LOCUS       sequence-377717-       10061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the constitutive expression of Cup2:Gal4AD and 
            copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio
            S, Orzaez D, Patron N
  TITLE     Tunable control of insect pheromone biosynthesis in Nicotiana 
            benthamiana.
  JOURNAL   Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048.
  PUBMED    37032497
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048."
COMMENT     Sequence Label: GB4068
FEATURES             Location/Qualifiers
     source          1..10061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             254..274
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(275..298,299..301,302..2785,2786..2791,2792..2857,
                     2858..2860,2861..4375)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(275..298,299..301,302..2788,2789..2791,2792..4375)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             275..4375
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4394..4414
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4418..4438
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4442..4462
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4561..4813
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5262..6053)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(6275..6937)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      6938..7709
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     misc_feature    7929..7953
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    7980..8004
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(8284..8536)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(9527..9706)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 gcgatgcgtc ttttccgctg aaccgttcca gcaaaaaaga ctaggagagc gatgcgtctt
       61 ttccgctgaa ccgttccagc aaaaaagact atcccgtcct aatacctcta cgtgcaagca
      121 gtataaaaac aaaatacata aacataacta aaatctcata tcgttgtaat tgtcatatac
      181 tagtggtaat taagtccagt tacacttaag gttaaatttg ctgactcttt tttacctttc
      241 atttctgaaa atgcccaaga agaagaggaa ggtggacaag aagtactcca ttgggctcgc
      301 tatcggcaca aacagcgtcg gctgggccgt cattacggac gagtacaagg tgccgagcaa
      361 aaaattcaaa gttctgggca ataccgatcg ccacagcata aagaagaacc tcattggcgc
      421 cctcctgttc gactccgggg aaacggccga agccacgcgg ctcaaaagaa cagcacggcg
      481 cagatatacc cgcagaaaga atcggatctg ctacctgcag gagatcttta gtaatgagat
      541 ggctaaggtg gatgactctt tcttccatag gctggaggag tcctttttgg tggaggagga
      601 taaaaagcac gagcgccacc caatctttgg caatatcgtg gacgaggtgg cgtaccatga
      661 aaagtaccca accatatatc atctgaggaa gaagcttgta gacagtactg ataaggctga
      721 cttgcggttg atctatctcg cgctggcgca tatgatcaaa tttcggggac acttcctcat
      781 cgagggggac ctgaacccag acaacagcga tgtcgacaaa ctctttatcc aactggttca
      841 gacttacaat cagcttttcg aagagaaccc gatcaacgca tccggagttg acgccaaagc
      901 aatcctgagc gctaggctgt ccaaatcccg gcggctcgaa aacctcatcg cacagctccc
      961 tggggagaag aagaacggcc tgtttggtaa tcttatcgcc ctgtcactcg ggctgacccc
     1021 caactttaaa tctaacttcg acctggccga agatgccaag cttcaactga gcaaagacac
     1081 ctacgatgat gatctcgaca atctgctggc ccagatcggc gaccagtacg cagacctttt
     1141 tttggcggca aagaacctgt cagacgccat tctgctgagt gatattctgc gagtgaacac
     1201 ggagatcacc aaagctccgc tgagcgctag tatgatcaag cgctatgatg agcaccacca
     1261 agacttgact ttgctgaagg cccttgtcag acagcaactg cctgagaagt acaaggaaat
     1321 tttcttcgat cagtctaaaa atggctacgc cggatacatt gacggcggag caagccagga
     1381 ggaattttac aaatttatta agcccatctt ggaaaaaatg gacggcaccg aggagctgct
     1441 ggtaaagctt aacagagaag atctgttgcg caaacagcgc actttcgaca atggaagcat
     1501 cccccaccag attcacctgg gcgaactgca cgctatcctc aggcggcaag aggatttcta
     1561 cccctttttg aaagataaca gggaaaagat tgagaaaatc ctcacatttc ggatacccta
     1621 ctatgtaggc cccctcgccc ggggaaattc cagattcgcg tggatgactc gcaaatcaga
     1681 agagactatc actccctgga acttcgagga agtcgtggat aagggggcct ctgcccagtc
     1741 cttcatcgaa aggatgacta actttgataa aaatctgcct aacgaaaagg tgcttcctaa
     1801 acactctctg ctgtacgagt acttcacagt ttataacgag ctcaccaagg tcaaatacgt
     1861 cacagaaggg atgagaaagc cagcattcct gtctggagag cagaagaaag ctatcgtgga
     1921 cctcctcttc aagacgaacc ggaaagttac cgtgaaacag ctcaaagaag attatttcaa
     1981 aaagattgaa tgtttcgact ctgttgaaat cagcggagtg gaggatcgct tcaacgcatc
     2041 cctgggaacg tatcacgatc tcctgaaaat cattaaagac aaggacttcc tggacaatga
     2101 ggagaacgag gacattcttg aggacattgt cctcaccctt acgttgtttg aagataggga
     2161 gatgattgaa gaacgcttga aaacttacgc tcatctcttc gacgacaaag tcatgaaaca
     2221 gctcaagagg cgccgatata caggatgggg gcggctgtca agaaaactga tcaatgggat
     2281 ccgagacaag cagagtggaa agacaatcct ggattttctt aagtccgatg gatttgccaa
     2341 ccggaacttc atgcagttga tccatgatga ctctctcacc tttaaggagg acatccagaa
     2401 agcacaagtt tctggccagg gggacagtct ccacgagcac atcgctaatc ttgcaggtag
     2461 cccagctatc aaaaagggaa tactgcagac cgttaaggtc gtggatgaac tcgtcaaagt
     2521 aatgggaagg cataagcccg agaatatcgt tatcgagatg gcccgagaga accaaactac
     2581 ccagaaggga cagaagaaca gtagggaaag gatgaagagg attgaagagg gtataaaaga
     2641 actggggtcc caaatcctta aggaacaccc agttgaaaac acccagcttc agaatgagaa
     2701 gctctacctg tactacctgc agaacggcag ggacatgtac gtggatcagg aactggacat
     2761 caatcggctc tccgactacg acgtggatgc catcgtgccc cagtcttttc tcaaagatga
     2821 ttctattgat aataaagtgt tgacaagatc cgataaaaat agagggaaga gtgataacgt
     2881 cccctcagaa gaagttgtca agaaaatgaa aaattattgg cggcagctgc tgaacgccaa
     2941 actgatcaca caacggaagt tcgataatct gactaaggct gaacgaggtg gcctgtctga
     3001 gttggataaa gccggcttca tcaaaaggca gcttgttgag acacgccaga tcaccaagca
     3061 cgtggcccaa attctcgatt cacgcatgaa caccaagtac gatgaaaatg acaaactgat
     3121 tcgagaggtg aaagttatta ctctgaagtc taagctggtt tcagatttca gaaaggactt
     3181 tcagttttat aaggtgagag agatcaacaa ttaccaccat gcgcatgatg cctacctgaa
     3241 tgcagtggta ggcactgcac ttatcaaaaa atatcccaag cttgaatctg aatttgttta
     3301 cggagactat aaagtgtacg atgttaggaa aatgatcgca aagtctgagc aggaaatagg
     3361 caaggccacc gctaagtact tcttttacag caatattatg aattttttca agaccgagat
     3421 tacactggcc aatggagaga ttcggaagcg accacttatc gaaacaaacg gagaaacagg
     3481 agaaatcgtg tgggacaagg gtagggattt cgcgacagtc cggaaggtcc tgtccatgcc
     3541 gcaggtgaac atcgttaaaa agaccgaagt acagaccgga ggcttctcca aggaaagtat
     3601 cctcccgaaa aggaacagcg acaagctgat cgcacgcaaa aaagattggg accccaagaa
     3661 atacggcgga ttcgattctc ctacagtcgc ttacagtgta ctggttgtgg ccaaagtgga
     3721 gaaagggaag tctaaaaaac tcaaaagcgt caaggaactg ctgggcatca caatcatgga
     3781 gcgatcaagc ttcgaaaaaa accccatcga ctttctcgag gcgaaaggat ataaagaggt
     3841 caaaaaagac ctcatcatta agcttcccaa gtactctctc tttgagcttg aaaacggccg
     3901 gaaacgaatg ctcgctagtg cgggcgagct gcagaaaggt aacgagctgg cactgccctc
     3961 taaatacgtt aatttcttgt atctggccag ccactatgaa aagctcaaag gatctcccga
     4021 agataatgag cagaagcagc tgttcgtgga acaacacaaa cactaccttg atgagatcat
     4081 cgagcaaata agcgaattct ccaaaagagt gatcctcgcc gacgctaacc tcgataaggt
     4141 gctttctgct tacaataagc acagggataa gcccatcagg gagcaggcag aaaacattat
     4201 ccacttgttt actctgacca acttgggcgc gcctgcagcc ttcaagtact tcgacaccac
     4261 catagacaga aagcggtaca cctctacaaa ggaggtcctg gacgccacac tgattcatca
     4321 gtcaattacg gggctctatg aaacaagaat cgacctctct cagctcggtg gagacagcag
     4381 ggctgattcg gatcctaaaa agaagcgaaa ggtagacccg aagaaaaaga ggaaggtaga
     4441 tccaaagaag aagcgcaagg tagaagtgtt tgaatttgaa tatctggatg ataaagtgct
     4501 ggaagaactg ctggatagcg aagaacgcaa acgctgagct tggaatggat cttcgatccc
     4561 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     4621 acgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     4681 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     4741 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     4801 atgttactag atcgggaatt gccaagctaa ttcttgaaga cgaaagggcc tcgtgatacg
     4861 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     4921 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4981 tccgctcatg agacaataac cctgataaat gcttcaataa tgggaccgac tcgcgctgtc
     5041 aggagaccgg acaaggatgc gagctagcct gcaggaattg ttgattttgt gatgactgat
     5101 ggcaggatat atgcggttgt aattcatttt tattgtctaa atttctgtat ttgtttgttt
     5161 gttcggttgt aaattttttt ggaagaacaa gaaaagaaaa aacacccgtt agggtgtttt
     5221 tagttagtgt ggcgcgccga cttgcgacat gcggtccttt gttatttgcc gactaccttg
     5281 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     5341 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     5401 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     5461 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     5521 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     5581 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     5641 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     5701 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     5761 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     5821 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     5881 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
     5941 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
     6001 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct catgatgttt
     6061 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     6121 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca
     6181 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     6241 gtcaaggttc gatcggattg gcggttatgc ggttctaccg gcgcggcagc gttacccgtg
     6301 tcggcggctc caacggctcg ccatcgtcca gaaaacacgg ctcatcgggc atcggcaggc
     6361 gctgctgccc gcgccgttcc cattcctccg tttcggtcaa ggctggcagg tctggttcca
     6421 tgcccggaat gccgggctgg ctgggcggct cctcgccggg gccggtcggt agttgctgct
     6481 cgcccggata cagggtcggg atgcggcgca ggtcgccatg ccccaacagc gattcgtcct
     6541 ggtcgtcgtg atcaaccacc acggcggcac tgaacaccga caggcgcaac tggtcgcggg
     6601 gctggcccca cgccacgcgg tcattgacca cgtaggccga cacggtgccg gggccgttga
     6661 gcttcacgac ggagatccag cgctcggcca ccaagtcctt gactgcgtat tggaccgtcc
     6721 gcaaagaacg tccgatgagc ttggaaagtg tcttctggct gaccaccacg gcgttctggt
     6781 ggcccatctg cgccacgagg tgatgcagca gcattgccgc cgtgggtttc ctcgcaataa
     6841 gcccggccca cgcctcatgc gctttgcgtt ccgtttgcac ccagtgaccg ggcttgttct
     6901 tggcttgaat gccgatttct ctggactgcg tggccatgct tatctccatg cggtaggggt
     6961 gccgcacggt tgcggcacca tgcgcaatca gctgcaactt ttcggcagcg cgacaacaat
     7021 tatgcgttgc gtaaaagtgg cagtcaatta cagattttct ttaacctacg caatgagcta
     7081 ttgcgggggg tgccgcaatg agctgttgcg tacccccctt ttttaagttg ttgattttta
     7141 agtctttcgc atttcgccct atatctagtt ctttggtgcc caaagaaggg cacccctgcg
     7201 gggttccccc acgccttcgg cgcggctccc cctccggcaa aaagtggccc ctccggggct
     7261 tgttgatcga ctgcgcggcc ttcggccttg cccaaggtgg cgctgccccc ttggaacccc
     7321 cgcactcgcc gccgtgaggc tcggggggca ggcgggcggg cttcgccctt cgactgcccc
     7381 cactcgcata ggcttgggtc gttccaggcg cgtcaaggcc aagccgctgc gcggtcgctg
     7441 cgcgagcctt gacccgcctt ccacttggtg tccaaccggc aagcgaagcg cgcaggccgc
     7501 aggccggagg cttttcccca gagaaaatta aaaaaattga tggggcaagg ccgcaggccg
     7561 cgcagttgga gccggtgggt atgtggtcga aggctgggta gccggtgggc aatccctgtg
     7621 gtcaagctcg tgggcaggcg cagcctgtcc atcagcttgt ccagcagggt tgtccacggg
     7681 ccgagcgaag cgagccagcc ggtggccgct cgcggccatc gtccacatat ccacgggctg
     7741 gcaagggagc gcagcgaccg cgcagggcga agcccggaga gcaagcccgt agggggccat
     7801 catcagttcg gtggtcttcc gacgaacaat aaggccgcaa atcgcggcct tttttattga
     7861 taacaaaacc ggctcagttc tgcgtagaaa ccaacatgca agctccaccg ggtgcaaagc
     7921 ggcagcggcg gcaggatata ttcaattgta aatggcttca tgtccgggaa atctactggt
     7981 ggcaggatat attgtggtgt aaacaatgga gaaaaagatt aattaagtac tagtagcctg
     8041 gtcgaaacgg tctcaggaga gcgcgagtcg gtcccattat tgaagcattt atcagggtta
     8101 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8161 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     8221 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattag cttggcaatt
     8281 cccgatctag taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata
     8341 ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc
     8401 tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa
     8461 attatatgat aatcgtcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc
     8521 aaatgtttga acgatcggga tcgaagatcc attccaagct cactcttttt ttgggtttgg
     8581 tggggtatct tcatcatcga atagatagtt atatacatca tccattgtag tggtattaaa
     8641 catccctgta gtgattccaa acgcgttata cgcagtttgg tccgtccaac caggtgacag
     8701 tggttttgaa ttattaccat catcaatttt actagccgtg atttcattat tcatgaagtt
     8761 atcatgaacg ttagaggagg caattggttg tgaaagcgct tgagaatttg ttggctgtga
     8821 atgtgagtta tgcgaagata cttgtttgta tagctcgttc aactcattga tggagaatgg
     8881 aatgtcaata tcgtttagtg ctgtgttcgt agttgtatat gcgcttcctg tttcattgat
     8941 actaacaggt tccatagata tactagggct ttgctcaagc ggatcgaggg aagccaaatc
     9001 aagattgtca atgctgtcgg ccttaatttc tccgttttgc agaggtggct tcaaactcgg
     9061 caatgtggtt gtcatgtcta catccgacga gcgaccattt aggcccaaaa gaacgtccag
     9121 atctgcaata ccaagaccat tgacatgcgc tgcttcatca tttgcccttc tgtggcatga
     9181 ccctcccttt gatttccggc tgcttttcct cttggtatga catttacaag gttcaccctc
     9241 atcacaacga catcgtgttt catcttcctt gctgccaaca gctggccgtc gtgcagaggc
     9301 acacatgcac ccaccggatg ggttgaagtt cttggttctt ctcagctctt tacaatggcc
     9361 acatgtggtc gatggtcttc ccttgcgtct gatcatctgt agcggaccat cagtgtgagt
     9421 acactgcgcc gccctgtgac ccctgataca cgtttcacag gcatatttga ccccgttaat
     9481 tacgaccatc attgcctcga tcgagttgag agtgaatatg agactctaat tggataccga
     9541 ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct agctgatagt
     9601 gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct tagctcatta
     9661 aactccagaa acccgcggct cagtggctcc ttcaacgttg cggttctgtc agttccaaac
     9721 gtaaaacggc ttgtcccgcg tcatcggcgg gggtcatacc gtgactccct taattctcat
     9781 gtatctcctg acagcgaaat gattgatgaa gaacaatggt ggatgaagaa caaagaagga
     9841 gggagctttt gttcaagatg aacaaagaac aatagtggat gaagaacaaa gtgaaaaaaa
     9901 taaaaaaaaa tgtatggtta aataaagagt aaagttacca ttgagactcc tgacggagag
     9961 tggtcttggg agagcgatgc gtcttttccg atgaaccgtt ccagcaaaaa agactaagcg
    10021 atgcgtcttt tccgctgaac cgttccagca aaaaagacta a
//
