LOCUS       sequence-377724-        3443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Kobayashi T, Ooms LS, Ikizler M, Chappell JD, Dermody TS
  TITLE     An improved reverse genetics system for mammalian orthoreoviruses.
  JOURNAL   Virology. 2010 Mar 15;398(2):194-200. Epub 2009 Dec 29.
  PUBMED    20042210
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2010-03-15"; volume: "398(2)"; pages: "194-200. Epub 2009 Dec
            29"
COMMENT     Sequence Label: pT7-L1-S1T3D
FEATURES             Location/Qualifiers
     source          1..3443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(147..164)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(318..906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(398..417)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1077..1868,1869..1937))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1700..1719
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1938..2042)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtccaaactg agtcagtatc atggatgaca ttgtcgtgga agtggaacgt gtagctatag
       61 tgagtcgtat tacccatccc cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      121 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      181 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      241 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      301 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      361 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      421 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      481 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      541 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      601 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      661 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      721 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
      781 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      841 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      901 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
      961 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1021 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1081 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1141 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1201 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1261 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1321 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1381 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1441 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1501 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1561 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1621 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1681 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     1741 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     1801 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     1861 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     1921 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     1981 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2041 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     2101 acctgtcacg tattgagatc tcataatcgc ttgaaacaat ggatcccacg tacgtggttg
     2161 agccgccatt ctcgccgctg ccaagtacca tgttctatat aagtaacgcg cgatggaact
     2221 tgttgtatct tttagaattc tgatttcacc tattgggcct gtccagtctt tctggggaat
     2281 gggaacctta atctccggac tctgcgtata ttcttgcaat accgtttcgg tgggtgttct
     2341 taccaagaaa ataccgctag tacatgccat agcgtcacga atgtacctca ctgaagcaaa
     2401 gtcatgttta acatacgtct ttgtccgttt acgaagctca ccgacgactg agctcagtcc
     2461 atatttctcg ccaagttccc gcgctttctt atcattggac ggcgccgacc tagcatactt
     2521 agcggacaat ggcatgactc ccatgtacat gaacttatag tctgatctag gctctaacca
     2581 ttcaagatta ggacagtatc ccccttctag agattcatac atactgtgca acgtgttttg
     2641 aggaaaaata gacttagtct ttaagataaa ccatgcataa cttgagctag ctgctgcatg
     2701 aaggcatatc tgtactccca atttcactaa tgatggatct aaacccgcag tttgttgcaa
     2761 aatagctaaa ttaacaagat cggatagtag cggagggatt gcagacgtca ttcgtggtga
     2821 cattaaccag aaaacccctg cagataagta aagataaggt gcccgatctg gtaatggacc
     2881 agatatgtga cgacagatct ctctaaaaca ccataaaggg caattatgcg atagatctgg
     2941 aatctcatca tacttctgca aaactggatc atataaagac ccccctgctt cggccactaa
     3001 tagtgccaac acaaacttct ccaatggagc cttaatctgt cgagcttgag attcagcgat
     3061 tctaccatac gttgtggcga ttctggcttg gctgtcccca tccttggcac gattctcgat
     3121 ggcctcggac aaccgcccat aagatgtcag agtctcatgt ggctccagca attcaacaat
     3181 agaacaatta ggtacgtaca cgtcatcagg gccctgatat gttttgcgac gcacacggtt
     3241 tagagcatca atgtaataat atgaatcgtg agatggtttc gtatatatag ttggagggat
     3301 tggaagcatc gcatcatcag agaaaggtat ttcatccagc gccttgtaga catcgctgcg
     3361 agtaaacata gagaatgctt ttgctgcatc ttcaaacacg tcattcgatt gatcagtgat
     3421 acctgaaatg ctctcaatga acg
//
