LOCUS       sequence-377716-       14361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for plant responses to bacterial infection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14361)
  AUTHORS   Zhu J, Lolle S, Tang A, Guel B, Kvitko B, Cole B, Coaker G
  TITLE     Single-cell profiling of Arabidopsis leaves to Pseudomonas syringae 
            infection.
  JOURNAL   Cell Rep. 2023 Jun 20;42(7):112676. doi: 
            10.1016/j.celrep.2023.112676.
  PUBMED    37342910
REFERENCE   2  (bases 1 to 14361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-06-20"; volume: "42(7):112676. doi"; pages: " 
            10.1016/j.celrep.2023.112676"
COMMENT     Sequence Label: pPIP1;4-NLS-3xmCitrine
FEATURES             Location/Qualifiers
     source          1..14361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..347
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2873..2893
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(2910..2934)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2966..2968,2969..2971,2972..3682)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3485..4222
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="DGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKAGGSGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVS
                     GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMP
                     EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
                     NVYIMADKQKNGIKVNFKIRHNIE"
     CDS             join(3704..3706,3707..3709,3710..4420)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4223..4960
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="DGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKAGGSGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVS
                     GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMP
                     EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
                     NVYIMADKQKNGIKVNFKIRHNIE"
     CDS             join(4442..4444,4445..4447,4448..5158)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      5221..5473
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5506..5522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5506..5522)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5519..5541)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5530..5546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5554..5560,5561..5578,5579..5584))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5599..5620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    5705..5879
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5920..6945)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(7012..7688)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(7055..7075)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    7883..7907
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8428..9219
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9465..10053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9954..9973
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10207..10224
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10239..10379
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10305..10324)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10465..10487
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10723..10917
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10983..12056)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12485..13114)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gcaggctccg cggccgcccc
      361 cttcacccga actttactag ttactatcta ccaatcaggt tatgaaagta ggcaaaaggg
      421 gaagggtcaa ggcccatgag acatattttc caaatagaca ctaaaaatga gccagatttt
      481 tcaacaacac atttggttta agggctcaaa aaatatttgt caacacccag ttttaaaaca
      541 aaagaaaata gtaaattaca ggcccaaatg tttttgttgc cctatagata ttagtatttc
      601 ttaaccagtc gctgataatt attgatgaat agttgagaga cgataaagat tgagctgtca
      661 ggaatacaaa tcaaacatga tattgttcgt ttacattatt actcaacgaa cattcacgtt
      721 tccaaattct gatatctttt ctaatatttc tccacatgta gcatatcatt agttactgtt
      781 taaaaatgag taccaaaaac gtgaaattat tacaataacg tataaatgtt aaatatttcg
      841 agcaatcaat acttgtcaag actcattgtt agtaatgcat cactataatt cattcttttt
      901 ttgtcttggt caaacatgta tgaagcgagc agagtctatg aatgtcatat tatagacata
      961 cgtatgcggc tttggtcggg accggcccaa taaaccaata cacatgttca acgggttctt
     1021 ccacgtgtca aacttatgtt ggctggacgg tatgtgactg tgcattgtgg ttacgtttcc
     1081 aaaatgaata cgtaagaact tttcctaaat tatgtctttt gcacaatact gtaagtctgt
     1141 aactatggta ctaaacatta taatatcgaa ttggaaacat ttacatttga gaattttagt
     1201 gtttgttggc aacgaagcat gtgagatcac agagaagcaa tactcatctc tgagctgttc
     1261 aaacagtttt acatgtcagc acccaaatat agtcactata ttcaaaaaca aaattattta
     1321 cttgcattca atgctagcct tttttattcg gtccacaaca aattataaat atttgtatat
     1381 aagaaaaatc agagacctga ttttgtaatg cgtggcttgt caatttcaag caacacagaa
     1441 aaaagctaac taagcaaaaa acaaaaaaaa ataacaagtg gctggttgat agaatgtatc
     1501 tttcatcagt acataacact gtttttttgt caacgtaaaa cagattttta taacttttgt
     1561 ttgccctcaa cttttttcat tacattttct ggaatatcgc atggttgaat aattaaaagt
     1621 taaaaatgat ttttatgaag cacggcatta tactatatag tcagtcccaa cgaaaatgac
     1681 tttacataaa gctgtcaatt ttttctccaa cggtcagata attgtttcgt tgaatagtca
     1741 gttttttttt ttactatttt actattgggc tttattgggc tcttacaatt ttatcgattt
     1801 ttgtttaccg tccagaaagt ggaccctgtg ggaagaggaa aagaaaaaaa aagggtcagg
     1861 ttttttgtct ttatacgaga gcacatcggt acgttccaga acaaccctct ctagtctcct
     1921 ccatcattat ctttatttaa tagtaaccct agtacaaatt accaaactat tcaacattaa
     1981 cataattatt caacattcac catcatgaat aataactagt tgagaaaaac gtaactagac
     2041 tagtttgatc tgtcaacata aaaagagaaa aaagaatttc gtgactagta aagtttaaga
     2101 ccattcacaa atgatcattg aaattattag ttctaaactc ttagtttggt tatctatttg
     2161 gttttgattc gtatctattt ggttattcgg ttttttggtt ttaaagttta gtattcattc
     2221 gactattttg aaattttggt tcgttttttt tttttttttt tgaaatttaa tatccattcg
     2281 attattttgc ttttaatatt tttaaatatt tcaattattt tttgatattt tttgaatatt
     2341 gttactattt aatatatatt tttaaatatt tttaattact aagtatagaa ataactaata
     2401 tttttgaaaa taaaattata ttttggatat tttagatcat ctaaaatatt ttagttaggt
     2461 actaattcat ttttggttat tttgattata aaatttaata tctattcaac aatttttaaa
     2521 ttgttgattc aattcggttc tgtttttttt tttcatttag gtttagtaca tttggttcag
     2581 tttatttgac tctcagttta tttgactaag ccttgaaatt aggtgttaac tattggttat
     2641 tctagttgaa atttgaaata aagaaaaaac tatacgttat actattttat tttcttagca
     2701 ataactaaaa ataaatgttt ggaaacgtta ggcaatttta ttatattcta tcgttgttct
     2761 ctataaatga agtctcgacc acacacttgg tagctacaca cgagaaagaa aaagctcaca
     2821 actttaacct ttctggagag agaagaacaa aagagggaga gggagagaaa tgccaaagaa
     2881 gaagagaaag gtcaagggtg ggcgcgccga cccagctttc ttgtacaaag tggttgataa
     2941 cagcgctgga ggctccggtg ggagcatggt gagcaagggc gaggagctgt tcaccggggt
     3001 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     3061 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     3121 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgatgtgctt
     3181 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3241 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3301 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3361 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     3421 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     3481 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3541 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     3601 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     3661 cggcatggac gagctgtaca aggctggagg ctccggtggg agcatggtga gcaagggcga
     3721 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     3781 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     3841 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg
     3901 ctacggcctg atgtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     3961 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     4021 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     4081 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     4141 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     4201 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     4261 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     4321 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     4381 cgccgccggg atcactctcg gcatggacga gctgtacaag gctggaggct ccggtgggag
     4441 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     4501 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     4561 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     4621 cctcgtgacc accttcggct acggcctgat gtgcttcgcc cgctaccccg accacatgaa
     4681 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     4741 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     4801 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     4861 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     4921 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     4981 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     5041 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     5101 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     5161 agcttgcatg cctgcaggtc gactctagag gatccccggg taccgagctc gaatttcccc
     5221 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     5281 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     5341 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     5401 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     5461 atgttactag atcgggaatt ggttccggaa ccaattcgta atcatgtcat agctgtttcc
     5521 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     5581 taaagcctgg ggtgcctaat gagtgagcta actcacatta ggctgaatta ggcgcgccta
     5641 tttctgaatt caacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc
     5701 gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga
     5761 agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa
     5821 accctatagg aaccctaatt cccttatctg ggaactactc acacattatt atggagaaac
     5881 tcgagcttgt cgatcgacag atcccggtcg gcatctactc tatttctttg ccctcggacg
     5941 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
     6001 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
     6061 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
     6121 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agtcgtggcg atcctgcaag
     6181 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
     6241 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
     6301 aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg
     6361 cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg
     6421 cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc
     6481 agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
     6541 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tagcctccgc
     6601 gaccggttgt agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg
     6661 tgaacggcgg gagatgcaat aggtcaggct ctcgctaaac tccccaatgt caagcacttc
     6721 cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc
     6781 atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc
     6841 acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat
     6901 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc attgcccccc
     6961 ggatctgcga aagctcgaga gagatagatt tgtagagaga gactggtgat ttcagcgtgt
     7021 cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga
     7081 ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt
     7141 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
     7201 actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta
     7261 ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa
     7321 tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
     7381 tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttcac
     7441 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
     7501 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
     7561 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
     7621 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
     7681 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
     7741 gagtgtcgtg ctccaccatg ttggcaagct gctctagcca atacgcaaac cgaattcaga
     7801 aataaattca gcctaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat
     7861 taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac
     7921 agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc
     7981 gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc
     8041 tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt
     8101 agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc
     8161 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     8221 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     8281 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     8341 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     8401 agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga agtatcgact
     8461 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     8521 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     8581 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     8641 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     8701 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     8761 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     8821 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     8881 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     8941 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     9001 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     9061 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     9121 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     9181 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag aaattcgttc
     9241 aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat
     9301 tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa
     9361 gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg
     9421 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9481 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     9541 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     9601 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9661 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9721 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9781 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9841 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9901 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9961 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10021 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10081 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10141 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    10201 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
    10261 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
    10321 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
    10381 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
    10441 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
    10501 caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc
    10561 gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca
    10621 gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta
    10681 ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg
    10741 gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct
    10801 tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg
    10861 tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga
    10921 aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta
    10981 cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc
    11041 gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc
    11101 agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag
    11161 atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag
    11221 agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc
    11281 gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac
    11341 tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg
    11401 gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg
    11461 gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc
    11521 cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc
    11581 ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg
    11641 tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct
    11701 ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg
    11761 gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa
    11821 aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc
    11881 ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt
    11941 gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg
    12001 tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg
    12061 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta
    12121 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
    12181 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
    12241 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
    12301 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
    12361 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
    12421 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
    12481 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
    12541 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
    12601 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
    12661 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
    12721 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
    12781 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
    12841 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
    12901 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
    12961 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
    13021 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
    13081 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
    13141 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
    13201 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
    13261 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
    13321 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
    13381 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
    13441 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc
    13501 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga
    13561 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg
    13621 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg
    13681 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg
    13741 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac
    13801 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt
    13861 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg
    13921 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc
    13981 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc
    14041 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg
    14101 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca
    14161 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac
    14221 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg
    14281 aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca
    14341 aatggacgaa cggataaacc t
//
