LOCUS       sequence-377722-       10171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Salmon Alphavirus polyprotein with mEGFP N-terminal 
            fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Teige LH, Kumar S, Johansen GM, Wessel O, Vendramin N, Lund M, 
            Rimstad E, Boysen P, Dahle MK
  TITLE     Detection of Salmonid IgM Specific to the Piscine Orthoreovirus 
            Outer Capsid Spike Protein Sigma 1 Using Lipid-Modified Antigens in 
            a Bead-Based Antibody Detection Assay.
  JOURNAL   Front Immunol. 2019 Sep 6;10:2119. doi: 10.3389/fimmu.2019.02119. 
            eCollection 2019.
  PUBMED    31552049
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Immunol."; date: "2019-09-6"; volume: "10:2119. doi"; pages: " 
            10.3389/fimmu.2019.02119. eCollection 2019"
COMMENT     Sequence Label: pcDNA3.1-Hyg-mEGFP-pp4640
FEATURES             Location/Qualifiers
     source          1..10171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(901..903,904..906,907..1617)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLK
                     ERVTAAGITLGMDELYK"
     primer_bind     complement(946..967)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1207..1226)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(5593..5610)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5599..5823
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5869..6297
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5956..5975)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6166..6187
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6306..6326)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6311..6640
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6491..6626
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6553..6572
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6689..7714
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6689..7714
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7844..7965
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7881..7900)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7935..7954
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8014..8030)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8014..8030)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8027..8049)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8038..8054
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8062..8068,8069..8086,8087..8092))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8107..8128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8245..8262)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8416..9004)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8496..8515)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9175..9966,9967..10035))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9798..9817
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10036..10140)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      961 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     1021 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1081 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     1141 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     1201 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1261 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1321 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     1381 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     1441 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1501 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1561 ctgaaggagc gcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagctc
     1621 gagatgtttc ccatgcaatt caccaactca gcctatcgcc agatggagcc catgtttgca
     1681 ccgggttccc gaggacaagt acagccgtac cggccgcgca ctaagcgccg ccaggagccg
     1741 caagtcggca acgccgccat tactgccctc gcgaaccaga tgagtgcgct ccagttgcag
     1801 gtagctggac ttgccggcca ggcaagggtg gaccgccgtg ggccaagacg tgttcagaag
     1861 aacaagcaga agaagaagaa ctcttccaac ggagaaaaac ccaaagagaa gaagaagaag
     1921 caaaaacaac aggagaagaa gggaagcggt ggcgaaaaag tcaagaagac taggaaccga
     1981 cccgggaagg aggtaaggat ctccgtaaag agggcccgac agagcacctt ccccgtgtac
     2041 cacgaaggtg ctatatccgg ctacgctgtg ctgattggat ctcgcgtatt caagccggca
     2101 cacgtgaagg gtaagatcga ccaccctgaa ctggcagaca tcaagttcca ggtcgccgag
     2161 gacatggacc tcgaagcagc tgcgtacccg aagagcatgc gagaccaagc ggctgaacca
     2221 gcgaccatga tggacggcgt gtacaactgg gagtatggca ctatcagagt ggaggataat
     2281 gtcataatcg acgcaagcgg taggggcaag ccgggtgaca gtggcagggc catcaccgac
     2341 aactcgggaa aggttgttgg tattgtcctc ggaggaggac ccgatggcag gcgcacacgc
     2401 ctctccgtga taggtttcga caagaagatg aaggctaggg agatcgccta cagtgatgcc
     2461 ataccttgga cacgcgctcc ggccctcctg ctgctgccta tggttattgc ctgcacctac
     2521 aattccaaca ccttcgattg ctccaaaccg tcctgccagg actgctgcat tactgctgaa
     2581 ccaaagaagg ccatgaccat gctgaaggac aatctgaacg acccgaacta ctgggaccta
     2641 ctcattgctg tcaccacctg tggctccgcc cggagaaaga gggctgtgtc ttacgacaca
     2701 cagatcctcg ccgcccacgc agctgcctcc ccatacaggg cgtactgccc cgattgtgac
     2761 ggaacagcgt gtatctcgcc gatagccatc gacgaggtgg tgagcagtgg cagcgaccac
     2821 gtcctccgca tccgggttgg ttctcaatcg ggagtgaccg ctaagggtgg tgcggcgggt
     2881 gaaacctctc tgcgatacct gggaagggac gggaaggttc acgccgcaga caacacgcga
     2941 ctcgtggtgc gcacgactgc aaagtgcgac gtgctgcagg ccactggcca ctacatcctg
     3001 gccaactgcc cagtggggca gagcctaacc gttgcggcca cactggatgg cacccggcat
     3061 caatgcacca cggttttcga acaccaagta acggagaagt tcaccagaga acgcagcaag
     3121 ggccaccatc tgtccgacat gaccaagaaa tgcaccagat tttccactac accaaaaaag
     3181 tccgccctct acctcgttga tgtgtatgac gctctgccga tttctgtaga gattagcacc
     3241 gtcgtaacat gcaacgacag ccagtgcaca gtgagggtgc cacctggtac cacagtgaaa
     3301 ttcgacaaga aatgcaagag cgctgacctg gcaaccgtca ctttcaccag cgactcccag
     3361 acgtttacgt gtgaggagcc agtcctaacg gctgccagta tcacccaggg caagccacac
     3421 ctcagatcgg caatgttgcc tagcggaggc aaggaagtga aagcaaggat cccgttcccg
     3481 ttcccgccgg aaaccgcaac ttgcagagtg agtgtagccc cactgccgtc gatcacctac
     3541 gaggaaagcg atgtcctgct ggccggtacc gcaaaatacc ctgtgctgct aaccacacgg
     3601 aaccttggtt tccatagcaa cgccacatcc gaatggatcc agggcaagta cctgcgccgc
     3661 atcccggtca cgcctcaagg gatcgagcta acatggggaa acaacgcgcc gatgcacttt
     3721 tggtcatccg tcaggtacgc atccggggac gctgatgcgt acccctggga acttctggtg
     3781 caccacatca agcaccatcc agagtacgcc tgggcgtttg taggagttgc atgcggcctg
     3841 ctggctatcg cagcgtgcat gtttgcgtgc gcatgcagca gggtgcggta ctctctggtc
     3901 gccaacacgt tcaaccccaa cccaccacca ttgaccgcac tgactgcagc actgtgttgc
     3961 ataccagggg ctcgcgcgga ccaaccctac ttggacatca ttgcctactt gtggaccaac
     4021 agcaaagtgg ccttcgggct acaatttgcg gcgcccgtgg cctgtgtgct catcattaca
     4081 tacgccctta ggcactgcag attgtgctgc aagtcttttt taggggtaag agggtggtca
     4141 gccctgctgg tcatccttgc gtatgtacag agctgcaaga gctacgaaca caccgtggtg
     4201 gtcccaatgg atccaagagc cccgtcgtac gaagcagtga taaaccggaa tgggtatgat
     4261 ccattgaagc tgaccatctc agtgaatttc accgtcatct caccaactac ggctctggaa
     4321 tattggacct gcgcaggagt ccccatcgtc gagccgcccc atgtgggctg ctgcacgtcg
     4381 gtgtcctgcc cctctgacct ctctacgctg catgcgttta ctggcaaagc tgtctccgac
     4441 gtgcactgcg atgtgcacac aaacgtgtac cccttgttgt ggggcgcggc tcactgcttc
     4501 tgttccaccg agaatacaca ggtcagcgct gtggcagcca ccgtttctga gttctgtgcc
     4561 caggactcag agcgtgccga agcgttcagc gtacacagca gctcagtcac cgctgaggtc
     4621 ctggtgacgc ttggtgaagt ggtgacggca gtccacgttt acgtggacgg ggtaacatca
     4681 gccaggggca ctgacctcaa gatcgtggct ggaccaataa caaccgacta ctccccattc
     4741 gatcgcaaag tagtccgcat cggcgaagag gtctataact atgactggcc tccttacggg
     4801 gctggccgac caggcacatt cggagacatt caagctaggt caaccaacta tgtcaaaccc
     4861 aacgatctgt atggggacat cggaattgaa gtactgcagc cgactaacga ccacgtacat
     4921 gtggcttaca cgtatacgac ctctgggtta ctgcgttggc tgcaggacgc tccgaaacca
     4981 ctcagtgtca cagcaccgca cggttgtaag atcagtgcca atccgctcct ggccctcgat
     5041 tgtggggttg gtgccgtccc catgtccatc aacattccgg acgcgaagtt tacccgcaaa
     5101 ttaaaggatc cgaaaccatc ggccctgaaa tgcgtggtgg acagctgcga gtacggggtg
     5161 gactacgggg gcgccgccac gatcacctac gagggccacg aggccgggaa gtgcgggatt
     5221 cattccctga caccaggagt ccccctgaga acatcggtgg ttgaagtggt tgctggcgcc
     5281 aataccgtca aaacgacctt ctcctcaccc acgcccgagg ttgcactgga ggtagagatc
     5341 tgttcggcaa tagtgaagtg cgctggtgag tgcactccac cgaaggaaca tgtggtcgca
     5401 accaggcctc gccatggcag cgactccgga ggctacatct ccgggcccgc aatgcgctgg
     5461 gccggaggga ttgtagggac cctagtggtc ctgttcctta tccttgccgt catctactgc
     5521 gtggtgaaga agtgccgctc caaaagaatc cggatagtca agagctaagc ttaagtttaa
     5581 accgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     5641 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     5701 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     5761 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     5821 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     5881 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5941 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6001 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6061 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     6121 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     6181 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     6241 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     6301 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     6361 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     6421 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     6481 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6541 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     6601 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     6661 atccattttc ggatctgatc agcacgtgat gaaaaagcct gaactcaccg cgacgtctgt
     6721 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
     6781 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
     6841 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
     6901 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
     6961 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
     7021 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
     7081 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
     7141 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     7201 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     7261 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
     7321 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
     7381 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
     7441 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
     7501 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
     7561 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
     7621 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
     7681 acgccccagc actcgtccga gggcaaagga atagcacgtg ctacgagatt tcgattccac
     7741 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     7801 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     7861 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7921 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     7981 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8041 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8101 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8161 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8221 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8281 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8341 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8401 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8461 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8521 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8581 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8641 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8701 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8761 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8821 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     8881 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8941 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9001 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9061 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9121 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9181 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9241 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9301 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9361 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9421 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9481 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9541 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9601 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9661 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9721 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9781 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9841 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9901 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9961 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10021 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10081 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10141 cacatttccc cgaaaagtgc cacctgacgt c
//
