgga gtcaaaaatt cagatcgagg
     4141 atctaacaga actcgccgtg aagactggcg aacagttcat acagagtctt ttacgactca
     4201 atgacaagaa gaaaatcttc gtcaacatgg tggagcacga cactctcgtc tactccaaga
     4261 atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
     4321 tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag
     4381 tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc
     4441 aagatgcccc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
     4501 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
     4561 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
     4621 gttcatttca tttggagagg actccggtat ttttacaaca attaccacaa caaaacaaac
     4681 aacaaacaac attacaattt actattctag tcgaaatgaa tgaaattgac gaaaagaacc
     4741 aagcaccggt ccagcaagaa tgcctcaaag aaatgatcca aaacggtcat gcccgacgaa
     4801 tggggagtgt cgaggacctt tatgtagctc tcaatagaca aaacctgtac cgaaactttt
     4861 gcacatatgg agaactttca gactattgta cgcgagatca actcaccctg gctctccgtg
     4921 aaatttgttt gaagaatccg accctgcttc acatcgtgtt acctacgcgt tggccaaacc
     4981 acgaaaacta ctatcgttcc tccgaatatt actcaagacc gcatcccgtt cacgattaca
     5041 tctccgtttt gcaagaatta aaattaagcg gggtcgtact taatgaacaa cctgagtatt
     5101 cagctgttat gaagcaaatt ttagaagaat tcaaaaatag taagggttct tacacagcta
     5161 agatatttaa acttactaca actctcacga ttccttactt cggccctaca ggaccgtcat
     5221 ggcgattaat ttgtcttccc gaagagcata cggagaaatg gaagaaattc atattcgtta
     5281 gcaatcactg catgagtgat ggacgatcta gtatacactt ttttcatgat cttagagatg
     5341 agcttaataa cataaagact cctccaaaaa agctcgacta catcttcaag tacgaggagg
     5401 attatcaact cttaagaaag ctccccgagc caatagaaaa ggtgatcgac tttcgtcccc
     5461 catacctgtt tataccgaag agcctccttt ctggatttat ctataaccac ctgagattct
     5521 catcaaaggg agtctgtatg aggatggacg atgttgaaaa gacagatgat gttgtcacgg
     5581 aaattataaa cataagccca actgaattcc aggccataaa agcaaatatc aaatcaaaca
     5641 tccagggaaa gtgcacgata acaccttttt tacacgtttg ttggtttgtg agcttacaca
     5701 aatggggtaa atttttcaag cccctcaatt tcgagtggct tacagatata ttcatacctg
     5761 ccgattgcag atcacaactc cccgatgatg acgagatgcg tcagatgtat cgatatggtg
     5821 ccaatgtcgg gttcatagac ttcactccat ggatcagcga gttcgacatg aatgataata
     5881 aggagaattt ctggccgtta attgagcatt accacgaggt aattagtgaa gccttaagga
     5941 ataaaaagca cctccatggc ttagggttca acatccaagg tttcgttcag aagtatgtca
     6001 atatcgataa agtcatgtgc gatagggcta tcggcaaaag acgaggtggt acgctgttat
     6061 ctaatgtggg gttatttaat cagctggagg agccagacgc caaatacagt atctgtgatt
     6121 tggcatttgg ccaatttcag ggatcatggc atcaagcttt ctccttggga gtgtgttcta
     6181 ctaatgttaa gggcatgaac atagtggttg cctccacgaa aaatgtcgta ggcagtcaag
     6241 agtctttaga agagttgtgt tccatttata aggctcttct gctgggacca taggcttcgg
     6301 ccatgctaga gtccgcaaaa atcaccagtc tctctctaca aatctatctc tctctatttt
     6361 tctccagaat aatgtgtgag tagttcccag ataagggaat tagggttctt atagggtttc
     6421 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
     6481 atcaataaaa tttctaattc ctaaaaccaa aatccagtga cctcgcttga gacgaagctt
     6541 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
     6601 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
     6661 ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc
     6721 ttctgacgtt cagtgcagcc gtcatctgaa aacgacatgt cgcacaagtc ctaagttacg
     6781 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata
     6841 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct
     6901 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc
     6961 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc
     7021 gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg
     7081 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc
     7141 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg
     7201 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc
     7261 atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc
     7321 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc
     7381 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca
     7441 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag
     7501 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa
     7561 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg
     7621 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacctctca accgtgcggc
     7681 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg
     7741 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
     7801 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga
     7861 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc
     7921 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
     7981 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
     8041 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
     8101 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
     8161 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt
     8221 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
     8281 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
     8341 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
     8401 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
     8461 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
     8521 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
     8581 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
     8641 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
     8701 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
     8761 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
     8821 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgcctgccg
     8881 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca
     8941 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc
     9001 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
     9061 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg
     9121 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
     9181 gtgggcaccc gcgatagtcg cagcatcatg gacg
//
