LOCUS       sequence-377710-       13859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for plant responses to bacterial infection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13859)
  AUTHORS   Zhu J, Lolle S, Tang A, Guel B, Kvitko B, Cole B, Coaker G
  TITLE     Single-cell profiling of Arabidopsis leaves to Pseudomonas syringae 
            infection.
  JOURNAL   Cell Rep. 2023 Jun 20;42(7):112676. doi: 
            10.1016/j.celrep.2023.112676.
  PUBMED    37342910
REFERENCE   2  (bases 1 to 13859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-06-20"; volume: "42(7):112676. doi"; pages: " 
            10.1016/j.celrep.2023.112676"
COMMENT     Sequence Label: pLipoP1-NLS-3xmCitrine
FEATURES             Location/Qualifiers
     source          1..13859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..347
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2371..2391
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(2408..2432)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2464..2466,2467..2469,2470..3180)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2983..3720
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="DGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKAGGSGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVS
                     GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMP
                     EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
                     NVYIMADKQKNGIKVNFKIRHNIE"
     CDS             join(3202..3204,3205..3207,3208..3918)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3721..4458
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="DGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKAGGSGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVS
                     GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMP
                     EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
                     NVYIMADKQKNGIKVNFKIRHNIE"
     CDS             join(3940..3942,3943..3945,3946..4656)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      4719..4971
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5004..5020)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5004..5020)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5017..5039)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5028..5044
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5052..5058,5059..5076,5077..5082))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5097..5118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    5203..5377
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5418..6443)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(6510..7186)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6553..6573)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    7381..7405
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7926..8717
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8963..9551
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9452..9471
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9705..9722
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9737..9877
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9803..9822)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9963..9985
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10221..10415
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10481..11554)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11983..12612)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gcaggctccg cggccgcccc
      361 cttcaccaga agctgtacaa gaaagtcgat acgaatgttc ttagtaaaat cccaagagga
      421 actgtcaaaa acaagaaatt tacttaattc tgatcattct ttacttatag agataatgtg
      481 tttgctactg tatttatgcg acctcatcaa cccaaattaa tatacatatc tgttaaaaga
      541 agaagaagaa gttaagatct gataatcatc atcaaatgga ataatgatgt gaaattgtga
      601 aaatcaagcc tctagggaaa taattaacaa gaacttgttt atggtaactt tctccaatta
      661 atgatattgg tctagtttta accttgatga gtataataat aaactataaa tcgaccaaat
      721 cacttgaaaa gcgggtatca tcagcacatc gctgcaaatc tgttgcacat tatatcacca
      781 tcacattcct aaaacctgtt ataacgttaa gatttgccaa aaccgagcta tttttttttc
      841 aataataatt ttcttctata aaaataaaat aaataaacac taaatcgaaa gcgaaagtgg
      901 tcggataaat atcaagatac gtacgtttgt ttacatgtat gggaaaaaat acaaaacaaa
      961 ataccaaatt ttcgtttttt gtccaaacca aactttgtct aaaatagata ccaattgttc
     1021 tacaaaattg tcaaagagat attttgttat tttgaaaatg acaatgttaa taataagtgg
     1081 agttagactt ttcacttttg ttttaaccta caatttctct ctcgcttaac atcgactatt
     1141 ctaacacatt tttcaatctt cctttttatt aaggaacttt aatttttcca aaaatctgaa
     1201 acatactata cccaaaaatc gatgctcaca cctagctagt tcaaactgcg tacgtcaaaa
     1261 tatgtggtat ggacgtaaag tattttataa gttttgttag aaagttagat acttataaaa
     1321 taaaaaaatt gatatttttt tggtaaaaaa tggaagtttg gtattttttg gcagttgtat
     1381 acataaataa aaatatatat tacggagtta tatattcttg tttggtcaaa tgtttcggaa
     1441 gcttttagat tacgaaatta cataatccat gatgaattaa atttggttag tggtagaaga
     1501 ataagacgcc aaaagaaaaa agggaagaac attgggttac gtcgaaagtc ctttgataag
     1561 aatttgattt tgcatagtca aatttggacc aacaacaaaa atggagtcca cgtgaaatag
     1621 aagagagaga cttaatagct tctcatgcat aaagttatga acaatcaatg ataaccaatg
     1681 ataaataact aaacagtaca aaatctctgt atttttattg gatttaacaa aggccatgaa
     1741 caatcacttc aatatttata atttttttaa aaagacgagg caattcaagc tgatttatac
     1801 attatgatta atgataatta tattatggca tttgactttt ctcacgctaa tgtaaatggt
     1861 caaaaatcct tatcttcaac taaacttcct cgacaaaacc ttaactagaa ctatgtatgt
     1921 aatttatcaa atattgaact ttaaatttct ttaaaagcat aatataatct tgacctataa
     1981 aattagccta gcctttcgaa ttacaagttt tatattttta aagaaacata atcttcaact
     2041 agaaattatt ataaaccggg tcctatcttc atctaatata cgtgattcca tcaaaattcc
     2101 gcagtcaaat gtgtttagtt gagaggagga gaatagtaga ctaaaatgga tgactttctg
     2161 gtaagtcttg tattgcagtc ttttctatat tttaacaaat aagtcttctt ttcttaaaaa
     2221 gaaaaattta attacaaaga aatcttacta ctgaacgaat aatttatcaa aagtcagtgg
     2281 ccttacgtac gaatttatcg gctataagta ggaaagcttt tcacgttaaa caacttaatc
     2341 tcatccacat taactagaga aagagagatg ccaaagaaga agagaaaggt caagggtggg
     2401 cgcgccgacc cagctttctt gtacaaagtg gttgataaca gcgctggagg ctccggtggg
     2461 agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     2521 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     2581 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     2641 accctcgtga ccaccttcgg ctacggcctg atgtgcttcg cccgctaccc cgaccacatg
     2701 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     2761 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     2821 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     2881 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     2941 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     3001 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     3061 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     3121 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     3181 gctggaggct ccggtgggag catggtgagc aagggcgagg agctgttcac cggggtggtg
     3241 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     3301 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     3361 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgat gtgcttcgcc
     3421 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     3481 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     3541 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     3601 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     3661 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     3721 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     3781 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac
     3841 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3901 atggacgagc tgtacaaggc tggaggctcc ggtgggagca tggtgagcaa gggcgaggag
     3961 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     4021 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     4081 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac
     4141 ggcctgatgt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     4201 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     4261 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     4321 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     4381 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     4441 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     4501 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc
     4561 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     4621 gccgggatca ctctcggcat ggacgagctg tacaagtaag cttgcatgcc tgcaggtcga
     4681 ctctagagga tccccgggta ccgagctcga atttccccga tcgttcaaac atttggcaat
     4741 aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt
     4801 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
     4861 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
     4921 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattgg
     4981 ttccggaacc aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5041 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5101 gtgagctaac tcacattagg ctgaattagg cgcgcctatt tctgaattca acacattgcg
     5161 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
     5221 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
     5281 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
     5341 cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg atcgacagat
     5401 cccggtcggc atctactcta tttctttgcc ctcggacgag tgctggggcg tcggtttcca
     5461 ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt
     5521 tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc
     5581 caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg
     5641 cggagcatat acgcccggag tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag
     5701 tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc
     5761 tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca
     5821 ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg
     5881 cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg
     5941 tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca
     6001 cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg
     6061 ctaagatcgg ccgcagcgat cgcatccata gcctccgcga ccggttgtag aacagcgggc
     6121 agttcggttt caggcaggtc ttgcaacgtg acaccctgtg aacggcggga gatgcaatag
     6181 gtcaggctct cgctaaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat
     6241 gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc
     6301 aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag
     6361 agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc
     6421 gcggtgagtt caggcttttt catatctcat tgccccccgg atctgcgaaa gctcgagaga
     6481 gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt
     6541 ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca
     6601 gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
     6661 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
     6721 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
     6781 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
     6841 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
     6901 ttggagtaga cgagagtgtc gtgctccacc atgttcacat caatccactt gctttgaaga
     6961 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
     7021 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
     7081 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
     7141 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
     7201 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
     7261 ggcaagctgc tctagccaat acgcaaaccg aattcagaaa taaattcagc ctaattcggc
     7321 gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
     7381 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
     7441 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
     7501 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
     7561 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
     7621 cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg
     7681 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     7741 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     7801 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     7861 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     7921 acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     7981 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     8041 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     8101 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     8161 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     8221 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     8281 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     8341 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     8401 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     8461 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     8521 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     8581 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     8641 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     8701 aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg
     8761 cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg
     8821 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata
     8881 tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8941 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9001 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9061 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     9121 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9181 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9241 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9301 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9361 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9421 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9481 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9541 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9601 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9661 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9721 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     9781 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     9841 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     9901 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     9961 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
    10021 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
    10081 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
    10141 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
    10201 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
    10261 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
    10321 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
    10381 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
    10441 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
    10501 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
    10561 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
    10621 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
    10681 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
    10741 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
    10801 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
    10861 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
    10921 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
    10981 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
    11041 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
    11101 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
    11161 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
    11221 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
    11281 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
    11341 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
    11401 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
    11461 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
    11521 tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc
    11581 agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc
    11641 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
    11701 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
    11761 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
    11821 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
    11881 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
    11941 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
    12001 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
    12061 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
    12121 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
    12181 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
    12241 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
    12301 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
    12361 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
    12421 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
    12481 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
    12541 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
    12601 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
    12661 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
    12721 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
    12781 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
    12841 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
    12901 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
    12961 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
    13021 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
    13081 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
    13141 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
    13201 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
    13261 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
    13321 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
    13381 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
    13441 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
    13501 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
    13561 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
    13621 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
    13681 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
    13741 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
    13801 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacct
//
