LOCUS       sequence-377725-       19180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for dCasEV2.1 activated expression for HarFAR, ATF1-2 and Atr
            delta11..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19180)
  AUTHORS   Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio
            S, Orzaez D, Patron N
  TITLE     Tunable control of insect pheromone biosynthesis in Nicotiana 
            benthamiana.
  JOURNAL   Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048.
  PUBMED    37032497
REFERENCE   2  (bases 1 to 19180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048."
COMMENT     Sequence Label: GB4367
FEATURES             Location/Qualifiers
     source          1..19180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1045..1069
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(1115..1367)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1407..2201)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1518..1537)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2128..2147
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2235..2414)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    13586..13610
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             14911..15540
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             15969..17042
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      17108..17302
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(17538..17560)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    17646..17786
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     17701..17720
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(17801..17818)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(17972..18560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18052..18071)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(18648..19180,1..259))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga
       61 tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat
      121 cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg
      181 tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg
      241 cgatcaccgc ttccctcata atgtttaact ttgttttagg gcgactgccc tgctgcgtaa
      301 catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg
      361 atgcccgagg catagactgt accccaaaaa aacagtcata acaagccatg aaaaccgcca
      421 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
      481 gctacttgca ttacagctta cgaaccgaac aggcttatgt ccactgggtt cgtgccttca
      541 tccgtttcca cggtgtgcgt cacccggcaa ccttgggtag cagcgaagtc gaggcatttc
      601 tgtcctggct ggaacagaac ttattatttc cttcctcttt tctacagtat ttaaagatac
      661 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
      721 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
      781 cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca
      841 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
      901 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
      961 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
     1021 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca taacggatcc
     1081 ggtctcagga gagcgatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg
     1141 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
     1201 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
     1261 attattacat gcttaacgta attcaacaga aattatatga taatcatggc aagaccggca
     1321 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag
     1381 ctagagtccg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggct
     1441 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     1501 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     1561 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     1621 ggcaagcagg cgtcgccgtg ggtcacgacg agatcctcgc cgtcgggcat ccgcgccttg
     1681 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     1741 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcggtgttt cgcttggtgg
     1801 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     1861 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     1921 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     1981 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt ggagttcatt cagggcaccg
     2041 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     2101 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     2161 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcctcgatc gagttgagag
     2221 tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt ggagcatttt
     2281 tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg cgcaataatg
     2341 gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctca gtggctcctt
     2401 caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg
     2461 gtcataccgt gactccctta attctcatgt atctccgtca ggagctaact gagatactac
     2521 catagatgac tacccatccc tctaggcctt agataaccgg atacagtgac tttgataggt
     2581 ttgtggggta cagctatgac ttgcatagct gcgtatggag gaaggaactt ttgcgtgtta
     2641 gtatgttgac ccgtgtatta cgcatgcggg tagatcatgt gggtagagac atccaggtca
     2701 agttctcgac cttctcgtgg gaggtgatcc agttcactat aggaccattc cattcgagca
     2761 tggcactaag tacgccgtcc ccattctggt aatcatcatc cctatcaggg tttggagtga
     2821 gtggtgacgg ttattcccca ggaatggact tcttattcac agtcggtcac attgggctac
     2881 tccttgggtc ttccgcttgg cccggtctgt tgggccgcca ttgcgtgagt ttcggccctg
     2941 tgctgcgctg tgtcggcgat tctcattggg gcctcacatc tggaaacccc gacctatttt
     3001 gacggcacca ttggcggaag ttgctgggct tgtgcaccgt gaagtcctcc gcgggcctcc
     3061 cccctacaaa agacgataag ctccggcaag cagcattgat caacgcaagg atcggcgata
     3121 ttaacagaga tacggctgat tactcttgtt ggtgtggtat cgctaaactg cgccgcggag
     3181 ccttatggca aagtcgtccg cgaaccattc cggtagcgct taaggtccat agcacattca
     3241 tcgcatcccg gcgtgcgttc aatttgacga ccccttggcg caaaggtgct ggccacgtgc
     3301 taaattaaag cggctgcact gctgtaaggt ccgttacgga gagggcggcc tggggagagc
     3361 actagcccat cgacctgtac aggaacactc tatattgttc tcggacggac agattactag
     3421 agtgccgctt tcagcccccc tgtcgtcgcc gacgtctgta atatggcgtt gttgtgattc
     3481 cactctattg aggcattaac tgatgcgtaa ggagatctgg aatgaactgg cctatgtcac
     3541 tgaaactgtg caaacaccca atgtcgttag tgtaggttct gaccgatacc tgcttcgttg
     3601 agaactcaca attttacaac tggggacata atccctacgc ccatcatcta ctcgcgtccc
     3661 tgtggctcca gttcatgtaa tgggagagta tcctccacaa gatctagtgc tatggtggta
     3721 tagtaagctc gtactgtggt agatgactcc ctcttctctc accaaccagt caacgccccg
     3781 taccgatgct gaacaagtcg atgcaggccc ttcttctctc accaaccagt ccaatcccga
     3841 ggcctgaccc gacatatccc atcttctctc accaaccagt cgtagctaac tatgttcccg
     3901 tcctaatacc tctacgtgca agcagtataa aaacaaaata cataaacata actaaaatct
     3961 catatcgttg taattgtcat atactagtgg taattaagtc cagttacact taaggttaaa
     4021 tttgctgact cttttttacc tttcatttct gaaaatggtt gtgttgacta gcaaggagac
     4081 aaagcctagc gtcgctgaat tttacgctgg aaaaagtgtt tttattactg ggggtactgg
     4141 gtttctcggt aaggttttca ttgagaaact cttgtattct tgtccagata tagaaaacat
     4201 ttacatgttg atcagggaaa aaaaaggttt atcagttggt gagaggatca agcagtttct
     4261 tgacgaccct ctgtttacca ggttgaagga taagaggcct gctgatcttg agaaaatcgt
     4321 ccttattcct ggcgacatta ctgctcccga cttgggtatt aacagtgaga acgaaaaaat
     4381 gcttatagaa aaggtgagtg ttatcataca ctcagcagct accgtcaagt tcaatgagcc
     4441 attgcctacc gcttggaaga tcaatgtcga gggtacacgg atgatgttag cacttagcag
     4501 acggatgaag agaatagagg ttttcatcca tatctctacc gcttacacaa atacaaacag
     4561 ggaggtggtt gatgaaattt tgtaccctgc acctgctgac attgaccaag ttcaccagta
     4621 cgtgaaagat ggtatctctg aagaagatac tgagaagatc ctcaatggaa ggcccaatac
     4681 atatactttc accaaggctc tgaccgagca tttagttgct gagaaccaag cttatgtgcc
     4741 aaccattatc gttaggccga gtgttgttgc tgctataaag gacgagccac ttaaaggatg
     4801 gctgggcaat tggtttggcg ctaccggatt gacagttttt accgcaaagg gactaaacag
     4861 ggtcatttat ggtcattcct catacatagt tgatcttatt cctgtagatt acgttgctaa
     4921 tttagttatc gccgcaggag ccaagtcttc aaaatctaca gaattgaaag tgtataattg
     4981 ctgctcttca agctgcaacc cagtgaccat tgggacgtta atgagtatgt tcgctgacga
     5041 cgctataaag cagaaaagtt atgcaatgcc gttaccgggt tggtacatat ttaccaagta
     5101 taagtggctt gtgctactgt tgactttctt atttcaggtc attcctgctt atgttacaga
     5161 tctctctagg catctgattg gtaaatcccc acgttatatc aagctccaat ccctggttaa
     5221 tcagactaga tcatctatag atttctttac caaccacagc tgggttatga aggccgaccg
     5281 ggtgagggag ttgtacgcat cactgtctcc cgccgataaa tacctattcc cgtgtgatcc
     5341 taccgatata aattggactc attatataca ggattattgt tggggtgtga ggcacttctt
     5401 ggaaaagaaa tatcggtgag cttctttgtg gttattattt agcttctgta cactaaattt
     5461 atgatgcaag aagcgttgta cacaacatat agaagaagag tgcgaggtga agcaagtagg
     5521 agaaatgtta ggaaagctcc tatacaaaag gatggcatgt tgaagattag catcttttta
     5581 atcccaagtt taaatataaa gcatatttta tgtaccactt tctttatctg gggtttgtaa
     5641 tccctttata tctttatgca atctttacgt tagttaatat ctatctatcg atattctagt
     5701 atcttatact atagatccaa ctgaaccaag aaattatgaa ccgtgtcttc cagaaattct
     5761 aataatgatg ggagcaatat aaatataagg atgtctttga caataaaagg gcggtggaag
     5821 agttatagtg aaagcttctg gccatcaaat gggctctttg tcacatgatt gaactacttc
     5881 accacctacc gttggtatcc atagtaaatt gagattaaaa aaagttaaaa caaactattc
     5941 attccatgtc attttacttt tcaacttgct atcttctaat tataagtggg ttgtggagag
     6001 agcactgttg ggtcagcaaa gcaaagtaga ttatactcta taatattatg atgattagtc
     6061 aactgctgct cacttacaac gatggaaatg gggatgaggg taactttacg ctgtcaagcg
     6121 gggtttagcc attaacatcg tcgtcaaagt tcgatttctg gagtgtgaaa aattagggtt
     6181 tcgtcgatag gagatgaaaa gtcgaaattt cggtgcgaga aatcaaaatt gatggaagat
     6241 cggcggcgaa tttgagagga tagtgagaag aagaagatta agagatgatt tgaattggga
     6301 gggaaaatcg aatcgccagt gtttgtttta gggtttttaa tctccgggaa ttgaattttg
     6361 ggcgtcgtaa aactgtaaag tgtgtttatg tagtaatctt tatctgagat gatacagaag
     6421 agattctatc ccagatacgg taaatttgtt ttcccctagg tcggtggtca acattgcatt
     6481 tttaagtcaa cttttaatat attttagttt aatttctata atttaacctt tttggcatta
     6541 tctttaggaa atttttttga cgtattttat tttacttatt aaaagtgaaa aatcttaaac
     6601 atattaatta atatcttgca taaccaattg acattgtaat catcacaatt aaatttgatt
     6661 aaaaatccac tttctgcaaa caaacaaagt tttttttaat aaagtctata acatatcgag
     6721 tataatagta tcaatataga ttttgatgat aacaacaata gccactcctg acggagtgtt
     6781 tcatgtccaa gcgaaaagcc gctctacgga atggatctac gttactgcct gcataaggag
     6841 atcggtgtag ccaaggacga aagcgaccct aggttctaac cgtcgacttt ggcggaaagg
     6901 tttcactcag gaagcagaca ctgattgaca cggtttagca gaacgtttga ggactaggtc
     6961 aaattgagtg gtttaatatc ggcatgtctg gctttaaaat tcagtatagt gcgctgatcg
     7021 gagatgaatt aaaaacacga gttcccaaaa ccaggcgggc tcgccacgtc ggctaatcct
     7081 ggtacattat gtgaacaatg ttctgaagaa aatttgtgaa agaaggacgg gtcatcgcct
     7141 actattagca acaacggtcg gccacacctt ccattgtcgt ggccacgctc ggattacacg
     7201 gcagaggtgc ttgtgttccg acaggctagc atattatcct aaggcgttac cccaatcgtt
     7261 taccgtcgga tttgctatag cccctgaacg ctacatgtac gaaaccatgt tatgtatgca
     7321 ctaggtcaac aataggacat agccttgtag ttaacacgta gcccggtcgt ataagtacag
     7381 tagacccttc gccggcatcc tatttattaa gttatttcta cagcaaaacg atcatatgca
     7441 gatccgcagt gggcggtaga cacacgtcca cccggctgct ctgtaacagg gactaaagag
     7501 gtgatgatta tcgtgtgtgc cccgttatgg tcgtgttcga tcagagcgcc cttgcgagca
     7561 gtcgtatgct ttctcgaatt ccgagcggtt aagcgtgaca gtcccagcga acccacaaaa
     7621 cgtgatcgca gtccatgcga tcatacgcaa gaaggaaggt ccccatacac cgacgcacca
     7681 gtttacacgc cgtatgcata aacgagctgc acaaacgaga gtgtttgaac tggacctcta
     7741 gttcctctac aaagaacacc ttgacctgtt gcgaagttgc cttgcctaga tgcaatgtcg
     7801 gacgtatcac ttttgcctca acgactgctg ctttcgctgt aacccaagac agacaacagt
     7861 aaccgccttt tgaaggcaag tcctccgcct gtgactaact gtgccaaatc gtcttccaaa
     7921 ccccttatcc aatttaactc accaaattat tgcgatacag accctaattt cacatcatat
     7981 gacactaatt gcctctgcca aaattctgtc ctcaatgact ccctcttctc tcaccaacca
     8041 gtcagttggg ttcacccggg tcggacctga gtcgaccaag ccttcttctc tcaccaacca
     8101 gtcgcgaccg cagaacttac gctcgagggt ccgtcttctc tcaccaacca gtctccccgg
     8161 ttatctctcc cgtcctaata cctctacgtg caagcagtat aaaaacaaaa tacataaaca
     8221 taactaaaat ctcatatcgt tgtaattgtc atatactagt ggtaattaag tccagttaca
     8281 cttaaggtta aatttgctga ctctttttta cctttcattt ctgaaaatga atgaaatcga
     8341 tgaaaaaaat caagctcccg tacaacagga gtgtctcaag gaaatgatac agaacggcca
     8401 cgcaaggagg atgggcagtg tagaggacct ctacgtagcc ttaaacagac aaaacctgta
     8461 tagaaacttt tgtacgtatg gggaactttc cgactactgc acccgagatc aactgacatt
     8521 agccctgagg gagatttgtc tcaaaaatcc aacccttctg catatagtct tgccaacccg
     8581 ttggccaaac catgagaact attatcgttc ttccgaatat tattcaagac cccatcctgt
     8641 ccatgattat ataagcgtac tccaagagtt gaagctttct ggggtagtgc ttaatgaaca
     8701 accggaatac tctgcagtga tgaagcaaat ccttgaagaa tttaagaatt caaaaggtag
     8761 ctatacggca aaaatcttca agttgacaac tactttgacg ataccctact tcgggcctac
     8821 cggtccttca tggagattga tctgtctccc cgaggaacat acagagaagt ggaggaagtt
     8881 catttttgtt agcaaccact gtatgtcaga tggcaggtcc agtatacatt ttttccatga
     8941 tcttagagat gagcttaaca atataaagac ccctccaaag aagttagact atatctttaa
     9001 gtatgaagag gattaccagt tattaaggaa actccctgag cctatagaga aagttataga
     9061 ctttaggcca ccgtatttat tcatcccgaa gagccttctt agcggattca tctataacca
     9121 cttacgattt agttccaaag gagtgtgtat gcgaatggat gacgttgaga aaacggatga
     9181 cgtcgtaaca gaaataataa atattagccc tacagaattt caagctatca aggccaatat
     9241 taagagtaac atccaaggga aatgtacgat cacgcccttt ttgcatgtgt gttggtttgt
     9301 atcccttcac aagtggggaa aatttttcaa gccccttaac ttcgaatggc tgactgacat
     9361 ttttatacca gctgattgca ggtcccaact cccagacgat gatgaaatgc gacagatgta
     9421 tagatatggc gctaatgtag ggtttataga cttcactccc tggataagcg agtttgatat
     9481 gaacgacaac aaagaaaact tctggccctt aattgaacac taccacgagg ttatttctga
     9541 agccttgagg aataagaagc atcttcatgg ccttggcttt aacattcagg gattcgttca
     9601 aaagtatgtc aatatagata aggttatgtg cgaccgtgct attggaaaaa gacgtggcgg
     9661 tacgctcttg agcaatgtgg gtttgtttaa tcaattggaa gagccggacg ccaaatattc
     9721 tatatgcgac ttagcctttg gtcaatttca gggaagctgg caccaagcct tcagtcttgg
     9781 agtatgtagc actaacgtca aaggaatgaa catagtggtt gcatcaacta aaaacgtggt
     9841 aggatctcag gagagcttag aggaattatg ttccatttac aaagctctgc tgttagggcc
     9901 ttaggcttac gaaaatatag ttgaaacaga gcagagatca tggtgttagc cttctatggc
     9961 tgaagaaaaa tagttgaaaa aatgtgtgtg tctagtgttt tagtgtgtct gtctgttgaa
    10021 aacagggaaa aaaatgtgtt cttttttccc tgtaataaga aatatagtaa tggagtcttt
    10081 atgtgtaagt aaccagtaaa atggtttgtg taaacatgaa tatctctgca tctgttaatt
    10141 ttaacatata agtttggata tgtgttgtgg tttcaactaa ttcaagtact tccttgataa
    10201 tgttttcctg tttctaggct gcaaaactat agtaaatctt ttaggttttg gtaaaaccta
    10261 attccattgg gtgtggttgg agagcatata gtttttgcga cgctctccgc tgtcaggagc
    10321 cctgattttt tcactactaa tagtactcac ggcgcaatac cagcacagcc tagtctcgcc
    10381 agaatgctgg tcagcatacg aaagagctta aggcaggcca attccactgt cagggtcact
    10441 tgggtgttta gcactaccga caggtacgct agtatgcgtt cttcctacca gaggtctgtg
    10501 gccgcgtggt caaaagtgcg gctttcgtat ttgctgctcg tgtttactct cacaaacttg
    10561 acctgcacgc caaagagatg cttcttgtgg aactcgacaa cgcaacaacg cgacggatct
    10621 acgtcacagc gtgcatagtg aaaacggagt tgctgacgac gaaagcgaca ttgggatctg
    10681 tcagttgtca ttcgcgaaaa acatccgtcc ccgaggcgga cactgattga cgcggttttg
    10741 tagaaggtta ggggaatagg ttagattgag tggcttaaga atgtaaaatc tgggattata
    10801 gtgtagtaat ctctgattaa cggtgacggt tttaagacag gtgttcgcaa aatcaagcgg
    10861 ggtcatttca acagatattg ctgatggttt aggcgtacaa tgccctgaag aataattaag
    10921 aaaaaagcac ccctcgtcgc ctagaattac ctaccgcggt ccaccatacc ttcgattatc
    10981 gcgcccactc tcccattagt cggcagaggt ggttgtgttg cgatagccca gtatgatatt
    11041 ctaaggcgtt acgctgatga atattctaca gagttgccat aggcgttgaa cgctacacgg
    11101 acgatacgaa tttacgtata gagcgggtca tcgaaaggtt atactctcgt agttaacatc
    11161 tagcccggcc ctatcagtac agcagtgcct tgaatgacat actcatcatt aaattttctc
    11221 tacagccaaa cgaccaagtg catttccagg gagcgcgatg gagattcatt ctctcgccag
    11281 cactgtaata ggcactaaaa gagtgatgat aatcatgagt gccgcgctaa ggtggtgtcg
    11341 gaacaaagcg gtcttacggt cagtcgtatt ccttctcgag ttccgtccag ttgagcgtgt
    11401 cactcccagt gtacctgcaa gccgagatgg ctgtgcttgg agtcaatcgc atgtagcatg
    11461 gtcaccagac accggggcac cagttttcac gcctaaagca taaacgacga gcagtcatga
    11521 aagtcttagt actggacgtg ccgtttcact gcgaatatga ctccctcttc tctcaccaac
    11581 cagtcacgac cgcttcacgc taaggtgctg gccacgtgct aaccttcttc tctcaccaac
    11641 cagtctggcg ttgatgtgga tcgactctat agccatcttc tctcaccaac cagtccgagg
    11701 ccgttcgttt cccgtcctaa tacctctacg tgcaagcagt ataaaaacaa aatacataaa
    11761 cataactaaa atctcatatc gttgtaattg tcatatacta gtggtaatta agtccagtta
    11821 cacttaaggt taaatttgct gactcttttt tacctttcat ttctgaaaat ggttcctaat
    11881 aagggttctt ctgatgtctt gtccgaacat tctgagccac agttcactaa gctgatcgcc
    11941 cctcaagccg gaccaaggaa gtataagatc gtgtaccgaa acttactgac tttcggatat
    12001 tggcaccttt ctgctgttta tggcttgtac ctttgtttca cttgtgctaa atgggcaacg
    12061 attttgtttg ccttttttct ttacgtgata gctgaaattg ggattactgg tggagctcac
    12121 agactttggg cacatagaac ttacaaggct aaactgcccc tagaaattct tcttctcatt
    12181 atgaactcca tagcttttca agatactgca tttacttggg ccagggacca tagacttcac
    12241 cacaaatact cagatactga tgcagatcct cacaacgcaa cccggggttt tttttattcc
    12301 cacgttgggt ggttgttggt caaaaagcat ccagaggtta aggctagagg aaagtacttg
    12361 tctctcgatg acttaaagaa taacccgcta ttgaagttcc aaaaaaaata cgccatactc
    12421 gtaattggaa ccttatgttt tttgatgcct acgttcgtcc cggtatactt ttggggtgaa
    12481 ggcatctcta ctgcatggaa catcaatctt ttacgatatg tcatgaactt aaatatgact
    12541 ttcctagtca actctgctgc ccatatattt gggaacaagc cgtatgataa atcaatagct
    12601 tctgtgcaaa atatatcagt ttccctggct acatttgggg agggtttcca taattatcat
    12661 cacacatatc cctgggatta tcgtgcagca gaacttggaa acaatagatt gaatatgaca
    12721 accgccttta ttgatttctt cgcctggata ggttgggcat atgatctaaa gtctgtgcca
    12781 caagaggcta ttgcaaaacg gtgcgcaaaa actggagatg gtactgatat gtggggtagg
    12841 aaacgttgag cttaaaccca accatacata ataaaaaggc gagctacctc accaacatgt
    12901 tcttggttct tgatggctgc ttgaggtttt tcaacacaac atgagtaaaa tggtcaataa
    12961 atatggatat tctcatcttg cttgattcgg gcaggtagga tatcagagaa ttctaggtat
    13021 attttgtagg gaaaaaaaac tagaatctct tcaggatctg gatcctaata caaggacata
    13081 ttaatattcc attttataaa ctttgcacaa gcaagatcta attttatgga agaactgaag
    13141 gctctcgcgt atcatttgcc aaaataatta ccatcttttg taatcatctt ggaaactaga
    13201 gataatattg ataatatcca tagtaataat aataataaga agaacaaata tcatcataac
    13261 acaataatat agccttttca tgtaaaattt catttcaatg aatagccatg ttagcccatt
    13321 ttctttcagg aaagtttatg cttgcttaca tgaagaaaca aaaaaaatta tgagaaacag
    13381 aatttactca ctactgtgca aattatatgt tcaactgagc aaacaagtct tcaactacct
    13441 ctcctcagag ttgaatctgt tccatgcttc ctatagacac aaaagaacga taaccactaa
    13501 gataattcaa agaagataat aaaagagaat ggaccatggg aagaagaggg ggaggtgaag
    13561 ctggcgctgt catgagaccg gatcctgaca ggatatattg gcgggtaaac ctaagagaaa
    13621 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
    13681 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac
    13741 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtca tctgaaaacg
    13801 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt
    13861 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt
    13921 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg
    13981 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt
    14041 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc
    14101 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg
    14161 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag
    14221 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca
    14281 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca
    14341 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg
    14401 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg
    14461 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg
    14521 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg
    14581 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc
    14641 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc
    14701 gcccgcgcac ctctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa
    14761 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag
    14821 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg
    14881 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa
    14941 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg
    15001 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg
    15061 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg
    15121 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg
    15181 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac
    15241 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat
    15301 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt
    15361 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag
    15421 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag
    15481 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga
    15541 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga
    15601 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc
    15661 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc
    15721 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga
    15781 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa
    15841 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca
    15901 ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag
    15961 gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg
    16021 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag
    16081 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc
    16141 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag
    16201 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg
    16261 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
    16321 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
    16381 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
    16441 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
    16501 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
    16561 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
    16621 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
    16681 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga
    16741 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
    16801 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
    16861 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
    16921 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
    16981 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
    17041 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac
    17101 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc
    17161 cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata
    17221 taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta
    17281 aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag
    17341 cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg
    17401 ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag
    17461 ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc
    17521 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg tgacggtcac agcttgtctg
    17581 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    17641 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
    17701 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    17761 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
    17821 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    17881 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    17941 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    18001 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    18061 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    18121 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    18181 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    18241 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    18301 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    18361 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
    18421 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    18481 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    18541 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    18601 ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtgatta tttgccgact
    18661 accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag
    18721 gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga
    18781 tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg
    18841 ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcacca
    18901 gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc
    18961 tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt
    19021 tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata
    19081 cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa
    19141 cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt
//
