LOCUS       sequence-377711-       13947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for plant responses to bacterial infection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13947)
  AUTHORS   Zhu J, Lolle S, Tang A, Guel B, Kvitko B, Cole B, Coaker G
  TITLE     Single-cell profiling of Arabidopsis leaves to Pseudomonas syringae 
            infection.
  JOURNAL   Cell Rep. 2023 Jun 20;42(7):112676. doi: 
            10.1016/j.celrep.2023.112676.
  PUBMED    37342910
REFERENCE   2  (bases 1 to 13947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-06-20"; volume: "42(7):112676. doi"; pages: " 
            10.1016/j.celrep.2023.112676"
COMMENT     Sequence Label: pILL5-NLS-3xmCitrine
FEATURES             Location/Qualifiers
     source          1..13947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..347
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2459..2479
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(2496..2520)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2552..2554,2555..2557,2558..3268)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3071..3808
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="DGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKAGGSGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVS
                     GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMP
                     EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
                     NVYIMADKQKNGIKVNFKIRHNIE"
     CDS             join(3290..3292,3293..3295,3296..4006)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3809..4546
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="DGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKAGGSGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVS
                     GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMP
                     EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
                     NVYIMADKQKNGIKVNFKIRHNIE"
     CDS             join(4028..4030,4031..4033,4034..4744)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      4807..5059
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5092..5108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5092..5108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5105..5127)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5116..5132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5140..5146,5147..5164,5165..5170))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5185..5206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    5291..5465
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5506..6531)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(6598..7274)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6641..6661)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    7469..7493
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8014..8805
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9051..9639
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9540..9559
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9793..9810
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9825..9965
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9891..9910)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10051..10073
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10309..10503
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10569..11642)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12071..12700)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gcaggctccg cggccgcccc
      361 cttcacctct cacagtatta gtttcttctg tgttataggc tgtggcatgg ggagagatga
      421 gtatgggtga agcagagagg aggcttttgg ctaatgcgtt gcttgatatc tctaatgaat
      481 ggtttgttct gctgtctgaa tcatgcattc ctctgcgagg tttcagcttt atctatagtt
      541 atgtctctga atcaagatat agttttatgg gtgctgctga tgaggaagga cctgatggaa
      601 gaggcagata caggacagaa atggaacctg agatcacact aagccagtgg cgaaaagggt
      661 ctcagtggtt tgaaattaac cggaaactcg ctgttgagat tgttcaagac actacttact
      721 atcccaaatt caaagagttt tgtagacctc catgctatgt agatgaacat tacttcccaa
      781 caatgttgtc tatgaaacat cgggtttgct tgctaatagg acattgacat ggacagattg
      841 gtcacgtggt ggtgctcatc cagctacttt tggtaaggct gatgtaacgg aaagttttct
      901 caagaagctt acaggagcca aatcttgcct ctacaatgat catcagtctc aaatctgtta
      961 tctctttgcc agaaagtttg ctccaagcgc attggagcct ttactgcaac ttgcacccaa
     1021 gattcttgag ttaggctcta acatatcttc cactcgtcac tcttggtatt tgttttgaga
     1081 agttcttgag tttagagtat gatcatggac cgaactgatc attagttaat ctcctctttc
     1141 acaatgatat tacaggtatt gtgaaggtta aacatatttt ttgacatgac agattattga
     1201 ataaagggag ttttgagttc ttttcagccc ctaggagcat ccgttactct tgttctatgt
     1261 ttagaagtcg aaaaattact cgttttttaa agaagagtaa aggcattttc ttctaaatgt
     1321 tgttgtgttg gcatgtttca ttcgcatttt ctcatggtat caaggaaaag tgattgcaat
     1381 tagagggatc aaaaactata aaatttggaa ttgtttttgc aaaaactatg catgagactg
     1441 aacttttgga tttaagggat aaaaaactat aaaataccaa atccaaaagt tcagtctcat
     1501 ttaaaacaat taagaaccca aaataaccca tttaaagcaa caaaagaaaa aagagagtag
     1561 cccaataggc ccatgttgtt aattaaacaa cttatgcggc gcataacgga tgattctacg
     1621 atcaaaactg gaaatagatg cccataaaat tacgaagaaa atcatgtgcc taaagcatat
     1681 atgagcttta gctgcttcag gtcagagccg gctctgattg ctagcatttt aaaccaatca
     1741 cggaaatggt ccggcaaaat gtttctattt tatttaatgt atttatttta aatacggaaa
     1801 taaattttat gaaagaatcc atcaaacacg ttatagccgt gctttatagc tatttgtagc
     1861 aagagcaaat gtaccacacc acaccgtcca acaactacaa ccgttcaatt tctagttctt
     1921 ttttcttcac gtgtcacgaa aacttgaaat gaaaacgaaa tgattttcca aacaaaagag
     1981 gaaagatctt gcaaatcaga taactcgaaa cttttattta cggtgtatct tctaaacaat
     2041 cctttaaatt tcattttata atttaattac tttgcaaaca agcagattta cattttaacc
     2101 gaaaatacaa ttaatgtctt aggttcaact tcacggtgaa atatgttcca catgtttatt
     2161 gggttcaaac tcgaaccgat atttattgag cgcttaaaat gaaatattta aattctgtcc
     2221 aacgtcaaaa taatttgaga tactaacaag aaaccttcgg tagttgatgg taatcaattt
     2281 ggaaaagaat atattttatg gtgacttggg accgaagaaa attacaaaag atatgaagat
     2341 ttttattaag tttccgtgtt gtatttcgct tgctattatg tgagttcttg gttataaatg
     2401 ccgcttcttc cttcatggtt tcttacttca tcatcacgca atctcttgat tcacgatgcc
     2461 aaagaagaag agaaaggtca agggtgggcg cgccgaccca gctttcttgt acaaagtggt
     2521 tgataacagc gctggaggct ccggtgggag catggtgagc aagggcgagg agctgttcac
     2581 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     2641 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     2701 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgat
     2761 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2821 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2881 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2941 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     3001 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     3061 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     3121 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa
     3181 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     3241 cactctcggc atggacgagc tgtacaaggc tggaggctcc ggtgggagca tggtgagcaa
     3301 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     3361 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     3421 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     3481 cttcggctac ggcctgatgt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     3541 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     3601 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     3661 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     3721 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     3781 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     3841 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta
     3901 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     3961 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggctg gaggctccgg
     4021 tgggagcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     4081 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     4141 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     4201 gcccaccctc gtgaccacct tcggctacgg cctgatgtgc ttcgcccgct accccgacca
     4261 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     4321 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     4381 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     4441 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     4501 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     4561 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     4621 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     4681 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     4741 caagtaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc gagctcgaat
     4801 ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     4861 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     4921 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     4981 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     5041 tcatctatgt tactagatcg ggaattggtt ccggaaccaa ttcgtaatca tgtcatagct
     5101 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     5161 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaggct gaattaggcg
     5221 cgcctatttc tgaattcaac acattgcgga cgtttttaat gtactgaatt aacgccgaat
     5281 taattcgggg gatctggatt ttagtactgg attttggttt taggaattag aaattttatt
     5341 gatagaagta ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg
     5401 agcgaaaccc tataggaacc ctaattccct tatctgggaa ctactcacac attattatgg
     5461 agaaactcga gcttgtcgat cgacagatcc cggtcggcat ctactctatt tctttgccct
     5521 cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt
     5581 ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg
     5641 gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa
     5701 gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagtc gtggcgatcc
     5761 tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca
     5821 cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct
     5881 ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc
     5941 cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag
     6001 agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg
     6061 gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg
     6121 tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatagc
     6181 ctccgcgacc ggttgtagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac
     6241 accctgtgaa cggcgggaga tgcaataggt caggctctcg ctaaactccc caatgtcaag
     6301 cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta
     6361 gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct
     6421 gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt
     6481 ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggctttttca tatctcattg
     6541 ccccccggat ctgcgaaagc tcgagagaga tagatttgta gagagagact ggtgatttca
     6601 gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata
     6661 gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga
     6721 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     6781 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
     6841 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
     6901 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
     6961 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
     7021 gttcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
     7081 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag
     7141 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt
     7201 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt
     7261 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt
     7321 agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgaa
     7381 ttcagaaata aattcagcct aattcggcgt taattcagta cattaaaaac gtccgcaatg
     7441 tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca
     7501 gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat
     7561 cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac
     7621 cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg
     7681 gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aattcgggca
     7741 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     7801 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     7861 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     7921 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     7981 cagggcagtc gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta
     8041 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     8101 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat
     8161 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     8221 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     8281 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     8341 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     8401 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     8461 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     8521 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     8581 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     8641 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     8701 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     8761 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat
     8821 tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca
     8881 cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca
     8941 taataagccc tacacaaatt gggagatata tcatgcatga ccaaaatccc ttaacgtgag
     9001 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     9061 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     9121 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9181 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     9241 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     9301 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     9361 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     9421 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     9481 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     9541 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     9601 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     9661 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     9721 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     9781 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     9841 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     9901 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     9961 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
    10021 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
    10081 cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc
    10141 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
    10201 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
    10261 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
    10321 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
    10381 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
    10441 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
    10501 acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
    10561 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
    10621 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
    10681 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
    10741 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
    10801 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
    10861 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
    10921 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
    10981 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
    11041 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
    11101 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
    11161 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
    11221 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
    11281 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
    11341 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
    11401 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct
    11461 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
    11521 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
    11581 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
    11641 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc
    11701 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
    11761 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
    11821 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
    11881 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
    11941 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
    12001 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
    12061 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
    12121 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
    12181 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
    12241 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
    12301 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
    12361 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
    12421 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
    12481 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
    12541 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
    12601 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
    12661 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
    12721 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
    12781 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
    12841 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac
    12901 gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt
    12961 aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt
    13021 tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg
    13081 cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc
    13141 gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc
    13201 agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg
    13261 cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg
    13321 aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg
    13381 gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg
    13441 tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg
    13501 ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg
    13561 atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc
    13621 aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg
    13681 ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac
    13741 gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg
    13801 tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag
    13861 cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca
    13921 catacaaatg gacgaacgga taaacct
//
