LOCUS       sequence-377705-        8728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for expression of 
            1,2-diacyl-sn-glycerol:acetyl-CoA acetyltransferase from Euonymus 
            fortunei, codon optimized for Nicotiana..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio
            S, Orzaez D, Patron N
  TITLE     Tunable control of insect pheromone biosynthesis in Nicotiana 
            benthamiana.
  JOURNAL   Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048.
  PUBMED    37032497
REFERENCE   2  (bases 1 to 8728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048."
COMMENT     Sequence Label: GB3683
FEATURES             Location/Qualifiers
     source          1..8728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..195
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(431..453)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    539..679
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     594..613
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(694..711)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(865..1453)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(945..964)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1541..2356)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1541..2356)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2264..2283
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    2789..2813
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        3451..3795
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3739..3759
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    5207..5231
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6532..7161
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7590..8663
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
      361 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
      421 acggtgaaaa cctctgacac atgcagctcc cggtgacggt cacagcttgt ctgtaagcgg
      481 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
      541 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
      601 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
      661 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      721 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      781 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      841 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      901 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      961 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1021 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1081 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1141 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1201 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1261 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     1321 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1381 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1441 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1501 aaactcacgt taagggattt tggtcatgca ttctaggtga ttagaaaaac tcatcgagca
     1561 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     1621 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     1681 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     1741 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     1801 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     1861 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agtcgaaata
     1921 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     1981 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     2041 ctgttttccc tgggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     2101 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     2161 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     2221 tcccatacaa tcggtagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     2281 acccatataa atcagcatcc atgttggaat ttaatcgcgg ccttgagcaa gacgtttccc
     2341 gttgaatatg gctcataaca gaacttatta tttccttcct cttttctaca gtatttaaag
     2401 ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa
     2461 aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc gtataacata
     2521 gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt
     2581 tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg
     2641 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
     2701 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
     2761 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacataacaa
     2821 gcttcgtctc agtcaggaga ctagagccaa gctgatctcc tttgccccgg agatcaccat
     2881 ggacgacttt ctctatctct acgatctagg aagaaagttc gacggagaag gtgacgatac
     2941 catgttcacc accgataatg agaagattag cctcttcaat ttcagaaaga atgctgaccc
     3001 acagatggtt agagaggcct acgcggcagg tctgatcaag acgatctacc cgagtaataa
     3061 tctccaggag atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac
     3121 taactgcatc aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt
     3181 atggacgatt caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa
     3241 gaaagtagtt cctactgaat caaaggccat ggagtcaaaa attcagatcg aggatctaac
     3301 agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac tcaatgacaa
     3361 gaagaaaatc ttcgtcaaca tggtggagca cgacactctc gtctactcca agaatatcaa
     3421 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg
     3481 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa
     3541 ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
     3601 ccctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     3661 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
     3721 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     3781 tcatttggag aggactccgg tatttttaca acaattacca caacaaaaca aacaacaaac
     3841 aacattacaa tttactattc tagtcgaaat gatggacgtt caccaggaga ttaataattt
     3901 cattaaagtc tgggtgcaag ccatggcctg tcttagctat gcctactact tttcttcaag
     3961 gctcccaaaa ggtttattga ggctcctttc cctcctgccc gtattttact tactgcttat
     4021 tccacccttg aatatcagct cctttatact gagtagcata acggggttct tcttagcttg
     4081 gctcacaaca tttaaagtta tttcattcgc ttttgatcaa ggtcctctgt atcccctccc
     4141 gcagaatctc cttcatttca tttccattgc atgtctgccc ataacagtta agagaaaccc
     4201 aagtccaaaa ctcaaatcaa cgaccaacac gtcccctata agtcacatct taaagagcgt
     4261 atttatgtca ttcccttcta aggtgctcct gcactggata attgctcatc tctatcaata
     4321 caaaaagtac atggacccta atgtggtgct tgtcatttat tgctgtcatg tttacgtgct
     4381 cctggacatt agcttgtccc tttgcgctac tctcgcagaa tttctgtgcg gatttgacgt
     4441 tgaacctcag ttcaaggaac cctacctcgc tacatctctc caggactttt ggggtcgtag
     4501 atggaatatt attgtgtcat ctgtgctgcg tagtacggtc tataccccga ccaggcacat
     4561 agcttcttat cttataggct ccaggtgggc atactttcca gccattatag ctacctttgt
     4621 tgtgtcaggt gttatgcacg atatcgttta ttacgtatac atgatgcacg tttacccaaa
     4681 atgggacatg acaggtcatt ttgtactgca cggcatttgt gaagcactgg aagtagagat
     4741 gaagtgtaag agaagccgtc gagacaaatg gcgaagacat ccagtcgtag attgggttat
     4801 ggttatgggc tttgtgatgg gcacttcagt tagtctttta tttgtcccat tgctccgtga
     4861 taatgtagac caaatagtgg ccgaggagtg ctccatcctg ttgaatttcg tccgagagaa
     4921 gattgtcatg ctggggaccc gttttatatg tggaaattga gcttcggcca tgctagagtc
     4981 cgcaaaaatc accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat
     5041 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     5101 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     5161 ctaattccta aaaccaaaat ccagtgacct cgcttgagac gaagcttgac aggatatatt
     5221 ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt
     5281 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg
     5341 atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag
     5401 tgcagccgtc atctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg
     5461 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt
     5521 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct
     5581 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg
     5641 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc
     5701 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc
     5761 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg
     5821 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg
     5881 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc
     5941 ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca
     6001 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag
     6061 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg
     6121 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg
     6181 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca
     6241 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc
     6301 ggccgggtac gtgttcgagc cgcccgcgca cctctcaacc gtgcggctgc atgaaatcct
     6361 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac
     6421 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
     6481 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
     6541 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
     6601 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
     6661 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
     6721 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
     6781 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
     6841 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
     6901 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
     6961 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
     7021 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
     7081 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
     7141 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
     7201 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
     7261 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
     7321 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
     7381 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
     7441 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
     7501 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg cctgccggcc ctgcaatggc
     7561 actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac
     7621 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
     7681 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
     7741 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
     7801 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
     7861 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
     7921 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
     7981 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
     8041 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
     8101 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
     8161 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
     8221 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
     8281 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
     8341 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
     8401 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
     8461 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
     8521 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
     8581 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
     8641 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
     8701 gggaaaaagg tcgaaaagga ctctttcc
//
