LOCUS       sequence-377708-       14043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for plant responses to bacterial infection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14043)
  AUTHORS   Zhu J, Lolle S, Tang A, Guel B, Kvitko B, Cole B, Coaker G
  TITLE     Single-cell profiling of Arabidopsis leaves to Pseudomonas syringae 
            infection.
  JOURNAL   Cell Rep. 2023 Jun 20;42(7):112676. doi: 
            10.1016/j.celrep.2023.112676.
  PUBMED    37342910
REFERENCE   2  (bases 1 to 14043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-06-20"; volume: "42(7):112676. doi"; pages: " 
            10.1016/j.celrep.2023.112676"
COMMENT     Sequence Label: pCBP60g-NLS-3xmCitrine
FEATURES             Location/Qualifiers
     source          1..14043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..347
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2555..2575
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(2592..2616)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2648..2650,2651..2653,2654..3364)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3167..3904
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="DGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKAGGSGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVS
                     GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMP
                     EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
                     NVYIMADKQKNGIKVNFKIRHNIE"
     CDS             join(3386..3388,3389..3391,3392..4102)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3905..4642
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="DGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKAGGSGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVS
                     GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMP
                     EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
                     NVYIMADKQKNGIKVNFKIRHNIE"
     CDS             join(4124..4126,4127..4129,4130..4840)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      4903..5155
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5188..5204)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5188..5204)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5201..5223)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5212..5228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5236..5242,5243..5260,5261..5266))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5281..5302
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    5387..5561
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5602..6627)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(6694..7370)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6737..6757)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    7565..7589
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8110..8901
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9147..9735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9636..9655
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9889..9906
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9921..10061
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9987..10006)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10147..10169
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10405..10599
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10665..11738)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12167..12796)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gcaggctccg cggccgcccc
      361 cttcacctgg ctcgatcaaa cttagatatc aatcaacatt tagtttgact attcggcctc
      421 tctttagttt ccttacgatt ttcctcgaat ttggaaggaa atgttctctc aacataaaac
      481 catcaattat ttcttttaag ttttaacgcc aatttttaat tgccatctag aaaaatatct
      541 tatacaccag aaaatgatca tgtccgcact actataggat acttctatct gattaatttt
      601 attagactta tcataatgaa tgttttgaat aaatttgata atcatatgta ttaattagaa
      661 ttatacacta ctaatctcgt gttttaaaac atgaaaaaga ccaaaaaggg attaaaaaaa
      721 aaattatttg tttgagtaca cgaacaaaat aggctaacga caatccaacc tcaaaaggac
      781 atgattcgat acattaccaa aagatagttg tcaacggttt ttctgaaaca atcatattaa
      841 agtatgatta gttcttttaa aaaccaaaaa ttatattata aaagataaaa tatttttaca
      901 aaaacaaaca atagattttg aacctttaaa ttattgttct aattgaccgg tgaacataac
      961 tactcaacat atataatatt atttcctcta gtatagttat tttttaaaaa atctatttta
     1021 ttttaatttc tagaaaatat ttattatttt taatctattt tattttaatt tctataaatt
     1081 ctgaaccttg aaaactacat tgtttattgg caaaaaaaaa aaaaatcgac gtgtacactt
     1141 ctcccacatg aaatatctac ctgttaaaaa ttatatacaa atattaatac tatatttgtc
     1201 ctttcagttt tttttttcct cttagataac cgtttgtgtc gttttgtttg gtagattatc
     1261 aaagaagaag attgtccatc agcagaccgc gtcgatgtag aaaaacatgt tactttagtt
     1321 ttctgaatat ctccttccaa ttttgaaacc attatgttga atcttaatat tttgatcata
     1381 tcaattactg aaaaacgcgt taacggcatc acctaagcgt ggcttttttg ggggtcaatg
     1441 tacttctagt tgacgatcta gccatgtcaa cgcttatgtc aacggctgtt ttggctagtt
     1501 tatggaattt caaataattc tgtatttgaa atgttcataa ttcattcacc taattagacc
     1561 aagataatgt catataggta aaaatctaat tatagtttta attataacta gatttggaag
     1621 tttttatctt tactgttatt ctatatttta gatgttatga acaaaaattt ggtgaattat
     1681 ccaattaaat tctcttattg tatataaaaa tacatagtaa tctgaaaaac atatattttc
     1741 atttgaaaac actttaaaat atttaaaccc tggtatgttt tgcaaaaaaa aatataaaat
     1801 aagatactga tttaaaacaa atcaccaaat cttcattacc aaaacataaa aatgtattca
     1861 tcagatttaa aataaagttg caacacatgc agttaatata atctcatatg atctctccaa
     1921 tactagattc catcgttgat ataaaccggt acttgttctt cagcaaagaa aaactgagaa
     1981 tactgctaat ggtataccca atatcacaac atgttaccaa ctagaaccag ttttaataca
     2041 acgatcctta ctttaagtta cataaacaaa aaattaatac actgaaatta tttaatctct
     2101 ccactatatc cgacttttcc aatatgagag gaagctaatt aaaatacatg tagaggttta
     2161 gtttgatcga gtaatatatt cttctatatt attacaagct aattttagtt tgtacgaaat
     2221 gatccaaaca gcaattaaat aacgaactaa tatatgacac aacacaacac aacacaagtg
     2281 tctttatttt tgattttaat tagttaacta aataactaac tctagcgtgg ttaatgaata
     2341 gcacttataa actccgagca cgaatcttcc tggaagtttc actgctgctt cgtcaatact
     2401 cattgccttt atattttcct cattcactct cttcccattc tcatctctct ctctctacct
     2461 ttctctcaca caaagagttt tttcttttaa gacaaaaact ctttcaaggt tttaaatctt
     2521 tgaaaaaatt ctctaaacct aaaagtgatc aatgccaaag aagaagagaa aggtcaaggg
     2581 tgggcgcgcc gacccagctt tcttgtacaa agtggttgat aacagcgctg gaggctccgg
     2641 tgggagcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2701 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2761 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2821 gcccaccctc gtgaccacct tcggctacgg cctgatgtgc ttcgcccgct accccgacca
     2881 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2941 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3001 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3061 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3121 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3181 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3241 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3301 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3361 caaggctgga ggctccggtg ggagcatggt gagcaagggc gaggagctgt tcaccggggt
     3421 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     3481 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     3541 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgatgtgctt
     3601 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3661 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3721 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3781 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     3841 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     3901 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3961 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     4021 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     4081 cggcatggac gagctgtaca aggctggagg ctccggtggg agcatggtga gcaagggcga
     4141 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     4201 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     4261 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg
     4321 ctacggcctg atgtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     4381 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     4441 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     4501 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     4561 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     4621 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     4681 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     4741 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     4801 cgccgccggg atcactctcg gcatggacga gctgtacaag taagcttgca tgcctgcagg
     4861 tcgactctag aggatccccg ggtaccgagc tcgaatttcc ccgatcgttc aaacatttgg
     4921 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     4981 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     5041 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     5101 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
     5161 ttggttccgg aaccaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     5221 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5281 atgagtgagc taactcacat taggctgaat taggcgcgcc tatttctgaa ttcaacacat
     5341 tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcgggggatc tggattttag
     5401 tactggattt tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa
     5461 atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa
     5521 ttcccttatc tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac
     5581 agatcccggt cggcatctac tctatttctt tgccctcgga cgagtgctgg ggcgtcggtt
     5641 tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc
     5701 gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg
     5761 cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc
     5821 aatgcggagc atatacgccc ggagtcgtgg cgatcctgca agctccggat gcctccgctc
     5881 gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc
     5941 gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg
     6001 gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc
     6061 ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac
     6121 ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa
     6181 atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt
     6241 ctggctaaga tcggccgcag cgatcgcatc catagcctcc gcgaccggtt gtagaacagc
     6301 gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgaacggc gggagatgca
     6361 ataggtcagg ctctcgctaa actccccaat gtcaagcact tccggaatcg ggagcgcggc
     6421 cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac
     6481 ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc
     6541 cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga
     6601 cgtcgcggtg agttcaggct ttttcatatc tcattgcccc ccggatctgc gaaagctcga
     6661 gagagataga tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga
     6721 acttccttat atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac
     6781 gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt
     6841 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc
     6901 ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc
     6961 tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga
     7021 tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat
     7081 attcttggag tagacgagag tgtcgtgctc caccatgttc acatcaatcc acttgctttg
     7141 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
     7201 tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc
     7261 atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc
     7321 aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt
     7381 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca
     7441 tgttggcaag ctgctctagc caatacgcaa accgaattca gaaataaatt cagcctaatt
     7501 cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca
     7561 atttgtttac accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc
     7621 tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga
     7681 gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa
     7741 ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg
     7801 atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctgt
     7861 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
     7921 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     7981 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     8041 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     8101 ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     8161 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     8221 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     8281 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     8341 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     8401 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     8461 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     8521 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     8581 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     8641 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     8701 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     8761 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     8821 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
     8881 accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc
     8941 ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat
     9001 caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga
     9061 gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9121 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     9181 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     9241 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     9301 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9361 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9421 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9481 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9541 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9601 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9661 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9721 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9781 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     9841 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     9901 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     9961 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
    10021 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
    10081 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
    10141 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
    10201 agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct
    10261 ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga
    10321 cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct
    10381 tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag agttttaata
    10441 agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac
    10501 atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca
    10561 atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct
    10621 ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat
    10681 gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc
    10741 tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa
    10801 cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat
    10861 ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg
    10921 ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg
    10981 atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg
    11041 ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg
    11101 ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag
    11161 gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg
    11221 tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg
    11281 ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg
    11341 gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc
    11401 acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg
    11461 cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg
    11521 cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg
    11581 cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt
    11641 tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg
    11701 ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg
    11761 ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg
    11821 ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc
    11881 cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt
    11941 agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac
    12001 atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc
    12061 gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca
    12121 cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg
    12181 atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt
    12241 caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg
    12301 actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg
    12361 tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa
    12421 tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct
    12481 gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct
    12541 ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa
    12601 tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat
    12661 cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca
    12721 gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta
    12781 cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata
    12841 cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg
    12901 gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa
    12961 accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta
    13021 cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc
    13081 gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg
    13141 tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc
    13201 acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca
    13261 gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt
    13321 gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc
    13381 ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg
    13441 gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc
    13501 aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg
    13561 gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc
    13621 ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg
    13681 cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg
    13741 cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc
    13801 tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag
    13861 ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac
    13921 ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta
    13981 ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa
    14041 cct
//
