LOCUS       sequence-377709-       14200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for plant responses to bacterial infection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14200)
  AUTHORS   Zhu J, Lolle S, Tang A, Guel B, Kvitko B, Cole B, Coaker G
  TITLE     Single-cell profiling of Arabidopsis leaves to Pseudomonas syringae 
            infection.
  JOURNAL   Cell Rep. 2023 Jun 20;42(7):112676. doi: 
            10.1016/j.celrep.2023.112676.
  PUBMED    37342910
REFERENCE   2  (bases 1 to 14200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-06-20"; volume: "42(7):112676. doi"; pages: " 
            10.1016/j.celrep.2023.112676"
COMMENT     Sequence Label: pEXPA10-NLS-3xmCitrine
FEATURES             Location/Qualifiers
     source          1..14200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..347
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2712..2732
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(2749..2773)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2805..2807,2808..2810,2811..3521)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3324..4061
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="DGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKAGGSGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVS
                     GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMP
                     EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
                     NVYIMADKQKNGIKVNFKIRHNIE"
     CDS             join(3543..3545,3546..3548,3549..4259)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4062..4799
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="DGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKAGGSGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVS
                     GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMP
                     EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
                     NVYIMADKQKNGIKVNFKIRHNIE"
     CDS             join(4281..4283,4284..4286,4287..4997)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      5060..5312
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5345..5361)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5345..5361)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5358..5380)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5369..5385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5393..5399,5400..5417,5418..5423))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5438..5459
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    5544..5718
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5759..6784)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(6851..7527)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6894..6914)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    7722..7746
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8267..9058
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9304..9892
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9793..9812
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10046..10063
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10078..10218
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10144..10163)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10304..10326
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10562..10756
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10822..11895)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12324..12953)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gcaggctccg cggccgcccc
      361 cttcacccgt atagataata attaaatgaa tcctgtggag gtgaacataa cacacaatgt
      421 tgatagaatt tgtcatcaac aggtggatag tcgcatggac aaacatagag aggttttgga
      481 aggggcactt tgctccccat ggttttctcg aacttaccaa gaaaaataaa agacttttaa
      541 ggttcacctg ttttctttag aaggatcttt aataagtatt tgaaaatgtt actaatctaa
      601 attaatgtgc aattacagac aaacaaacaa aaaacgaaga gaagcgaaat taaaatagga
      661 gaccagtacg agcggaaacg atgaaaaaga caaaacagca ggcatgtgca tgcattggct
      721 ttggctattg atctcaagct taataaaaaa tgttagaaac tatccctttc aattttcaat
      781 actccttcac tatataccaa ataaccaatt tgatgactag cagaaaaata ttatatttgt
      841 ttggcttctt tcaaaccccc gattaattta ttttggcgtt tgtaaactca aggaatccta
      901 tgatattatt ttttacgttg cgatttcgag tttctcctat gattgttaaa ttttaatttc
      961 ttttgtggtt acacgcatgc tggatattat gctttagtaa tgtgtagtca atttttatta
     1021 cttatcattt aatccccatg acactagctt gataaatcga cagatatata ttatatatgt
     1081 aatgaatgta agaggataat caataatttt ttggcgttaa tctaatcatt tctagataca
     1141 agcgttacga acaattcaaa tgatcaaact ttccttcttt tgctatttta tcataaataa
     1201 aacgttagcc taaacggtgt ttgtcgacta cttgtggtcc tctggcaaat ctatgtactc
     1261 atcgtctcat tctctttatt ttctatcatt tttgttgagt tcttattttt ttgtttgaca
     1321 ctcgaaaaaa tgttgagaag taacgtaacc gactttgcat tgcttttgtt aaaccatcaa
     1381 aatgaattga aggttatgta aacaaaagta gtagcaaggg cccatgtttt tgagctctga
     1441 ttacgggagg ttggccgtag atttctgcgg ctgcaagttc ttgaccttat taaattcaat
     1501 agttacaatt atcttctcgt cgaaaatttc tgatatattt attttttttg aattaaatag
     1561 gaactggtta cataacacgc acgatatagg taggaaaaca caggtaagga acagagttat
     1621 aggtttcctg atacgtctat ctcaaaacag accactgtga cccacttaga tttcagcttg
     1681 atttttctat cactgttttt gcttcttttt tacttttttc ccctagttca gagttctaaa
     1741 attgtaaatt agttttatcg tagaagtacc aaatcaagtg attcaatggt taaattaagg
     1801 tattaagtta catttgatat ttaaaagtat ccagaccttc attatagctc ataagggtta
     1861 acattttgtc gttcttttgt atattcatgg caagctctaa ttcatgacta agtcacattt
     1921 ttcaaatatg tttttagttt ttacttatgt tggtaattag tggatttata gttaagttaa
     1981 aaagttggcg agttctagct ttgaaactca tttagatata tatatatata tatatatata
     2041 ttcaatttta gtaaattgtt aatctattct aatggtgtaa ctgtaacaaa tgagaatgaa
     2101 aaaaatatac tattgtgaat aaaaccccac acaacacatt actataataa gttaaacttc
     2161 tttttttgta ggcgcctgga aaaaaaagaa aagcaacaag agggctgtga ggacgcatca
     2221 ccggtttcgt agcacacatg tgcatttgtc tctttgcttt ttcggttttt ttcttgccaa
     2281 tcaatttatt ttgttcctca gaaaaaagaa aatctaaaac caaaatatat attataacct
     2341 catttaataa acaacaaaaa tgtttgttga aaaaaaaaaa gtttttattt atcttgacct
     2401 tatttctttg aagaaaataa agcttggtta ttaaagaagt ccaagttagt tgccaccatc
     2461 agtggcataa cggtaaatta aagccaactt cctctaacta aagttttcta taaattcaac
     2521 cactcacctc ccactctaaa acccaacaac ataatttcac atatctctct ttctttctct
     2581 tgaaggaaag acgaagatct ccaagtccca agtacgtaac tactttctcc atctacattc
     2641 aattgtttct ccttaatttc tctagtacat atttacttgt gctataagta attgatttta
     2701 tatcacccat gccaaagaag aagagaaagg tcaagggtgg gcgcgccgac ccagctttct
     2761 tgtacaaagt ggttgataac agcgctggag gctccggtgg gagcatggtg agcaagggcg
     2821 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     2881 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     2941 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg
     3001 gctacggcct gatgtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     3061 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     3121 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     3181 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     3241 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     3301 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     3361 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
     3421 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     3481 ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggctggaggc tccggtggga
     3541 gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     3601 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     3661 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     3721 ccctcgtgac caccttcggc tacggcctga tgtgcttcgc ccgctacccc gaccacatga
     3781 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     3841 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     3901 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     3961 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     4021 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     4081 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     4141 actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     4201 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     4261 ctggaggctc cggtgggagc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     4321 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     4381 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     4441 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgatg tgcttcgccc
     4501 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     4561 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     4621 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     4681 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     4741 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     4801 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     4861 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
     4921 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     4981 tggacgagct gtacaagtaa gcttgcatgc ctgcaggtcg actctagagg atccccgggt
     5041 accgagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     5101 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     5161 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     5221 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     5281 atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg gttccggaac caattcgtaa
     5341 tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     5401 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattag
     5461 gctgaattag gcgcgcctat ttctgaattc aacacattgc ggacgttttt aatgtactga
     5521 attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg ttttaggaat
     5581 tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg gtttcttata
     5641 tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg gaactactca
     5701 cacattatta tggagaaact cgagcttgtc gatcgacaga tcccggtcgg catctactct
     5761 atttctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta
     5821 cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc
     5881 cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat
     5941 tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga
     6001 gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca
     6061 tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga
     6121 acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt
     6181 tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca
     6241 tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc
     6301 agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac
     6361 cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga
     6421 tcgcatccat agcctccgcg accggttgta gaacagcggg cagttcggtt tcaggcaggt
     6481 cttgcaacgt gacaccctgt gaacggcggg agatgcaata ggtcaggctc tcgctaaact
     6541 ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat
     6601 aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc
     6661 ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga
     6721 cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt
     6781 tcatatctca ttgccccccg gatctgcgaa agctcgagag agatagattt gtagagagag
     6841 actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata gaggaagggt
     6901 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
     6961 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
     7021 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
     7081 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
     7141 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
     7201 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
     7261 cgtgctccac catgttcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct
     7321 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca
     7381 tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt
     7441 tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc
     7501 gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg
     7561 atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggcaagctg ctctagccaa
     7621 tacgcaaacc gaattcagaa ataaattcag cctaattcgg cgttaattca gtacattaaa
     7681 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
     7741 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
     7801 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
     7861 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
     7921 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
     7981 atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     8041 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     8101 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     8161 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     8221 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt
     8281 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     8341 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     8401 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     8461 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     8521 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     8581 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     8641 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     8701 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     8761 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     8821 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     8881 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     8941 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     9001 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg
     9061 tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta
     9121 gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat
     9181 ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat
     9241 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9301 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9361 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9421 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     9481 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9541 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9601 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9661 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     9721 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9781 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9841 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9901 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9961 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    10021 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
    10081 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
    10141 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
    10201 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
    10261 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
    10321 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg
    10381 gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc
    10441 agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga
    10501 gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag
    10561 ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga
    10621 aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta
    10681 cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat
    10741 gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg
    10801 ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg
    10861 gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg
    10921 caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc
    10981 gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc
    11041 gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg
    11101 gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc
    11161 tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct
    11221 aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg
    11281 ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg
    11341 ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac
    11401 gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga
    11461 aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga
    11521 aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg
    11581 cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc
    11641 atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga
    11701 cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg
    11761 ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc
    11821 cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg
    11881 tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
    11941 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
    12001 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
    12061 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
    12121 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
    12181 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
    12241 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
    12301 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
    12361 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
    12421 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
    12481 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
    12541 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
    12601 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
    12661 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
    12721 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
    12781 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
    12841 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
    12901 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
    12961 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
    13021 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
    13081 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
    13141 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
    13201 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
    13261 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
    13321 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
    13381 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
    13441 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
    13501 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
    13561 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
    13621 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
    13681 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
    13741 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
    13801 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
    13861 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
    13921 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
    13981 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
    14041 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
    14101 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg
    14161 tggttggcat gcacatacaa atggacgaac ggataaacct
//
