LOCUS       sequence-377702-        9214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for expression of alcohol O-acetyltransferase 
            from Saccharomyces pastorianus strain CBS 1483 chromosome 
            SeVIII-SeXV, codon optimized for Nicotiana..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio
            S, Orzaez D, Patron N
  TITLE     Tunable control of insect pheromone biosynthesis in Nicotiana 
            benthamiana.
  JOURNAL   Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048.
  PUBMED    37032497
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048."
COMMENT     Sequence Label: GB3682
FEATURES             Location/Qualifiers
     source          1..9214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(67..89)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    175..315
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     230..249
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(330..347)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(501..1089)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(581..600)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1177..1992)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1177..1992)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1900..1919
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    2425..2449
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        3087..3431
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3375..3395
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    5329..5353
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6654..7283
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7712..8785
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8851..9045
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
ORIGIN
        1 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
       61 tgaaaacctc tgacacatgc agctcccggt gacggtcaca gcttgtctgt aagcggatgc
      121 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
      181 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
      241 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
      301 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
      361 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      421 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      481 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      541 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      601 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      661 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      721 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      781 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      841 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      901 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
      961 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1021 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1081 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1141 tcacgttaag ggattttggt catgcattct aggtgattag aaaaactcat cgagcatcaa
     1201 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     1261 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     1321 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     1381 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     1441 tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     1501 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagtc gaaatacgcg
     1561 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
     1621 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     1681 tttccctggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     1741 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     1801 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     1861 atacaatcgg tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     1921 atataaatca gcatccatgt tggaatttaa tcgcggcctt gagcaagacg tttcccgttg
     1981 aatatggctc ataacagaac ttattatttc cttcctcttt tctacagtat ttaaagatac
     2041 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
     2101 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
     2161 cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca
     2221 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
     2281 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
     2341 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
     2401 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca taacaagctt
     2461 cgtctcagtc aggagactag agccaagctg atctcctttg ccccggagat caccatggac
     2521 gactttctct atctctacga tctaggaaga aagttcgacg gagaaggtga cgataccatg
     2581 ttcaccaccg ataatgagaa gattagcctc ttcaatttca gaaagaatgc tgacccacag
     2641 atggttagag aggcctacgc ggcaggtctg atcaagacga tctacccgag taataatctc
     2701 caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt caggactaac
     2761 tgcatcaaga acacagagaa agatatattt ctcaagatca gaagtactat tccagtatgg
     2821 acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt ctctaagaaa
     2881 gtagttccta ctgaatcaaa ggccatggag tcaaaaattc agatcgagga tctaacagaa
     2941 ctcgccgtga agactggcga acagttcata cagagtcttt tacgactcaa tgacaagaag
     3001 aaaatcttcg tcaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat
     3061 acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac
     3121 ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa
     3181 ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcccct
     3241 gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac
     3301 gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat
     3361 gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat
     3421 ttggagagga ctccggtatt tttacaacaa ttaccacaac aaaacaaaca acaaacaaca
     3481 ttacaattta ctattctagt cgaaatgaat gaaatcgatg aaaaaaatca agctcccgta
     3541 caacaggagt gtctcaagga aatgatacag aacggccacg caaggaggat gggcagtgta
     3601 gaggacctct acgtagcctt aaacagacaa aacctgtata gaaacttttg tacgtatggg
     3661 gaactttccg actactgcac ccgagatcaa ctgacattag ccctgaggga gatttgtctc
     3721 aaaaatccaa cccttctgca tatagtcttg ccaacccgtt ggccaaacca tgagaactat
     3781 tatcgttctt ccgaatatta ttcaagaccc catcctgtcc atgattatat aagcgtactc
     3841 caagagttga agctttctgg ggtagtgctt aatgaacaac cggaatactc tgcagtgatg
     3901 aagcaaatcc ttgaagaatt taagaattca aaaggtagct atacggcaaa aatcttcaag
     3961 ttgacaacta ctttgacgat accctacttc gggcctaccg gtccttcatg gagattgatc
     4021 tgtctccccg aggaacatac agagaagtgg aggaagttca tttttgttag caaccactgt
     4081 atgtcagatg gcaggtccag tatacatttt ttccatgatc ttagagatga gcttaacaat
     4141 ataaagaccc ctccaaagaa gttagactat atctttaagt atgaagagga ttaccagtta
     4201 ttaaggaaac tccctgagcc tatagagaaa gttatagact ttaggccacc gtatttattc
     4261 atcccgaaga gccttcttag cggattcatc tataaccact tacgatttag ttccaaagga
     4321 gtgtgtatgc gaatggatga cgttgagaaa acggatgacg tcgtaacaga aataataaat
     4381 attagcccta cagaatttca agctatcaag gccaatatta agagtaacat ccaagggaaa
     4441 tgtacgatca cgcccttttt gcatgtgtgt tggtttgtat cccttcacaa gtggggaaaa
     4501 tttttcaagc cccttaactt cgaatggctg actgacattt ttataccagc tgattgcagg
     4561 tcccaactcc cagacgatga tgaaatgcga cagatgtata gatatggcgc taatgtaggg
     4621 tttatagact tcactccctg gataagcgag tttgatatga acgacaacaa agaaaacttc
     4681 tggcccttaa ttgaacacta ccacgaggtt atttctgaag ccttgaggaa taagaagcat
     4741 cttcatggcc ttggctttaa cattcaggga ttcgttcaaa agtatgtcaa tatagataag
     4801 gttatgtgcg accgtgctat tggaaaaaga cgtggcggta cgctcttgag caatgtgggt
     4861 ttgtttaatc aattggaaga gccggacgcc aaatattcta tatgcgactt agcctttggt
     4921 caatttcagg gaagctggca ccaagccttc agtcttggag tatgtagcac taacgtcaaa
     4981 ggaatgaaca tagtggttgc atcaactaaa aacgtggtag gatctcagga gagcttagag
     5041 gaattatgtt ccatttacaa agctctgctg ttagggcctt aggcttcggc catgctagag
     5101 tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt ctccagaata
     5161 atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt
     5221 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
     5281 ttctaattcc taaaaccaaa atccagtgac ctcgcttgag acgaagcttg acaggatata
     5341 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     5401 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     5461 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     5521 agtgcagccg tcatctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     5581 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     5641 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     5701 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     5761 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     5821 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     5881 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     5941 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     6001 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     6061 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     6121 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     6181 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     6241 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     6301 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     6361 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     6421 gcggccgggt acgtgttcga gccgcccgcg cacctctcaa ccgtgcggct gcatgaaatc
     6481 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     6541 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     6601 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     6661 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     6721 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     6781 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     6841 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     6901 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     6961 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     7021 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     7081 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     7141 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     7201 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     7261 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     7321 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     7381 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     7441 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     7501 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     7561 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     7621 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg
     7681 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
     7741 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
     7801 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
     7861 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
     7921 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
     7981 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
     8041 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
     8101 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
     8161 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
     8221 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
     8281 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
     8341 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
     8401 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
     8461 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
     8521 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
     8581 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
     8641 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
     8701 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
     8761 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
     8821 aggggaaaaa ggtcgaaaag gactctttcc tgtggatagc acgtacattg ggaacccaaa
     8881 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
     8941 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
     9001 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
     9061 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
     9121 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
     9181 caatctacca gggcgcggac aagccgcgcc gtcg
//
